The Pre-S2 region of hepatitis b virus: More questions than answers

Gerber, Michael A.; Thung, Swan N.

doi:10.1002/hep.1840090227

Cited by 9 publications

(2 citation statements)

References 19 publications

(12 reference statements)

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“…This may depend either on the different types of vaccine used in the present study, and on the younger age of patients as well, in keeping with the trend of a better immune response to hepatitis B vaccine reported in the younger age groups. The alleged increased percentage of antibody responders-predicted among immunosuppressed individuals receiving vaccines that contain also Pre-S region proteins (possibly related to stronger immunogenicity) (Gerber, 1989;Miskovsky et al, 1991)-is not demonstrable in this series of DS children. In fact, the present authors found a good response both in patients vaccinated with Hevac B (putatively containing Pre-S proteins) and in those vaccinated with Engerix B (putatively free of Pre-S proteins) as well.…”

Section: Discussionmentioning

confidence: 66%

Vaccination against hepatitis B in preschool children with Down's syndrome

Vajro

Lettera

Fontanella

et al. 1992

J intellect Disabil Res

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Twenty-nine healthy HBsAg-and HBsAb-negative children with Down's syndrome who were living at home (mean age 42 months; 19 M, 10 F) were vaccinated against hepatitis B virus either with recombinant DNA or plasma-derived vaccine. Both groups of children responded well to the vaccination schedules, with HBsAb seroconversion rates close to 100%. Vaccination against hepatitis B in preschool children with Down's syndrome is effective in spite of the existing abnormalities of the immune function.

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Section: Discussionmentioning

confidence: 66%

Vaccination against hepatitis B in preschool children with Down's syndrome

Vajro

Lettera

Fontanella

et al. 1992

J intellect Disabil Res

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“…The distribution of pre-S polypeptides in the liver tissue and its relation to disease severity and HBV replicative activity is a focus of current research. 8 The intrahepatocytic accumulation of HBV encoded products, especially the surface antigen,"' has long been related to a "ground glass" appearance of whole or portion of hepatocyte cytoplasm, seen on haematoxylin and eosin stained liver sections, and more clearly shown by either orcein, aldehydefuchsin, or Victoria blue staining.…”

mentioning

confidence: 99%

Detection of hepatitis B virus pre-S1 and pre-S2 determinants in paraffin wax embedded liver tissue: importance of reagents used.

et al. 1991

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The presence of pre-S polypeptides in paraffin wax embedded liver sections from the biopsy specimens of 15 hepatitis B surface antigen (HBsAg) seropositive patients (five with chronic persistent hepatitis (CPH), four with chronic active hepatitis (CAH), four with cirrhosis and two "healthy" HBsAg carriers) was investigated using monoclonal antibodies directed to distinct epitopes on pre-Sl (18/7 and TO 606) and pre-S2 (5535 and Q 19/10). Pre

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Assay of hepatitis B virus DNA by polymerase chain reaction and its relationship to Pre-S- and S-encoded viral surface antigens

et al. 1991

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The polymerase chain reaction was evaluated as a diagnostic tool in 72 chronic hepatitis B virus carriers. Hepatitis B virus DNA was detectable in the serum of HBsAg-positive virus carriers using aliquots as small as 100 al. The detection limit for cloned hepatitis B virus DNA was 100 ag. Primer pairs for different regions of the HBV genome resulted in different sensitivity. Detection of the amplified hepatitis B virus DNA by Southern blotting and subsequent scintillation counting or densitometry allowed a semiquantitative assay. Using several primer pairs in parallel for optimal detection, all HBeAg-positive HBsAg carriers, 80% of HBe antibody-positive symptomatic HBsAg carriers and 57% of asymptomatic HBe antibody-positive HBsAg carriers were found to have hepatitis B virus DNA in the serum. During antiviral therapy hepatitis B virus DNA disappeared by the polymerase chain reaction assay in patients who became HBeAg negative, but polymerase chain reaction detected a relapse earlier than did the conventional dot blot. Pre-S antigens were assayed in serum and liver samples from most chronic carriers by enzyme-linked immunosorbent assay and/or immunoblot. Although most viremic carriers were strongly positive for pre-S1 and pre-S2 antigens, some hepatitis B virus DNA-positive HBsAg carriers did not have detectable pre-S antigens, and vice versa. Our data show that assay of hepatitis B virus DNA in the serum by polymerase chain reaction is by far more proficient than by dot blot and that it cannot be replaced by serological assays of HBeAg or pre-S antigen.

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The Pre-S2 region of hepatitis b virus: More questions than answers

Cited by 9 publications

References 19 publications

Vaccination against hepatitis B in preschool children with Down's syndrome

Vaccination against hepatitis B in preschool children with Down's syndrome

Detection of hepatitis B virus pre-S1 and pre-S2 determinants in paraffin wax embedded liver tissue: importance of reagents used.

Assay of hepatitis B virus DNA by polymerase chain reaction and its relationship to Pre-S- and S-encoded viral surface antigens

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