The preoptico-infundibular LH-RH tract of the rat

Merchenthaler, Istvån; Kovács, Gyula; Lovász, György; Sétáló, G.

doi:10.1016/0006-8993(80)90344-3

Cited by 116 publications

(52 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Fluorescent GnRH neurons and their processes are seen in expected areas, and their distribution in the mediobasal hypothalamus (i.e. the ARC and ME) matches that of GnRH immunoreactive processes described previously [2]. One of the advantages of the GnRH-GFP mice is that they facilitate investigation of the precise relationships between GnRH axons and identified somata and processes in the mediobasal hypothalamus.…”

Section: Introductionmentioning

confidence: 75%

“…The axons of GnRH neurons terminate on fenestrated capillaries where GnRH is released into the portal blood. This system of GnRH neurons has been termed the septo-preoptico-tuberoinfundibular pathway [1, 2, 3]. Because the GnRH pathway courses across the mediobasal hypothalamus and throughout the arcuate nucleus (ARC), it is possible that GnRH modulates the activity of ARC neurons by ‘en passant’ contacts.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A Confocal Microscopic Study of Gonadotropin-Releasing Hormone (GnRH) Neuron Inputs to Dopaminergic Neurons Containing Estrogen Receptor α in the Arcuate Nucleus of GnRH-Green Fluorescent Protein Transgenic Mice

et al. 2003

View full text Add to dashboard Cite

The purpose of the present study was to determine whether the septo-preoptico-tuberoinfundibular gonadotropin-releasing hormone (GnRH) pathway comes in close juxtaposition with tyrosine hydroxylase immunoreactive (TH-IR) neurons in the arcuate nucleus of female mice. Immunohistochemical staining with a TH monoclonal antibody coupled with confocal microscopy was employed on vibratome-cut brain sections of female GnRH-green fluorescent protein (GFP) transgenic mice to evaluate possible appositions between GnRH and tuberoinfundibular dopaminergic (TIDA) neurons. TH-IR neurons of the arcuate nucleus received GnRH neuronal appositions in adult female mice at proestrus and estrus stages. In contrast, no GnRH appositions were observed in adult females at diestrus. Subsequently, double immunohistochemical staining for TH and estrogen receptor-α (ERα) was performed to examine the role of estradiol on this relationship. We found that most TH-IR neurons contacted by GnRH fibers were immunoreactive for ERα. Our observations suggest that GnRH neurons communicate directly with TIDA neurons in the adult female. Furthermore, ERα activation in TIDA neurons may be involved in the formation of connections between GnRH neurons and TIDA neurons.

show abstract

Section: Introductionmentioning

confidence: 75%

Section: Introductionmentioning

confidence: 99%

A Confocal Microscopic Study of Gonadotropin-Releasing Hormone (GnRH) Neuron Inputs to Dopaminergic Neurons Containing Estrogen Receptor α in the Arcuate Nucleus of GnRH-Green Fluorescent Protein Transgenic Mice

et al. 2003

View full text Add to dashboard Cite

show abstract

“…Immunocytochemical studies have revealed, however, that the majority of the perikarya of LHRH neurons are located in the preoptic and suprachiasmatic regions (Setalo et al, 1976;King et al, 1982;Hoffman and Gibbs, 1982 This coincidence indicated that the effect of anterior deafferentations or lesions of the preoptic area in inducing persistent estrus might be due to impairment of the LHRH neurosecretory neurons. Indeed, anterior deafferentations of the MBH (Merchenthaler et al, 1980a) or lesions of the ventral part of the preoptic area (Ibata et al, 1979;Popolow et al, 1981;Krey and Silverman, 1981) reduced the concentration of immunoreactive LHRH fibers in the median eminence, although it should be noted that a substantial proportion of the fibers were spared.…”

Section: Discussionmentioning

confidence: 99%

The LHRH neuronal system in female rats: Relation to the medial preoptic nucleus.

Terasawa¹,

Davis²

1983

Endocrinol Japon

View full text Add to dashboard Cite

Previously we have found that small lesions confined to the medial preoptic nucleus (MPN) or the suprachiasmatic nucleus (SCN) blocked the cyclic release of gonadotropins in the female rat, inducing a persistent estrous state. Since the MPN is located just caudal to the organum vasculosum of the lamina terminalis (OVLT) where LHRH cell bodies are most concentrated, we applied an immunocytochemical technique to examine the possibility that the lesions had simply disrupted LHRH neurons or fibers.Using a new anti-LHRH provided by Dr. V. D. Ramirez, we found that the distribution pattern of immunoreactive LHRH cell bodies and fibers was similar to that previously reported, although the staining was more intense and extensive with low background.There was no concentration of LHRH cell bodies and fibers in the MPN or SCN and, in fact, these nuclei generally showed a lower density of stained elements than did sorrounding tissue. In persistent estrous animals with lesions confined to the MPN there was no detectable reduction of staind fibers in the median eminence.These results, along with the results of other workers, suggest that persistent estrus following lesions of the MPN or SCN is not due to reduction of LHRH neurons or fibers. Rather, they support the hypothesis that these nuclei are critical for triggering the ovulatory release of LHRH.

show abstract

“…However, most studies previously performed failed to reveal immunoreactive LHRH perikarya in rats kept under physiological conditions (KING et al, 1974;BAKER and DERMODY, 1976;SETALO et al, 1976;DAIKOKU et al, 1978;MERCHENTHALER et al, 1980a). One possible explanation for the failure in immunohistochemical presentation of the perikarya in intact adult rats is that the transport of LHRH from the perikarya may be so rapid that the LHRH concentration therein is too low for immunohistochemical detection (BAKER et al, 1975).…”

mentioning

confidence: 99%

Immunohistochemical demonstration of LHRH-neurons in young rat hypothalamus: Light and electron microscopy.

Daikoku

Hisano

Maki

1982

Arch. Histol. Jap., Arch. Histol. Jpn

View full text Add to dashboard Cite

The preoptico-infundibular LH-RH tract of the rat

Cited by 116 publications

References 17 publications

A Confocal Microscopic Study of Gonadotropin-Releasing Hormone (GnRH) Neuron Inputs to Dopaminergic Neurons Containing Estrogen Receptor α in the Arcuate Nucleus of GnRH-Green Fluorescent Protein Transgenic Mice

A Confocal Microscopic Study of Gonadotropin-Releasing Hormone (GnRH) Neuron Inputs to Dopaminergic Neurons Containing Estrogen Receptor α in the Arcuate Nucleus of GnRH-Green Fluorescent Protein Transgenic Mice

The LHRH neuronal system in female rats: Relation to the medial preoptic nucleus.

Immunohistochemical demonstration of LHRH-neurons in young rat hypothalamus: Light and electron microscopy.

Contact Info

Product

Resources

About