The Potentiality of Human Umbilical Cord Isolated Mesenchymal Stem/Stromal Cells for Cardiomyocyte Generation [Corrigendum]

Abou-ElNaga, Amoura; El-Chennawi, Farha; kamel, Samar Ibrahim; Mutawa, Ghada

doi:10.2147/sccaa.s300939

Cited by 4 publications

(4 citation statements)

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“…To obtain good outcomes in stem cell therapy, it is critical to prepare stem cells with a high therapeutic potential for transplantation. The synergic effect of 5-Aza and bFGF in MSCs confirmed through a high expression of TNNT1, Desmin, and NKX2.5 as the cardiac-specific biomarkers [ 32 ].…”

Section: Discussionmentioning

confidence: 99%

Greater angiogenic and immunoregulatory potency of bFGF and 5-aza-2ʹ-deoxycytidine pre-treated menstrual blood stem cells in compare to bone marrow stem cells in rat model of myocardial infarction

Manshori

Kazemnejad

Naderi

et al. 2022

BMC Cardiovasc Disord

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Background This study is designed to compare the menstrual blood stem cells (MenSCs) and bone marrow stem cells (BMSCs)-secreted factors with or without pre-treatment regimen using basic fibroblast growth factor (bFGF) and 5-aza-2ʹ-deoxycytidine (5-aza) and also regenerative capacity of pre-treated MenSCs and/or BMSCs in a rat model of myocardial infarction (MI). Methods BMSCs and MenSCs were pre-treated with bFGF and 5-aza for 48 h and we compared the paracrine activity by western blotting. Furthermore, MI model was created and the animals were divided into sham, MI, pre-treated BMSCs, and pre-treated MenSCs groups. The stem cells were administrated via tail vain. 35 days post-MI, serum and tissue were harvested for further investigations. Results Following pre-treatment, vascular endothelium growth factor, hypoxia-inducible factor-1, stromal cell-derived factor-1, and hepatocyte growth factor were significantly increased in secretome of MenSCs in compared to BMSCs. Moreover, systemic administration of pre-treated MenSCs, leaded to improvement of cardiac function, preservation of myocardium from further subsequent injuries, promotion the angiogenesis, and reduction the level of NF-κB expression in compared to the pre-treated BMSCs. Also, pre-treated MenSCs administration significantly decreased the serum level of Interleukin 1 beta (IL-1β) in compared to the pre-treated BMSCs and MI groups. Conclusions bFGF and 5-aza pre-treated MenSCs offer superior cardioprotection compare to bFGF and 5-aza pre-treated BMSCs following MI.

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Section: Discussionmentioning

confidence: 99%

Greater angiogenic and immunoregulatory potency of bFGF and 5-aza-2ʹ-deoxycytidine pre-treated menstrual blood stem cells in compare to bone marrow stem cells in rat model of myocardial infarction

Manshori

Kazemnejad

Naderi

et al. 2022

BMC Cardiovasc Disord

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“…5-AZA, widely used in the cardiac differentiation of stem cells, can regulate the histone demethylation and DNA methylation (20). A number of stem cells have been used to differentiate into cardiomyocytes by 5-AZA (19)(20)(21)(22)(23). The mechanism of 5-AZA may be related to CpG base-pair demethylation and regulation of early myocardial transcription factors (24)(25)(26)(27).…”

Section: Discussionmentioning

confidence: 99%

H3K9me2 regulates early transcription factors to promote mesenchymal stem‑cell differentiation into cardiomyocytes

Sun

et al. 2021

Mol Med Rep

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Studies have shown that histone H3 at lysine 9 (H3K9me2) is an important epigenetic modifier of embryonic development, cell reprogramming and cell differentiation, but its specific role in cardiomyocyte formation remains to be elucidated. The present study established a model of 5-Azacytidine-induced differentiation of rat bone mesenchymal stem cells (MSCs) into cardiomyocytes and, on this basis, investigated the dimethylation of H3K9me2 and its effect on cardiomyocyte formation by knockdown of H3K9me2 methylase, euchromatic histone-lysine N-methyltransferase 2 (G9a) and H3K9me2 lysine demethylase 3A (KDM3A). The results demonstrated that, in comparison with the normal induction process, the knockdown of G9a could significantly reduce the H3K9me2 level of the MSCs in the induced model. Reverse transcription-quantitative (RT-q) PCR demonstrated that the expression of cardiac troponin T(cTnT) was significantly increased. In addition, flow cytometry demonstrated that the proportion of cTnT-positive cells was significantly increased on day 21. With the knockdown of KDM3A, the opposite occurred. In order to explore the specific way of H3K9me2 regulating cardiomyocyte formation, western blotting and RT-qPCR were used to detect the expression of key transcription factors including GATA binding protein 4 (GATA-4), NK2 Homeobox 5 (Nkx2.5) and myocyte enhancer factor 2c (MEF2c) during cardiomyocyte formation. The decrease of H3K9me2 increased the expression of transcription factors GATA-4, Nkx2.5 and MEF2c in the early stage of myocardial development while the increase of H3K9me2 inhibited the expression of those transcription factors. Accordingly, it was concluded that H3K9me2 is a negative regulator of cardiomyocyte formation and can participate in cardiomyocyte formation by activating or inhibiting key transcription factors of cardiomyocytes, which will lay the foundation for the optimized induction efficiency of cardiomyocytes in in vitro and clinical applications.

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“…In addition, umbilical cord HSCs have a competitive advantage over bone marrow hematopoietic stem cells because they graft 10-50 times better on host xenogenic. Still, their percentage among mononuclear cells decreases with gestational age, from 11% at 17 weeks to 1% at 38 weeks (16).…”

Section: Introductionmentioning

confidence: 92%

Molecular Process of Stem Cells in Modern Biology

Shahid¹,

Rehman²,

Hyder³

et al. 2022

PJMHS

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Stem cells have a wide range of traits, including the ability to self-renewal and differential interactions between various cell types that vary with their location within tissue and their immediate surroundings. Inhibitors of both are influenced by the cell cycle, genes involved in chromosomal rearrangements, critical developmental proteins, and signaling pathways. Wnt, Notch, Hedgehog Pathways, BMI-1, OCT3/4, ARF, NANOG, p16Ink4a, and this includes HOXB4, SOX2, and their homologous paralog, govern self-renewal. Stem cells and their molecular pathways that regulate self-renewal and differentiation hold great promise for theoretical and practical studies. Keyword: gene regulation of stem cell self-renewal and differentiation genes for BMI-1, NANOG, OCT3, OCT4, p16Ink4a, SOX2, microenvironment, ARF protein proteins 1 (hedgehog), proteins 2 (Wnt.), and receptors 3 (notch).

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The Potentiality of Human Umbilical Cord Isolated Mesenchymal Stem/Stromal Cells for Cardiomyocyte Generation [Corrigendum]

Cited by 4 publications

References 0 publications

Greater angiogenic and immunoregulatory potency of bFGF and 5-aza-2ʹ-deoxycytidine pre-treated menstrual blood stem cells in compare to bone marrow stem cells in rat model of myocardial infarction

Greater angiogenic and immunoregulatory potency of bFGF and 5-aza-2ʹ-deoxycytidine pre-treated menstrual blood stem cells in compare to bone marrow stem cells in rat model of myocardial infarction

H3K9me2 regulates early transcription factors to promote mesenchymal stem‑cell differentiation into cardiomyocytes

Molecular Process of Stem Cells in Modern Biology

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