The precerebellar linear nucleus in the mouse defined by connections, immunohistochemistry, and gene expression

Fu, Yu Hong; Tvrdík, Petr; Makki, Nadja; Palombi, Olivier; Machold, Robert; Paxinos, George; Watson, Charles

doi:10.1016/j.brainres.2009.02.068

Cited by 13 publications

(21 citation statements)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In a recent study, we have documented the projection of the linear nucleus to the cerebellum [26]. The linear nucleus is a very distinctive cell group in rodents, but is not consistently found in other mammals [26].…”

Section: Discussionmentioning

confidence: 97%

“…The linear nucleus is a very distinctive cell group in rodents, but is not consistently found in other mammals [26]. The linear nucleus appears to be a specialized extension of the lateral reticular nucleus.…”

Section: Discussionmentioning

confidence: 98%

“…3). The linear nucleus of the hindbrain, which is almost certainly a rostral extension of LRt [26], is prominently labeled on both sides after unilateral cerebellar injections.…”

Section: Retrograde Labeling In Major Precerebellar Nucleimentioning

confidence: 99%

See 2 more Smart Citations

Precerebellar Cell Groups in the Hindbrain of the Mouse Defined by Retrograde Tracing and Correlated with Cumulative Wnt1-Cre Genetic Labeling

Tvrdík

Makki

et al. 2011

Cerebellum

Self Cite

View full text Add to dashboard Cite

The precerebellar nuclei are hindbrain and spinal cord centers that send fibers to the cerebellum. The neurons of the major hindbrain precerebellar nuclei are derived from the rhombic lip. Wnt1, a developmentally important gene involved in intercellular signaling, is expressed in the developing rhombic lip. We sought to investigate the relationship between the cell clusters expressing Wnt1 and the precerebellar nuclei in the hindbrain. We therefore defined the hindbrain precerebellar nuclei by retrograde tracing, following cerebellar injections of HRP, and compared these results with the cell clusters expressing Wnt1 in newborn mice. We found that 39 distinct hindbrain nuclei project to the cerebellum. Of these nuclei, all but three (namely the oral pontine reticular nucleus, the caudal pontine reticular nucleus, and the subcoeruleus nucleus) contain neurons expressing Wnt1. This shows a high degree of overlap between the precerebellar nuclei and the nuclei that express Wnt1. However, it should be noted that neurons expressing Wnt1 are also found in the superior olivary complex, which is a basal plate derivative lacking cerebellar projections.

show abstract

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 98%

See 1 more Smart Citation

Precerebellar Cell Groups in the Hindbrain of the Mouse Defined by Retrograde Tracing and Correlated with Cumulative Wnt1-Cre Genetic Labeling

Tvrdík

Makki

et al. 2011

Cerebellum

Self Cite

View full text Add to dashboard Cite

show abstract

“…Genetic strategies in mice extended the classical chick studies and revealed that dA1/Atoh1 ϩ neurons along the cRL assemble the ventral/dorsal CN and PCN (Wang et al, 2005;Okada et al, 2007;Fu et al, 2009Fu et al, , 2011Rose et al, 2009b). Application of intersectional/subtractive fate-map approaches further identified the contribution of rhombomere-specific interneurons to these nuclei (Farago et al, 2006;Maricich et al, 2009).…”

Section: Axonal Patterns Targets and Connectivity Of Da1-interneuronsmentioning

confidence: 90%

Axonal Patterns and Targets of dA1 Interneurons in the Chick Hindbrain

Kohl

Hadas²,

Klar³

et al. 2012

J. Neurosci.

View full text Add to dashboard Cite

Hindbrain dorsal interneurons that comprise the rhombic lip relay sensory information and coordinate motor outputs. The progenitor dA1 subgroup of interneurons, which is formed along the dorsal-most region of the caudal rhombic lip, gives rise to the cochlear and precerebellar nuclei. These centers project sensory inputs toward upper-brain regions. The fundamental role of dA1 interneurons in the assembly and function of these brainstem nuclei is well characterized. However, the precise en route axonal patterns and synaptic targets of dA1 interneurons are not clear as of yet. Novel genetic tools were used to label dA1 neurons and trace their axonal trajectories and synaptic connections at various stages of chick embryos. Using dA1-specific enhancers, two contralateral ascending axonal projection patterns were identified; one derived from rhombomeres 6 -7 that elongated in the dorsal funiculus, while the other originated from rhombomeres 2-5 and extended in the lateral funiculus. Targets of dA1 axons were followed at later stages using PiggyBac-mediated DNA transposition. dA1 axons were found to project and form synapses in the auditory nuclei and cerebellum. Investigation of mechanisms that regulate the patterns of dA1 axons revealed a fundamental role of Lim-homeodomain (HD) proteins. Switch in the expression of the specific dA1 Lim-HD proteins Lhx2/9 into Lhx1, which is typically expressed in dB1 interneurons, modified dA1 axonal patterns to project along the routes of dB1 subgroup. Together, the results of this research provided new tools and knowledge to the assembly of trajectories and connectivity of hindbrain dA1 interneurons and of molecular mechanisms that control these patterns.

show abstract

“…The linear nucleus (Fig. 26A,B) was included in the lateral reticular nucleus on the basis of cytological, enzyme histochemical, and anterograde and retrograde axonal tracing studies (Newman and Ginsberg, 1994;Ruigrok, 2004;Fu et al, 2009). The main ventrocaudal portion of the lateral reticular nucleus is located within the ascending spinal fibers of the anterolateral funiculus.…”

Section: Lateral Reticular Nucleusmentioning

confidence: 99%