The power of neuropeptide precursor sequences to reveal phylogenetic relationships in insects: A case study on Blattodea

Bläser, Marcel; Misof, Bernhard; Predel, Reinhard

doi:10.1016/j.ympev.2019.106686

Cited by 9 publications

(9 citation statements)

References 41 publications

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“…We used these data to obtain the deduced amino acid sequences from 35 neuropeptide precursors per species. The suitability of neuropeptide precursor sequences for phylogenetic inferences was previously demonstrated in a proof-of-concept study ( Bläser, Misof & Predel, 2020 ). This approach is relatively fast and simple as it is based on a limited set of easily identifiable and well conserved protein coding genes.…”

Section: Introductionmentioning

confidence: 96%

Phylogenomics of darkling beetles (Coleoptera: Tenebrionidae) from the Atacama Desert

Ragionieri

Zúñiga-Reinoso

Bläser

et al. 2023

PeerJ

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Background Tenebrionidae (Insecta: Coleoptera) are a conspicuous component of desert fauna worldwide. In these ecosystems, they are significantly responsible for nutrient cycling and show remarkable morphological and physiological adaptations. Nevertheless, Tenebrionidae colonizing individual deserts have repeatedly emerged from different lineages. The goal of our study was to gain insights into the phylogenetic relationships of the tenebrionid genera from the Atacama Desert and how these taxa are related to the globally distributed Tenebrionidae. Methods We used newly generated transcriptome data (47 tribes, 7 of 11 subfamilies) that allowed for a comprehensive phylogenomic analysis of the tenebrionid fauna of this hyperarid desert and fills a gap in our knowledge of the highly diversified Tenebrionidae. We examined two independent data sets known to be suitable for phylogenomic reconstructions. One is based on 35 neuropeptide precursors, the other on 1,742 orthologous genes shared among Coleoptera. Results The majority of Atacama genera are placed into three groups, two of which belong to typical South American lineages within the Pimeliinae. While the data support the monophyly of the Physogasterini, Nycteliini and Scotobiini, this does not hold for the Atacama genera of Edrotini, Epitragini, Evaniosomini, Praociini, Stenosini, Thinobatini, and Trilobocarini. A suggested very close relationship of Psammetichus with the Mediterranean Leptoderis also could not be confirmed. We also provide hints regarding the phylogenetic relationships of the Caenocrypticini, which occur both in South America and southern Africa. Apart from the focus on the Tenebrionidae from the Atacama Desert, we found a striking synapomorphy grouping Alleculinae, Blaptinae, Diaperinae, Stenochinae, and several taxa of Tenebrioninae, but not Tenebrio and Tribolium. This character, an insertion in the myosuppressin gene, defines a higher-level monophyletic group within the Tenebrionidae. Conclusion Transcriptome data allow a comprehensive phylogenomic analysis of the tenebrionid fauna of the Atacama Desert, which represents one of the seven major endemic tribal areas in the world for Tenebrionidae. Most Atacama genera could be placed in three lineages typical of South America; monophyly is not supported for several tribes based on molecular data, suggesting that a detailed systematic revision of several groups is necessary.

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Section: Introductionmentioning

confidence: 96%

Phylogenomics of darkling beetles (Coleoptera: Tenebrionidae) from the Atacama Desert

Ragionieri

Zúñiga-Reinoso

Bläser

et al. 2023

PeerJ

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“…Orthology assessment and alignments were performed as described in Bläser et al (3). Briefly, we mined transcriptome sequences, provided by the 1KITE initiative (GenBank Umbrella BioProject ID PRJNA183205), for single-copy neuropeptide precursors in the datasets from each order of Polyneoptera; starting with neuropeptide precursor sequences of Carausius morosus (2), Locusta migratoria (1) and Blattodea (3). Once a full set of single-copy neuropeptide precursors was obtained, we used this information to search for precursors in the remaining species of this order or species from related orders.…”

Section: Orthology Assessment and Alignment Of Neuropeptide Precursormentioning

confidence: 99%

“…The sequences of single-copy neuropeptides, which are the focus of our study, are on average better conserved than those of multiple-copy peptides because amino acid (AA) substitutions in the single ligand of a particular neuropeptide receptor are potentially more likely to lead to a general loss of function than substitutions involving only one of several related neuropeptides produced from the same precursor. Thus, mutations that alter the sequences of singlecopy neuropeptides must either be accompanied by parallel mutations in receptor genes that maintain the binding properties of the respective receptors or should not alter the steric properties of the peptides to maintain functionality (3). Most neuropeptides activate peptide-specific G-protein coupled receptors and many of these peptidergic systems have a very ancient origin that can be traced back to the early evolution of the Metazoa [e.g., (4)].…”

Section: Introductionmentioning

confidence: 99%

“…Such data have been used in several comprehensive studies, mainly to compile neuropeptide precursor sequences within higher taxa (23)(24)(25). In a recent study a large dataset of neuropeptide precursors from Blattodea was used to demonstrate the considerable phylogenetic information contained in these sequences (3). A particular focus on the general evolution of neuropeptide precursors has been placed in a study on the precursors of 12 Drosophila species (26).…”

Section: Introductionmentioning

confidence: 99%

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Evolution of Neuropeptide Precursors in Polyneoptera (Insecta)

Bläser

Predel

2020

Front. Endocrinol.

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Neuropeptides are among the structurally most diverse signaling molecules and participate in intercellular information transfer from neurotransmission to intrinsic or extrinsic neuromodulation. Many of the peptidergic systems have a very ancient origin that can be traced back to the early evolution of the Metazoa. In recent years, new insights into the evolution of these peptidergic systems resulted from the increasing availability of genome and transcriptome data which facilitated the investigation of the complete neuropeptide precursor sequences. Here we used a comprehensive transcriptome dataset of about 200 species from the 1KITE initiative to study the evolution of single-copy neuropeptide precursors in Polyneoptera. This group comprises well-known orders such as cockroaches, termites, locusts, and stick insects. Due to their phylogenetic position within the insects and the large number of old lineages, these insects are ideal candidates for studying the evolution of insect neuropeptides and their precursors. Our analyses include the orthologs of 21 single-copy neuropeptide precursors, namely ACP, allatotropin, AST-CC, AST-CCC, CCAP, CCHamide-1 and 2, CNMamide, corazonin, CRF-DH, CT-DH, elevenin, HanSolin, NPF-1 and 2, MS, proctolin, RFLamide, SIFamide, sNPF, and trissin. Based on the sequences obtained, the degree of sequence conservation between and within the different polyneopteran lineages is discussed. Furthermore, the data are used to postulate the individual neuropeptide sequences that were present at the time of the insect emergence more than 400 million years ago. The data confirm that the extent of sequence conservation across Polyneoptera is remarkably different between the different neuropeptides. Furthermore, the average evolutionary distance for the single-copy neuropeptides differs significantly between the polyneopteran orders. Nonetheless, the single-copy neuropeptide precursors of the Polyneoptera show a relatively high degree of sequence conservation. Basic features of these precursors in this very heterogeneous insect group are explained here in detail for the first time.

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“…In recent years, the study of the neuropeptides has been expanded in several insect orders like Diptera, Lepidoptera, Blattodea, Coleoptera, Hemiptera, and Hymenoptera ( Bläser et al., 2020 ; Chang et al., 2018 ; Llopis-Giménez et al., 2019 ; Ragionieri and Predel, 2020 ; Riehle et al., 2002 ; Roller et al., 2008 ; Traverso et al., 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Prediction of neuropeptide precursors and differential expression of adipokinetic hormone/corazonin-related peptide, hugin and corazonin in the brain of malaria vector Nyssorhynchus albimanus during a Plasmodium berghei infection

Alvarado-Delgado

Martínez-Barnetche

Téllez-Sosa

et al. 2021

Current Research in Insect Science

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The power of neuropeptide precursor sequences to reveal phylogenetic relationships in insects: A case study on Blattodea

Cited by 9 publications

References 41 publications

Phylogenomics of darkling beetles (Coleoptera: Tenebrionidae) from the Atacama Desert

Phylogenomics of darkling beetles (Coleoptera: Tenebrionidae) from the Atacama Desert

Evolution of Neuropeptide Precursors in Polyneoptera (Insecta)

Prediction of neuropeptide precursors and differential expression of adipokinetic hormone/corazonin-related peptide, hugin and corazonin in the brain of malaria vector Nyssorhynchus albimanus during a Plasmodium berghei infection

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