The potential role of ozone in ameliorating the age-related biochemical changes in male rat cerebral cortex

Abstract

Controlled ozone (O(3)) administration is known to promote oxidative preconditioning and, thus, may reverse chronic oxidative stress that accompanies aging. Therefore, the present work was undertaken to study the potential role of O(3) in ameliorating certain age-related biochemical changes represented by impaired activities of inner mitochondrial membrane enzymes, compromised energy production and increased oxidative burden in male rat cerebral cortex. Prophylactic administration of O(3)-O(2) mixture to 3 month-old rats, at an intrarectal dose of 0.6 mg O(3) kg(-1) body weight twice/week for 3 months then once/week until the age of 15 months, normalized reduced glutathione content, adenosine triphosphate/adenosine diphosphate ratio, mitochondrial superoxide dismutase (SOD) and complex IV (cytochrome-c oxidase) activities, improved glutathione redox index (GSHRI), complex I (NADH-ubiquinone oxidoreductase) and mitochondrial nitric oxide synthase (mtNOS) activities, and attenuated the rise in malondialdehyde (MDA) and mitochondrial protein carbonyl levels. On the other hand, therapeutic administration of the same dose of O(3)-O(2) mixture to 14 month-old rats three times/week for 1 month, reduced mitochondrial protein carbonyl level only. Other favorable effects, including normalization of Na,K-adenosine triphosphatase (Na,K-ATPase) activity and reduction in lipofuscin level in the prophylactic group, as well as improvement in mitochondrial SOD and complex I activities with a decrease in total MDA level in the therapeutic group, were comparable to the effects observed in the corresponding O(2)-treated control groups. In conclusion, the present study revealed that prophylactic administration of O(3)-O(2) mixture provided better amelioration of age-related cerebrocortical alterations by combining the advantages of both O(3) and O(2) therapies.

Ozone Autohemotherapy Induces Long-Term Cerebral Metabolic Changes in Multiple Sclerosis Patients

“…The most relevant metabolic effect is the marked increase of the CYT-c an hour after the end of the blood reinjection. This result is in accordance with studies regarding aging, in which it was shown that administration of 0/03 mix ameliorated the age-related cerebrocortical alterations, including the complex IV (CYT-c) activity (28).…”

Increased Growth Factors Play a Role in Wound Healing Promoted by Noninvasive Oxygen-Ozone Therapy in Diabetic Patients with Foot Ulcers

“…Most recently, ozone administered was reported to prevent atherosclerosis development and increase antioxidant systems in New Zealand white rabbits [13]. The antioxidative effects of ozone also ameliorate the age-related biochemical changes in male rat cerebral cortex [14]. In patients with coronary artery disease (CAD), ozone treatment by rectal insufflation significantly improved prothrombin time, reduced biomarkers of protein and lipid oxidation, and increased total antioxidant [15].…”

Ozone: a natural bioactive molecule with antioxidant property as potential new strategy in aging and in neurodegenerative disorders

“…Similarly, prophylactic administration of O 3 in aged-rats normalized reduced GSH content, adenosine triphosphate/adenosine diphosphate ratio, mitochondrial SOD and complex IV (cytochrome-c oxidase) activities. O 3 improved glutathione redox index (GSHRI), complex I (NADH-ubiquinone oxidoreductase) and mitochondrial mtNOS activities, and attenuated the rise MDA and mitochondrial PC levels ( Shehata et al, 2012 ).…”