The precore mutation is associated with severity of liver damage in Brazilian patients with chronic hepatitis B

Rezende, Rosamar Eulira Fontes; Fonseca, Benedito Antônio Lopes da; Ramalho, Leandra Náira Zambelli; Zucoloto, Sérgio; Pinho, João Renato Rebello; Bertolini, Dennis Armando; Martinelli, Ana de Lôurdes Candolo

doi:10.1016/j.jcv.2004.08.001

Cited by 34 publications

(39 citation statements)

References 43 publications

(48 reference statements)

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“…In contrast, all HBeAg positive patients had wild-type virus with normal liver enzymes, but only two patients, increase of liver enzymes was observed, whereas these two patients were categorized into chronic hepatitis B and cirrhosis group. A similar result was observed by the Kidd-Ljunggren et al [2004] who found that genotype D precore mutant infected patients had high levels of ALT in comparison to HBeAg positive patients and similar results were observed in other investigations [Chu et al, 2002;Funk et al, 2002;Rezende et al, 2005].…”

Section: Discussionsupporting

confidence: 89%

“…Recently, investigations have confirmed that there are relationships between these mutations in the BCP/preC region and the outcome of HBV infection. Among HBV genotypes, genotype D of HBV has shown more frequent precore mutations and consequently HBeAg negative status in infected patients and is associated with a greater severity of liver disease [Kidd-Ljunggren et al, 2004;Marcellin et al, 2005;Rezende et al, 2005]. In this study, all HBeAg negative patients have a precore or mixed (wildtype plus mutant) HBV strains with the exception of Afgh-5 isolates that showed wild-type with active cirrhosis.…”

Section: Discussionmentioning

confidence: 57%

“…The results showed the presence of HBV genotype D (95% bootstrap value), sub-genotype D1 (97% bootstrap value), and subtype ayw2 in all Afghan HBV infected patients. The HBV genotype D is distributed worldwide, and has been reported frequently in Mediterranean countries [Saudy et al, 2003;Sallam and Tong, 2004], the Middle East countries [Amini-Bavil-Olyaee et al, 2005a,b;Bozdayi et al, 2005] and is spreading to India [Thakur et al, 2002[Thakur et al, , 2005Banerjee et al, 2005], China [Xu et al, 2003], North America [Kato et al, 2004], South America as Venezuela, Argentina, and Brazil [Blitz et al, 1998;Mbayed et al, 1998;Rezende et al, 2005], and Europe including Spain [Echevarria et al, 2005], Scotland [Davidson et al, 2005], Czech [Krekulova et al, 2004], Poland [Bielawski et al, 2004], France [Grandjacques [Micalessi et al, 2005], and Russia [Abe et al, 2004]. Genotype D HBV has been classified into four divergent sub-genotypes, designated by D1 to D4 that showed geographical and subtype distribution [Norder et al, 2004].…”

Section: Discussionmentioning

confidence: 99%

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Hepatitis B virus genotyping, core promoter, and precore/core mutations among Afghan patients infected with hepatitis B: A preliminary report

et al. 2006

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In spite of hepatitis B virus (HBV) vaccination, HBV infection remains an important public health problem worldwide. Although the HBV genotype distribution has been determined in some parts of South Central Asia, no survey has been conducted to determine the HBV genotype in Afghanistan. Twelve Afghan patients infected with HBV living in Afghanistan were enrolled in this study. Partial HBsAg and basic core promoter, precore, and core (BCP/preC/C) regions were amplified and subjected for direct sequencing. In parallel, precore G1896A mutation was also determined by an amplification-created restriction site method. Results revealed HBV genotype D (95% bootstrap value), sub-genotype D1 (98% bootstrap value), and subtype ayw2 in all Afghan isolates. Afghan isolates clustered in a separate branch in the D1 sub-genotype called D1', while supported by 82% bootstrap value. The percentage of intra-genotypic distance among Afghan isolates was 1.05% and inter-genotypic distance with the other genotype D was 2.87% and with other genotypes was 7.50%-11.1%. The wild-type, mixed infection, and precore mutant were found in six, two, and four HBV isolates, respectively. The A1762T/G1764A BCP dual mutation was found in one isolate. Three isolates presented single mutation in the BCP dual mutation region, whereas two showed a novel G1764T mutation. In conclusion, this preliminary study revealed HBV genotype D, sub-genotype D1, and subtype ayw2 of HBV among hepatitis B infected patients from Afghanistan. Further investigation should be carried out.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 57%

Section: Discussionmentioning

confidence: 99%

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Hepatitis B virus genotyping, core promoter, and precore/core mutations among Afghan patients infected with hepatitis B: A preliminary report

et al. 2006

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“…In our study, we observed the presence of these genotypes. Although our data differ in genotype distribution, in relation to data from other regions in Brazil, these results are concordant with another study reported by Resende et al 39 from the same region, where genotype D is the most prevalent followed by genotypes A and F.…”

Section: Referencessupporting

confidence: 91%

Hepatitis B virus genotyping among chronic hepatitis B patients with resistance to treatment with lamivudine in the City of Ribeirão Preto, State of São Paulo

Haddad¹,

Martinelli

Uyemura

et al. 2010

Rev. Soc. Bras. Med. Trop.

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Introduction: Lamivudine is a nucleoside analogue that is used clinically for treating chronic hepatitis B infection. However, the main problem with prolonged use of lamivudine is the development of viral resistance to the treatment. Mutations in the YMDD motif of the hepatitis B virus DNA polymerase gene have been associated with resistance to drug therapy. So far, there have not been many studies in Brazil reporting on genotype-dependent development of resistance to lamivudine. Thus, the aim of the present study was to determine the possible correlation between a certain genotype and increased development of resistance to lamivudine among chronic hepatitis B patients. Methods: HBV DNA in samples from 50 patients under lamivudine treatment was amplified by means of conventional PCR. Samples were collected at Hospital das Clínicas, FMRP-USP. The products were then sequenced and phylogenetic analysis was performed. Results: Phylogenetic analysis revealed that 29 (58%) patients were infected with genotype D, 20 (40%) with genotype A and one (2%) with genotype F. Mutations in the YMDD motif occurred in 20% of the patients with genotype A and 27.6% of the patients with genotype D. Conclusions: Despite the small number of samples, our results indicated that mutations in the YMDD motif were 1.38 times more frequent in genotype D than in genotype A.

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“…The genetic heterogeneity of HBV, including genotypes and mutations, may play an important role in the clinical outcome of HBV infection [Rezende et al, 2005]. It was suggested that HBV genotypes might influence the clinical features of HBV infection [Kao et al, 2000] although the mechanisms of the genotype-related differences in the pathogenicity of HBV remain largely unknown.…”

Section: Introductionmentioning

confidence: 99%

Hepatitis B genotypes, precore and core promoter mutants circulating in Tunisia

et al. 2006

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Hepatitis B virus (HBV) is characterized by genetic heterogeneity, including genotypes and mutations. Eight genotypes (A-H) have been identified throughout the world with a characteristic geographical distribution. Previous studies also suggest that the viral genotypes may correlate with differences in clinical features of the infection. Two types of mutations were particularly described, precore and basal promoter mutations; they may play an important role in the clinical outcome of HBV infection. The aim of this study was to investigate the prevalence of HBV genotypes and HBV variants in Tunisia, and their eventual association with severity of liver disease. Using a molecular method, HBV genotypes, precore and basal core promoter mutations were determined in 56 asymptomatic carriers and in 82 patients with histologically verified chronic liver disease and hepatocellular carcinoma (HCC). Three genotypes (D, A, and E) were detected; the prevalence was 80%, 8%, and 9%, respectively. No significant difference was observed for genotype D with clinical status. HBV mutants were detected in 93% of cases, precore mutants were the most prevalent. Basal core promoter mutants were observed in 61% of cases, they were frequently characterized by a double mutation in 1762 and 1764. Co-infection by these two types of mutants was detected in 50% of cases. Genotype D was the most prevalent HBV genotype in Tunisia. High circulation of precore and basal core promoter mutants are common in chronic hepatitis B infection in Tunisia.

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The precore mutation is associated with severity of liver damage in Brazilian patients with chronic hepatitis B

Cited by 34 publications

References 43 publications

Hepatitis B virus genotyping, core promoter, and precore/core mutations among Afghan patients infected with hepatitis B: A preliminary report

Hepatitis B virus genotyping, core promoter, and precore/core mutations among Afghan patients infected with hepatitis B: A preliminary report

Hepatitis B virus genotyping among chronic hepatitis B patients with resistance to treatment with lamivudine in the City of Ribeirão Preto, State of São Paulo

Hepatitis B genotypes, precore and core promoter mutants circulating in Tunisia

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