The potentiation of beta-lactam and anti-bacterial activities of lipophilic constituents from Mesua ferrae leaves against methicillin-resistant Staphylococcus aureus

Abstract

ObjectivesMesua ferrae, from the family of Calophyllaceae, is traditionally used for the treatment of piles, fever and renal disorders. The present study was aimed to examine the antibacterial compounds from the leaves of M. ferrae and their β-lactam antibiotic potentiate activities against Staphylococcus aureus and methicillin-resistant Staphylococcus aureus (MRSA).MethodsStigmasterol (1) and β-caryophyllene oxide (2) were isolated from the n-hexane fraction of the leaves of M. ferrae using a bioassay-guided fractionation approach.ResultsThe isolated compounds displayed anti-Staphylococcus and anti-MRSA activities. It is worth to note that both compounds demonstrated synergism with β-lactam antibiotics against S. aureus and MRSA. Gas chromatography–mass spectrometry (GC–MS) analysis indicated the n-hexane fraction was dominated by triterpenes and sesquiterpenes, suggesting the total antibacterial activity exhibited by the fraction.ConclusionBased on the findings, it could conclude that M. ferrae is a promising natural source for the discovery of new anti-MRSA lead compounds.

Essential Oils of Alpinia monopleura and Their Antibacterial and Antioxidant Activity

“…Inhibition of the growth of E. coli and S. aureus bacteria in limonene showed MIC values of 16 μL/mL (Gupta et al, 2021) and 20 μL/mL (Han et al, 2021), β -caryophyllene, respectively, showed MIC values of 9 μM and 3 μM, respectively (Dahham et al, 2015). Caryophyllene oxide showed MIC values of 60 μg/mL (Schmidt et al, 2010) and 15.63 μg/mL (Alagasamy et al, 2021).…”