The predominant cultivable microbiota of active and inactive lesions of destructive periodontal diseases

Dzink, J. L.; Socransky, S. S.; Haffajee, A. D.

doi:10.1111/j.1600-051x.1988.tb01590.x

Cited by 664 publications

(455 citation statements)

References 46 publications

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“…Since these organisms have an important role in oral infection, their isolation and identification are required (12,18). Periodontal bacteria such as E. corrodens, A. actinomycetemcomitans, P. gingivalis, P. intermedia and F. nucleatum are recognized as important opportunistic pathogens in the development of periodontal and non-oral diseases of humans and dogs (7,16,19).…”

Section: Discussionmentioning

confidence: 99%

Detection of putative periodontal pathogens in subgingival specimens of dogs

Nishiyama¹,

Senhorinho²,

Gioso³

et al. 2007

Braz. J. Microbiol.

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In this study, the presence of putative periodontal organisms, Porphyromonas gingivalis, Prevotella intermedia, Tannerella forsythensis, Fusobacterium nucleatum, Dialister pneumosintes, Actinobacillus actinomycetemcomitans, Campylobacter rectus, Eikenella corrodens and Treponema denticola were examined from subgingival samples of 40 dogs of different breeds with (25) and without (15) periodontitis, by using the PCR method. The PCR products of each species showed specific amplicons. Of the 25 dogs with periodontitis, P. gingivalis was detected in 16 (64%) samples, C. rectus in 9 (36%), A. actinomycetemcomitans in 6 (24%), P. intermedia in 5 (20%), T. forsythensis in 5 (20%), F. nucleatum in 4 (16%) and E. corrodens in 3 (12%). T. denticola and D. pneumosintes were not detected in clinical samples from dogs with periodontitis. Moreover, P. gingivalis was detected only in one (6.66%) crossbred dog without periodontitis. Our results show that these microorganisms are present in periodontal microbiota of dogs with periodontitits, and it is important to evaluate the role of these putative periodontal microorganisms play in the periodontitis in household pets particularly, dogs in ecologic and therapeutic terms, since these animals might acquire these periodontopahogens from their respective owners.

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Section: Discussionmentioning

confidence: 99%

Detection of putative periodontal pathogens in subgingival specimens of dogs

Nishiyama¹,

Senhorinho²,

Gioso³

et al. 2007

Braz. J. Microbiol.

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“…A shift in the microbial community leading to outgrowth of this anaerobe is directly linked to periodontitis, a chronic inflammatory disease that leads to destruction of the tissues supporting the gums and ultimately, exfoliation of the teeth (Choil et al, 1990;Dzink et al, 1988;Grossi et al, 1994;Lamont & Jenkinson, 2000;Moore et al, 1991). This commensal can colonize, invade and multiply within gingival epithelial cells, as well as penetrate into deeper epithelial cell layers, potentially releasing the whole organism and/or virulence factors into the bloodstream (reviewed by Yilmaz, 2008).…”

Section: Introductionmentioning

confidence: 99%

The nucleoid-associated protein HUβ affects global gene expression in Porphyromonas gingivalis

et al. 2013

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HU is a non-sequence-specific DNA-binding protein and one of the most abundant nucleoidassociated proteins in the bacterial cell. Like Escherichia coli, the genome of Porphyromonas gingivalis is predicted to encode both the HUa (PG1258) and the HUb (PG0121) subunit. We have previously reported that PG0121 encodes a non-specific DNA-binding protein and that PG0121 is co-transcribed with the K-antigen capsule synthesis operon. We also reported that deletion of PG0121 resulted in downregulation of capsule operon expression and produced a P. gingivalis strain that is phenotypically deficient in surface polysaccharide production. Here, we show through complementation experiments in an E. coli MG1655 hupAB double mutant strain that PG0121 encodes a functional HU homologue. Microarray and quantitative RT-PCR analysis were used to further investigate global transcriptional regulation by HUb using comparative expression profiling of the PG0121 (HUb) mutant strain to the parent strain, W83. Our analysis determined that expression of genes encoding proteins involved in a variety of biological functions, including iron acquisition, cell division and translation, as well as a number of predicted nucleoid associated proteins were altered in the PG0121 mutant. Phenotypic and quantitative real-time-PCR (qRT-PCR) analyses determined that under iron-limiting growth conditions, cell division and viability were defective in the PG0121 mutant. Collectively, our studies show that PG0121 does indeed encode a functional HU homologue, and HUb has global regulatory functions in P. gingivalis; it affects not only production of capsular polysaccharides but also expression of genes involved in basic functions, such as cell wall synthesis, cell division and iron uptake. INTRODUCTIONPorphyromonas gingivalis is a Gram-negative obligate anaerobe belonging to the family Bacteroidaceae that persists as a natural member of the human oral microbiota. A shift in the microbial community leading to outgrowth of this anaerobe is directly linked to periodontitis, a chronic inflammatory disease that leads to destruction of the tissues supporting the gums and ultimately, exfoliation of the teeth (Choil et al., 1990;Dzink et al., 1988;Grossi et al., 1994;Lamont & Jenkinson, 2000;Moore et al., 1991). This commensal can colonize, invade and multiply within gingival epithelial cells, as well as penetrate into deeper epithelial cell layers, potentially releasing the whole organism and/or virulence factors into the bloodstream (reviewed by Yilmaz, 2008). In addition to its ability to cause disease in the oral cavity, there are data indicating a role in systemic disease, including its ability to invade vascular endothelial cells (Dorn et al., 2000(Dorn et al., , 2002Jandik et al., 2008) and to cause aggregation of platelets (Pham et al., 2002). For many pathogenic bacteria, surface polysaccharides play a key role in immune modulation et al., 1996). HU typically acts as an accessory protein in virtually all types of nucleoprotein-mediated processes (reviewed by...

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“…Fusobacterium species play a central role in biofilm formation, mediating coaggregation between early and late colonizers (5,6,7). Fusobacterium species including F. nucleatum, are detected in high frequency in lesions of gingivitis and chronic periodontitis (8,9). P. intermedia and Prevotella nigrescens are frequently isolated from the periodontal lesions of patients with chronic periodontitis (8,9,10), aggressive periodontitis (11,12), puberty-associated gingivitis (13,14), and acute necrotizing ulcerative gingivitis (15).…”

Section: Introductionmentioning

confidence: 99%

“…Fusobacterium species including F. nucleatum, are detected in high frequency in lesions of gingivitis and chronic periodontitis (8,9). P. intermedia and Prevotella nigrescens are frequently isolated from the periodontal lesions of patients with chronic periodontitis (8,9,10), aggressive periodontitis (11,12), puberty-associated gingivitis (13,14), and acute necrotizing ulcerative gingivitis (15). Recently, our research group reported that the average number of periodontopathic bacterial species, including P. intermedia and P. nigrescens, was significantly higher in plaque samples 5 harboring F. nucleatum and/or F. periodontium than in those without in Japanese children and mothers (16).…”

Section: Introductionmentioning

confidence: 99%

Synergy in biofilm formation between Fusobacterium nucleatum and Prevotella species

et al. 2012

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Japan Running title: F. nucleatum-Prevotella species CoaggregationThe aim of this study was to characterize the role of coaggregation between Fusobacterium nucleatum and Prevotella species in biofilm formation. Materials and Methods:ATCC and clinically isolated strains of F. nucleatum, P.intermedia and P. nigrescens were subjected. Coaggregation between these species was determined by visual assay. Biofilm formation was assessed by crystal violet staining. Effect of co-culture of F. nucleatum with Prevotella species was evaluated. Enhancement of biofilm formation via soluble factor was also examined by addition of culture supernatant and a two-compartment separated co-culture system. Production of autoinducer-2 (AI-2) by the tested organisms was evaluated using Vibrio harveyi BB170.Results: Cells of all F. nucleatum strains coaggregated with P. intermedia or P.nigrescens. Addition of EDTA or L-lysine inhibited coaggregation, the other sugars and amino acids tested did not. Coaggregation disappeared after heating of P. intermedia and P. nigrescens cells, but not after heating of F. nucleatum cells. Proteinase K treatment of P. nigrescens cells affected coaggregation with F. nucleatum, but no significant effect was observed with the treatment of cells of P. intermedia or F. nucleatum. The quantities of biofilm formation by strains of F. nucleatum, P. intermedia and P. nigrescens varied.Co-culture of F. nucleatum ATCC 25586 with P. intermedia or P. nigrescens strains increased biofilm formation than by the single culture (p<0.05).Production levels of AI-2 in strains of F. nucleatum, P. intermedia and P.3 nigrescens varied. Addition of culture supernatant of P. intermedia or P.nigrescens did not enhance biofilm formation by F. nucleatum, and no biofilm formation enhancement by F. nucleatum was observed by the two-compartment co-culture system. Conclusion:These findings indicate that physical contact by coaggregation of F.nucleatum strains with P. intermedia or P. nigrescens is the key factor in the formation of biofilm by these strains, rather than the AI-2 signaling molecule. 4 IntroductionDental plaque, a multispecies biofilm, is organized on the tooth surfaces and periodontal tissues of the human oral cavity (1). More than 500 taxa of bacteria have been identified in the human oral cavity (2), and an increase in specific anaerobic, Gram-negative bacteria in the biofilm is involved in the progression of periodontal disease (3). Bacterial gene expression in the biofilm is regulated by quorum sensing according to increase in cell density (1). After incorporation into the biofilm, microorganisms become resistant not only to host defense mechanisms, but also antimicrobial agents. Moreover, this change has been suggested to be involved in the persistence of infection by such microorganisms in the subgingival crevice (4).In dental plaque biofilm formation, early colonizers, including streptococci, Coaggregation assayVisual assay of coaggregation of F. nucleatum with P. intermedia or P. leaving a water-clear supernatant...

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The predominant cultivable microbiota of active and inactive lesions of destructive periodontal diseases

Cited by 664 publications

References 46 publications

Detection of putative periodontal pathogens in subgingival specimens of dogs

Detection of putative periodontal pathogens in subgingival specimens of dogs

The nucleoid-associated protein HUβ affects global gene expression in Porphyromonas gingivalis

Synergy in biofilm formation between Fusobacterium nucleatum and Prevotella species

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