The potentials of secondary metabolites from Bacillus cereus SN7 and Vagococcus fluvialis CT21 against fish pathogenic bacteria

Feliatra, F.; Batubara, Ummi Mardhiah; Nurulita, Yuana; Lukistyowati, Iesje; Setiaji, Jarot

doi:10.1016/j.micpath.2021.105062

“…Enterococcus faecalis has been described colonizing the surface of nests of Melipona quadrifasciata and the species Alcaligenes faecalis is a fecal coliform found in the species Trigona spinipes [ 48 ]. Secondary metabolites of Vagococcus fluvials have been described as inhibiting the growth of bacteria such as Pseudomonas aeruginosa , Vibrio alginolyticus , and Aeromonas hydrophila [ 49 ], as well as in our study, where they inhibited the growth of multiresistant P. aeruginosa and KPC. Indicating that these organisms have great potential for discovering new molecules with antibiotic activity.…”

Section: Discussionsupporting

confidence: 81%

Antimicrobial activity of supernatants produced by bacteria isolated from Brazilian stingless bee’s larval food

Santos

¹

,

Malta

²

,

Dantas

³

et al. 2022

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Background The discovery of new molecules with antimicrobial properties has been a promising approach, mainly when related to substances produced by bacteria. The use of substances produced by bees has evidenced the antimicrobial action in different types of organisms. Thus, the use of bacteria isolated from larval food of stingless bees opens the way for the identification of the new molecules. The effect of supernatants produced by these bacteria was evaluated for their ability to inhibit the growth of bacteria of clinical interest. Furthermore, their effects were evaluated when used in synergy with antibiotics available in the pharmaceutical industry. Results A few supernatants showed an inhibitory effect against susceptible and multiresistant strains in the PIC assay and the modulation assay. Emphasizing the inhibitory effect on multidrug-resistant strains, 7 showed an effect on multidrug-resistant Escherichia coli (APEC), Klebsiella pneumoniae carbapenemase (KPC), multidrug-resistant Pseudomonas aeruginosa, and multidrug-resistant Staphylococcus aureus (MRSA) in the PIC assay. Of the supernatants analyzed, some presented synergism for more than one species of multidrug-resistant bacteria. Nine had a synergistic effect with ampicillin on E. coli (APEC) or S. aureus (MRSA), 5 with penicillin G on E. coli (APEC) or KPC, and 3 with vancomycin on KPC. Conclusion In summary, the results indicate that supernatants produced from microorganisms can synthesize different classes of molecules with potent antibiotic activity against multiresistant bacteria. Thus, suggesting the use of these microorganisms for use clinical tests to isolate the molecules produced and their potential for use.

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“…Bacillus has the facility to secrete digestive enzymes and degrade macronutrients, which acts to boost intestinal digestibility in fish [ 68 , 69 ]. Meanwhile, Bacillus can inhibit the growth and reproduction of pathogenic bacteria [ 70 ]. Staphylococcus and Enterococcus are two pathogenic bacteria that can cause an inflammatory response [ 71 , 72 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Clostridium butyricum on Growth Performance, Intestinal Health, and Disease Resistance of Hybrid Grouper (Epinephelus fuscoguttatus $♀$ × E. lanceolatus $♂$ ) Fed with Cottonseed Protein Concentrate (CPC) Replacement of Fishmeal

Pan

¹

,

Li

²

,

Xie³

et al. 2023

Aquaculture Nutrition

1

0

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An 8-week feeding trial was conducted to investigate the effects of C. butyricum on the growth performance, microbiota, immunity response, and disease resistance in hybrid grouper fed with cottonseed protein concentrate (CPC) replacement of fishmeal. Six groups of isonitrogenous and isolipid diets were formulated including a positive control group (50% fishmeal, PC), a negative control group (CPC replaced 50% of fishmeal protein, NC), and Clostridium butyricum supplemented with 0.05% (C1, 5 × 10 8 CFU/kg), 0.2% (C2, 2 × 10 9 CFU/kg), 0.8% (C3, 8 × 10 9 CFU/kg), and 3.2% (C4, 3.2 × 10 10 CFU/kg), respectively, to the NC group. The results showed that weight gain rate and specific growth rate were significantly higher in the C4 group than that in the NC group ( P < 0.05 ). After supplementation with C. butyricum, the amylase, lipase, and trypsin activities were significantly higher than the NC group ( P < 0.05 ; except group C1), and the same results were obtained for intestinal morphometry. The intestinal proinflammatory factors were significantly downregulated, and the anti-inflammatory factors were significantly upregulated in the C3 and C4 groups compared with the NC group after supplementation with 0.8%-3.2% C. butyricum ( P < 0.05 ). At the phylum level, the PC, NC, and C4 groups were dominated by the Firmicutes and the Proteobacteria. At the genus level, the relative abundance of Bacillus in the NC group was lower than that in the PC and C4 groups. After supplementation with C. butyricum, grouper in the C4 group showed significantly higher resistance to V. harveyi than the NC group ( P < 0.05 ). Above all, taking into account the effects of immunity and disease resistance, it was recommended to supplement 3.2% C. butyricum in the diet of grouper fed the replacement of 50% fishmeal protein by CPC.

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“…The other mechanism of bacteria to survive is producing bacteriocin, from group of Bacillus sp. (Motta et al 2008;Sumi et al 2015) also producing secondary metabolites such as alkaloids, flavonoids, and saponins that are capable of inhibit the growth and reproduction of pathogens (Feliatra et al 2021). Possibly one or several combinations of these mechanisms was responsible for these results.…”

Section: Antibacterial Activitymentioning

confidence: 99%

“…Possibly one or several combinations of these mechanisms was responsible for these results. Several studies showed that B. cereus is capable of inhibiting V. alginolyticus (Feliatra et al 2021;Setiaji et al 2020), by producing secondary metabolites compounds i.e., alkaloid, flavonoid, and saponin (Feliatra et al 2021). These secondary metabolites compounds are capable to disrupt the peptidoglycan, one of the major constituents of bacterial cell wall, that causes lysis in cells (Feliatra et al 2021).…”

Section: Antibacterial Activitymentioning

confidence: 99%

Screening of sponge-associated bacteria to control vibriosis in vannamei shrimp (Litopenaeus vannamei)

Sarjito

¹

,

Amalia

²

,

Ayuningrum

³

2022

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Abstract. Sarjito S, Amalia R, Sabdaningsih A. 2022. Screening of sponge-associated bacteria to control vibriosis in vannamei shrimp (Litopenaeus vannamei). Biodiversitas 23: 5333-5341. An intensive shrimp culture with a high stocking density causes a higher chance of vannamei shrimp being infected with diseases. The most frequent disease is caused by Vibrio sp. Vibrio might cause a severe production loss in the shrimp culture, leading to larvae and mature stadium mortality at 50%. Therefore, a study on the alternative management of vibriosis disease using biocontrol agents is urgently needed. The sponge is a marine invertebrate that provides as a shelter for microbes to live and symbiosis with the animal. An evident ability of sponge-associated bacteria to produce antimicrobial compounds can be exploited as a biodegradable bio-agent, potentially as a solution to control vibriosis disease in a shrimp. This research aimed to isolate sponge-associated bacteria with vibriosis antibacterial potential. Sampling was done in Tulamben, Bali and Panjang island, Jepara, Indonesia with six sampling sites consisting of 15 sampling points. Moreover, vibriosis bacteria were provided by the laboratory of fish disease at Balai Besar Perikanan Budidaya Air Payau (BBPBAP) Jepara. Totally, 24 and 47 pure cultures were successfully isolated using Zobell 2216E agar medium from Tulamben, Bali and Panjang Island, Jepara respectively. The isolates were tested for antibacterial assay using plug agar diffusion methods. The top eight potential isolates were found in isolate B6.3C, B6.3D, B6.4E, B9.3C, PA.1, PA.4, PC.3, and PH.1 against Vibrio vulnificus, V. anguillarum, V. alginolyticus, V. parahaemolyticus, however there was no activity in V. harveyi. The molecular method was carried out to identify those potential isolates using universal primer 16S rRNA. The BLAST homology showed that all potential antibacterial isolates belong to the family Bacilllaceae, such as Bacillus cereus, Virgibacillus salarius, B. aerius, B. paramycoides, B. thuringiensis, and B. altitudinis. Further research will be carried out on microencapsulation and in vivo tests.

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The potentials of secondary metabolites from Bacillus cereus SN7 and Vagococcus fluvialis CT21 against fish pathogenic bacteria

Cited by 14 publications

References 37 publications

Antimicrobial activity of supernatants produced by bacteria isolated from Brazilian stingless bee’s larval food

Antimicrobial activity of supernatants produced by bacteria isolated from Brazilian stingless bee’s larval food

Effects of Clostridium butyricum on Growth Performance, Intestinal Health, and Disease Resistance of Hybrid Grouper (Epinephelus fuscoguttatus $♀$ × E. lanceolatus $♂$ ) Fed with Cottonseed Protein Concentrate (CPC) Replacement of Fishmeal

Screening of sponge-associated bacteria to control vibriosis in vannamei shrimp (Litopenaeus vannamei)

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The potentials of secondary metabolites from Bacillus cereus SN7 and Vagococcus fluvialis CT21 against fish pathogenic bacteria

Cited by 14 publications

References 37 publications

Antimicrobial activity of supernatants produced by bacteria isolated from Brazilian stingless bee’s larval food

Antimicrobial activity of supernatants produced by bacteria isolated from Brazilian stingless bee’s larval food

Effects of Clostridium butyricum on Growth Performance, Intestinal Health, and Disease Resistance of Hybrid Grouper (Epinephelus fuscoguttatus ♀ × E. lanceolatus ♂ ) Fed with Cottonseed Protein Concentrate (CPC) Replacement of Fishmeal

Screening of sponge-associated bacteria to control vibriosis in vannamei shrimp (Litopenaeus vannamei)

Contact Info

Product

Resources

About

Effects of Clostridium butyricum on Growth Performance, Intestinal Health, and Disease Resistance of Hybrid Grouper (Epinephelus fuscoguttatus $♀$ × E. lanceolatus $♂$ ) Fed with Cottonseed Protein Concentrate (CPC) Replacement of Fishmeal