The Potentiometric Urea Biosensor Using Chitosan Membrane

Mulyasuryani, Ani; Roosdiana, Anna; Srihardyastutie, Arie

doi:10.22146/ijc.21454

Cited by 12 publications

(7 citation statements)

References 1 publication

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The conductivity is measured at pH 9, based on previous work result. 5 The optimum performance biosensor is resulted at a 10 μm of membrane thickness (Fig. 2).…”

Section: Resultsmentioning

confidence: 98%

“…3 The potentiometric uric acid biosensor has been developed. 4,5 The biosensor is based on the oxidation of uric acid calatyzed by uricase to produce CO 2 . In water, CO 2 can be hidrolyzed to form HCO 3 – and H 3 O + .…”

Section: Introductionmentioning

confidence: 99%

“…However, the biosensor needs large sample volume. 5 In this work, we developed a coductimetric uric acid biosensor. Uricase is immobilized on a nata de coco membrane—Pt electrode, the biosensor has a simple design and is smaller than the potentiometric biosensor.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Conductimetric Biosensor for the Detection of Uric Acid by Immobilization Uricase on Nata de Coco Membrane—Pt Electrode

Mulyasuryani

Srihardiastutie

2011

Anal Chem�Insights

Self Cite

View full text Add to dashboard Cite

A conductimetric enzyme biosensor for uric acid detection has been developed. The uricase, as enzyme, is isolated from Candida utilis and immobilized on a nata de coco membrane-Pt electrode. The biosensor demonstrates a linear response to urate over the concentration range 1–6 ppm and has good selectivity properties. The response is affected by the membrane thickness and pH change in the range 7.5–9.5. The response time is three minutes in aqueous solutions and in human serum samples. Application of the biosensor to the determination of uric acid in human serum gave results that compared favourably with those obtained by medical laboratory. The operational stability of the biosensor was not less than three days and the relative error is smaller than 10%.

show abstract

“…The conductivity is measured at pH 9, based on previous work result. 5 The optimum performance biosensor is resulted at a 10 μm of membrane thickness (Fig. 2).…”

Section: Resultsmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Conductimetric Biosensor for the Detection of Uric Acid by Immobilization Uricase on Nata de Coco Membrane—Pt Electrode

Mulyasuryani

Srihardiastutie

2011

Anal Chem�Insights

Self Cite

View full text Add to dashboard Cite

show abstract

“…The nanocomposites exhibited excellent conductivity, catalytic activity, and biocompatibility and the polymers improved the biosensor stability. Potentiometric urea biosensor with the enzyme urease resulting to the CO 2 formation was constructed using chitosan membrane and the pH electrode as a transducer (Mulyasuryani et al 2010). Chitosan membrane with amino groups facilitates interaction with the acid like CO 2 .…”

Section: Membranes For the Immobilization Of Biocomponents And Electrmentioning

confidence: 99%

Protective membranes at electrochemical biosensors

Ambrozy¹,

Hlavata²,

Labuda³

2013

Acta Chimica Slovaca

View full text Add to dashboard Cite

The primary role of the biosensor is to specifically detect an analyte using biochemical reaction or interaction mediated by isolated biomolecules, organelles, whole cells or biomimetic receptors. In terms of construction and function, the biosensor consists of biorecognition element and transducer connected to suitable measurement device. Electrochemical biosensor is an electrode or microelectrode with the surface chemically modified by the biorecognition element. The main problem of analysis with the biosensors is the presence of low and high molecular weight substances in the sample that interfere at the detection of analyte. Due to deposition of surface active compounds the biosensor response may be diminished depending on time of interaction with sample. These effects can be eliminated by using anti-interference membranes. This review deals with preparation and utilization of membranes for the biocomponent immobilization and with outer-sphere protective membranes.

show abstract

“…Chitosan is the most abundant cationic polymer widely used for biomedical and pharmaceutical applications. Chitosan has the ability to immobilize the enzymes (Yang et al, 2013;Mulyasuryani et al, 2010) and to entrap the DNA molecules (Galandova and Labuda, 2009). Nafion is a polymer with cation exchange properties.…”

Section: Introductionmentioning

confidence: 99%

DNA-based biosensors with polyvinyl alcohol external membrane as a tool for the evaluation of antioxidant activity of white wines

Svitková

Steffelová

Blaškovičová

et al. 2015

Acta Chimica Slovaca

View full text Add to dashboard Cite

Electrochemical DNA-based biosensors with external protective membranes were prepared for the evaluation of antioxidant properties of white wines against pro-oxidant hydroxyl radicals. A glassy carbon working electrode (GCE) was modified using a layer-by-layer deposition technique with low molecular weight double stranded DNA and an outer sphere polymer film membrane of Nafion, chitosan or polyvinylalcohol. The composition of a working procedure with membrane-covered DNA biosensors were optimized with respect to their voltammetric response in solution of the DNA redox indicator [Fe(CN)6]3-/4-after the incubation in white wines. Polyvinylalcohol (PVA) film was proved to be the suitable membrane. The PVA/DNA/GCE biosensor was used for the detection of a deep degradation of the surface-attached DNA at the incubation in the cleavage agent and for the evaluation of antioxidant properties of white wines at the incubation in mixtures of cleavage agent and wine. The investigation of the biosensors with protective membranes represents a significant contribution to utilization of the electrochemical DNA based biosensors for practical purposes.

show abstract

The Potentiometric Urea Biosensor Using Chitosan Membrane

Cited by 12 publications

References 1 publication

Conductimetric Biosensor for the Detection of Uric Acid by Immobilization Uricase on Nata de Coco Membrane—Pt Electrode

Conductimetric Biosensor for the Detection of Uric Acid by Immobilization Uricase on Nata de Coco Membrane—Pt Electrode

Protective membranes at electrochemical biosensors

DNA-based biosensors with polyvinyl alcohol external membrane as a tool for the evaluation of antioxidant activity of white wines

Contact Info

Product

Resources

About