The potential value of microRNA-4463 in the prognosis evaluation in hepatocellular carcinoma

Abstract

The purpose of this study is to measure the expression of microRNA-4463 and microRNA-6087 between normal persons and patients with hepatocellular carcinoma (HCC), and to clarify the meaning of them in the prognosis evaluation in HCC. Forty-five samples from healthy people and patients, who had been diagnosed with hepatocellular carcinoma before any treatment, were collected to study respectively. Real-time PCR was used to detect the expression of miRNA-4463 and miRNA-6087 in the serum of control group and hepatocellular carcinoma patients. The expression of miR-4463 in the serum of HCC patients was significantly higher than that in control group (P < 0.05), and the expression level was independent of gender, tumor size, cell types, stages, alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and HBsAg status (P > 0.05). But there was a significant difference of different level of AFP in HCC (P < 0.05), and the difference between the group of AFP lower than 400 ug/l and the control group is statistically significant (P < 0.05). Besides, the survival time had showed a significant difference at the high and low expression levels (P < 0.05). But the expression level of miRNA-6087 was no difference in HCC and control group. The disorder of miRNA-4463 occurred in HCC, even the AFP level doesn't rises. What's more, patients who get the high level of miRNA-4463 seem to have a shorter survival time. And it contributes great to the prognostic evaluation. This is the first study to illustrate the potential significance of miRNA-4463 in the prognosis in HCC.

Prognostic value of microRNAs in hepatocellular carcinoma: a meta-analysis

“…Numerous articles reported that dysregulated expression levels of miRNAs correlated with survival time of HCC patients [ 1 – 221 ]. Nevertheless, there has not been a comprehensive meta-analysis to assess the accurate prognostic value of miRNAs in HCC.…”

“…Numerous studies reported expression levels of tissue [ 1 – 194 ] or blood [ 195 – 221 ] miRNAs were related with prognosis of HCC patients. HCC is one of the most common tumors, over 700,000 new cases are reported yearly, and HCC is considered as the third primary etiology of tumor-associated mortality rate globally [ 222 – 224 ].…”

Biomarkers vs imaging in the early detection of hepatocellular carcinoma and prognosis

“…Liu et al[59] reported miRNA-125b, AFP and tumour size to be predictors of microvascular invasion in patients with HCC, prior to surgery. Serum level of miR-4463 was reported by Hu et al[60] to be significantly higher in HCC patients, no matter the sex of the patients, the size of the nodule, the stage of the HCC, the pathological type, or the values of the other serum factor tests ( i.e ., ALT, aspartate aminotransferase, total bilirubin, and HBsAg status). In that study the highest level of miRNAs was found in the group of patients with the lowest level of AFP and shorter survival time[60].…”