The powdery mildew resistance gene <i>Pm8</i> derived from rye is suppressed by its wheat ortholog <i>Pm3</i>

Hurni, Severine; Brunner, Susanne; Stirnweis, Daniel; Herren, Gerhard; Peditto, David; McIntosh, R. A.; Keller, Beat

doi:10.1111/tpj.12593

Cited by 128 publications

(89 citation statements)

References 37 publications

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“…Because Sr33 and Sr50 are derived from diploid wheat and rye, respectively (10,11), these genes do not naturally occur together in the same plant, but both have been introgressed into cultivated wheat to provide stem rust resistance. Their hetero-association in planta raises the possibility that they could interfere functionally, because such interference has been reported for the powdery mildew resistance gene Pm8, derived from rye, and its wheat ortholog Pm3, which also form heterocomplexes (27).…”

Section: Discussionmentioning

confidence: 99%

Cytosolic activation of cell death and stem rust resistance by cereal MLA-family CC–NLR proteins

Césari¹,

Moore²,

Chen³

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

105

120

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Plants possess intracellular immune receptors designated “nucleotide-binding domain and leucine-rich repeat” (NLR) proteins that translate pathogen-specific recognition into disease-resistance signaling. The wheat immune receptors Sr33 and Sr50 belong to the class of coiled-coil (CC) NLRs. They confer resistance against a broad spectrum of field isolates of Puccinia graminis f. sp. tritici, including the Ug99 lineage, and are homologs of the barley powdery mildew-resistance protein MLA10. Here, we show that, similarly to MLA10, the Sr33 and Sr50 CC domains are sufficient to induce cell death in Nicotiana benthamiana. Autoactive CC domains and full-length Sr33 and Sr50 proteins self-associate in planta. In contrast, truncated CC domains equivalent in size to an MLA10 fragment for which a crystal structure was previously determined fail to induce cell death and do not self-associate. Mutations in the truncated region also abolish self-association and cell-death signaling. Analysis of Sr33 and Sr50 CC domains fused to YFP and either nuclear localization or nuclear export signals in N. benthamiana showed that cell-death induction occurs in the cytosol. In stable transgenic wheat plants, full-length Sr33 proteins targeted to the cytosol provided rust resistance, whereas nuclear-targeted Sr33 was not functional. These data are consistent with CC-mediated induction of both cell-death signaling and stem rust resistance in the cytosolic compartment, whereas previous research had suggested that MLA10-mediated cell-death and disease resistance signaling occur independently, in the cytosol and nucleus, respectively.

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Section: Discussionmentioning

confidence: 99%

Cytosolic activation of cell death and stem rust resistance by cereal MLA-family CC–NLR proteins

Césari¹,

Moore²,

Chen³

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

105

120

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“…Recently, a few articles were studied to identify QTLs related to heat tolerance (Xiao et al 2011;Cao et al 2013;Zhang et al 2014). These QTLs related to heat tolerance were used to develop the promising lines of heat tolerance based on marker-assisted selection (MAS) (Xu and Couch 2008, Avise 2004, Hurni et al 2014. A variety OM8608 genotype, which was selected for QTL related to heat tolerance by MAS is well adaptable to Ninh Thuan Province of hot spot for drought and heat stress in Vietnam.…”

Section: Discussionmentioning

confidence: 99%

“…Many reviews comparing the molecular genetic issues related to different types of markers assay, which can significantly affect the success of MAS (Avise 2004). Marker assisted introgression has been shown as an effective approach for precise transfer of genes from wild species with minimum linkage drag and also it could help in identifying genotypes containing the target gene in early generations even if it is suppressed in a particular genetic background (Hurni et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Breeding for Heat Tolerance Rice Based on Marker-Assisted Backcrosing in Vietnam

Lang¹,

Ha²,

Tru³

et al. 2015

Plant Breed. Biotech.

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This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.Plant Breed. Biotech. 2015 (September) ABSTRACT A total of six markers RM3586 and RM160 on chromosome 3 and RM3735, RM3471, RM3687 and RM3536 on chromosome 4 were used to select promising lines in backcrossing populations for heat tolerance at flowering stage in rice. Fifty lines selected in BC3F2, BC4F1, and BC4F2 and parents were planted in 2013, and 2014 dry seasons at the CLRRI field under natural heat stress and greenhouse to evaluate heat tolerance at the reproductive period. Heat tolerance scoring under field condition was based on percentage of unfilled grains. All selected lines exhibited their homozygous alleles with two heat tolerance germplasm N22 or Dular in QTL loci. Twelve lines harboring homozygous alleles to QTL loci RM3586 on chromosome 3 and RM3735 on chromosome 4, respectively were selected and evaluated to agronomic traits and yield potential. Four lines BC4-1-10-1 from OM5930/N22//4 *OM5930, BC4-5-8 from OM5930/Dular//4*OM5930, BC4-5-9-4 from AS996/N22//4*AS996, and BC4-6-3 from AS996/Dular//4 *AS996, respectively were finally selected to would be for regional adaptable test in Central Coast of Vietnam under heat stress condition to release to rice farmers.

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“…Изучение супрессии у 1R(1B) замещенных и 1RS.1BL транслоциро ванных линий указывает на то, что в качестве генасупрес сора выступает Pm3, локализованный в хромосоме 1AS. При анализе коэкспрессии генов Pm3 и Pm8 в трансгенных растениях табака установлено, что механизм супрессии может заключаться в посттрансляционных взаимодей ствиях белковых продуктов этих генов, которые препят ствуют передаче сигнала для проявления защитной реак ции генов (Hurni et al, 2014).…”

Section: влияние генотипической среды на проявление признаков устойчиunclassified

INLUENCE OF ALIEN GENETIC MATERIAL ON THE MANIFESTATION OF AGRONOMICALLY IMPORTANT TRAITS OF COMMON WHEAT (T. aestivum L.)

Леонова¹

2018

Vestn. VOGiS

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The powdery mildew resistance gene Pm8 derived from rye is suppressed by its wheat ortholog Pm3

Cited by 128 publications

References 37 publications

Cytosolic activation of cell death and stem rust resistance by cereal MLA-family CC–NLR proteins

Cytosolic activation of cell death and stem rust resistance by cereal MLA-family CC–NLR proteins

Breeding for Heat Tolerance Rice Based on Marker-Assisted Backcrosing in Vietnam

INLUENCE OF ALIEN GENETIC MATERIAL ON THE MANIFESTATION OF AGRONOMICALLY IMPORTANT TRAITS OF COMMON WHEAT (T. aestivum L.)

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