The potential role of two LEAFY orthologs in the chasmogamous/cleistogamous mixed breeding system of<i>Viola pubescens</i>(Violaceae)<sup>1</sup>

Wang, Yunjing; Ballard, Harvey E.; Sternberger, Anne L.; Wyatt, Sarah E.; Stockinger, Eric J.; Nadella, Vijayanand

doi:10.3159/torrey-d-16-00023r2

Cited by 4 publications

(3 citation statements)

References 52 publications

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“…Overexpression of the LFY gene causes early flowering in many plant species, although the overall plant morphology remains normal [ 7 , 29 , 31 , 32 ]. However, some differences do occur.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Functional Characterization of a LEAFY Gene in Mango (Mangifera indica L.)

Wang

et al. 2022

IJMS

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LEAFY (LFY) plays an important role in the flowering process of plants, controlling flowering time and mediating floral meristem differentiation. Owing to its considerable importance, the mango LFY gene (MiLFY; GenBank accession no. HQ585988) was isolated, and its expression pattern and function were characterized in the present study. The cDNA sequence of MiLFY was 1152 bp, and it encoded a 383 amino acid protein. MiLFY was expressed in all tested tissues and was highly expressed in flowers and buds. Temporal expression analysis showed that MiLFY expression was correlated with floral development stage, and two relative expression peaks were detected in the early stages of floral transition and floral organ differentiation. Moreover, 35S::GFP-MiLFY fusion protein was shown to be localized to the nucleus of cells. Overexpression of MiLFY in Arabidopsis promoted early flowering and the conversion of lateral meristems into terminal flowers. In addition, transgenic plants exhibited obvious morphological changes, such as differences in cauline leaf shape, and the number of lateral branches. When driven by the MiLFY promoter, GFP was highly expressed in leaves, floral organs, stems, and roots, during the flowering period. Exogenous gibberellin (GA3) treatment downregulated MiLFY promoter expression, but paclobutrazol (PPP333) upregulated it. Bimolecular fluorescence complementation (BiFC) assays showed that the MiLFY protein can interact with zinc-finger protein 4 (ZFP4) and SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (MiSOC1D). Taken together, these results indicate that MiLFY plays a pivotal role in controlling mango flowering, and that it is regulated by gibberellin and paclobutrazol.

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“…Overexpression of the LFY gene causes early flowering in many plant species, although the overall plant morphology remains normal [ 7 , 29 , 31 , 32 ]. However, some differences do occur.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Functional Characterization of a LEAFY Gene in Mango (Mangifera indica L.)

Wang

et al. 2022

IJMS

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“…For example, transgenic Arabidopsis lines overexpressing the FcLFY gene flower approximately 6 and 8 days earlier than WT or the corresponding mutant lfy-15 [ 13 ]. Overexpression of VpLFY2 causes precocious flowering in Arabidopsis [ 24 ]. Similar results were also found in Brassica juncea [ 25 ], jatropha [ 15 ], and mango [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

Identification and functional analysis of the LEAFY gene in longan flower induction

Jue,

Li,

Zhang

et al. 2024

BMC Genomics

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Background Flowering at the right time is a very important factor affecting the stable annual yield of longan. However, a lack of knowledge of the regulatory mechanism and key genes of longan flowering restricts healthy development of the longan industry. Therefore, identifying relevant genes and analysing their regulatory mechanism are essential for scientific research and longan industry development. Results DlLFY (Dimocarpus longan LEAFY) contains a 1167 bp open reading frame and encodes 388 amino acids. The amino acid sequence has a typical LFY/FLO family domain. DlLFY was expressed in all tissues tested, except for the leaf, pericarp, and pulp, with the highest expression occurring in flower buds. Expression of DlLFY was significantly upregulated at the early flower induction stage in “SX” (“Shixia”). The results of subcellular localization and transactivation analysis showed that DlLFY is a typical transcription factor acting as a transcriptional activator. Moreover, overexpression of DlLFY in Arabidopsis promoted early flowering and restrained growth, resulting in reduced plant height and rosette leaf number and area in transgenic plants. DNA affinity purification sequencing (DAP-Seq) analysis showed that 13 flower-related genes corresponding to five homologous genes of Arabidopsis may have binding sites and be putative target genes. Among these five flower-related genes, only AtTFL1 (terminal flower 1) was strongly inhibited in transgenic lines. Conclusion Taken together, these results indicate that DlLFY plays a pivotal role in controlling longan flowering, possibly by interacting with TFL1.

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“…The red-light and blue-light receptor genes, PHY and CRY , were differentially expressed; CRY expression was decreased, while the expression of GI was increased, with later increases observed in the expression of CO , which promotes M. sinostellata flowering. LFY , which is expressed in the meristem, is considered to be the switch that determines flower development [ 60 , 61 ]. The expression of LFY was significantly altered during flower bud differentiation in M. sinostellata , indicating that LFY also plays a key role in the development of magnolia flowers.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Analysis of Flower Bud Differentiation in Magnolia sinostellata

Fan¹,

MengQian²,

Dong³

et al. 2018

Genes

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Magnolias are widely cultivated for their beautiful flowers, but despite their popularity, the molecular mechanisms regulating flower bud differentiation have not been elucidated. Here, we used paraffin sections and RNA-seq to study the process of flower bud differentiation in Magnolia sinostellata. Flower bud development occurred between 28 April and 30 May 2017 and was divided into five stages: undifferentiated, early flower bud differentiation, petal primordium differentiation, stamen primordium differentiation, and pistil primordium differentiation. A total of 52,441 expressed genes were identified, of which 11,592 were significantly differentially expressed in the five bud development stages. Of these, 82 genes were involved in the flowering. In addition, MADS-box and AP2 family genes play critical roles in the formation of flower organs and 20 differentially expressed genes associated with flower bud differentiation were identified in M. sinostellata. A qRT-PCR analysis verified that the MADS-box and AP2 family genes were expressed at high levels during flower bud differentiation. Consequently, this study provides a theoretical basis for the genetic regulation of flowering in M. sinostellata, which lays a foundation for further research into flowering genes and may facilitate the development of new cultivars.

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The potential role of two LEAFY orthologs in the chasmogamous/cleistogamous mixed breeding system ofViola pubescens(Violaceae)¹

Cited by 4 publications

References 52 publications

Isolation and Functional Characterization of a LEAFY Gene in Mango (Mangifera indica L.)

Isolation and Functional Characterization of a LEAFY Gene in Mango (Mangifera indica L.)

Identification and functional analysis of the LEAFY gene in longan flower induction

Transcriptomic Analysis of Flower Bud Differentiation in Magnolia sinostellata

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The potential role of two LEAFY orthologs in the chasmogamous/cleistogamous mixed breeding system ofViola pubescens(Violaceae)1

Cited by 4 publications

References 52 publications

Isolation and Functional Characterization of a LEAFY Gene in Mango (Mangifera indica L.)

Isolation and Functional Characterization of a LEAFY Gene in Mango (Mangifera indica L.)

Identification and functional analysis of the LEAFY gene in longan flower induction

Transcriptomic Analysis of Flower Bud Differentiation in Magnolia sinostellata

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The potential role of two LEAFY orthologs in the chasmogamous/cleistogamous mixed breeding system ofViola pubescens(Violaceae)¹