The potential role of N7-methylguanosine (m7G) in cancer

Luo, Yuejun; Yao, Yuxin; Wu, Peng; Zi, Xiaohui; Sun, Nan; He, Jié

doi:10.1186/s13045-022-01285-5

Cited by 119 publications

(131 citation statements)

References 87 publications

(148 reference statements)

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“…The comparison of the mutations between the subgroups showed a higher TMB in the high-risk group than the low-risk group, as well as some oncogenes, including KRAS ; also, the survival probability in high TMB was better, especially for those with a low-risk level ( Figure 8 ). Reportedly, m7G could reshape the microenvironment, especially the immune cell infiltration ( 15 ). Combined with functional analyses, we further compared the enrichment scores of 16 types of immune cells and the activity of 13 immune-related pathways between the low and high-risk groups employing ssGSEA.…”

Section: Resultsmentioning

confidence: 99%

A novel defined m7G regulator signature to investigate the association between molecular characterization and clinical significance in lung adenocarcinoma

Dong

Yao

et al. 2022

Front. Oncol.

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BackgroundAbout170 chemical modifications to RNAs have been identified, which significantly affect gene expression. Dysregulation of RNA modifications induced by abnormal expression or mutations in RNA modifiers might result in cancer. The most frequent RNA modifications are N6-methyladenosine (m6A), 5-methylcytosine (m5C), and N7-methylguanosine (m7G). Lung cancer is the leading cause of cancer-related deaths globally. The present study aimed to investigate whether the expression of the m7G-related genes is linked to lung cancer cases with lung adenocarcinoma (LUAD), which accounts for about 40% of lung cancer cases.MethodsA total of 12 m7G-related differentially expressed genes (DEGs) were identified in LUAD patients by The Cancer Genome Atlas (TCGA). The least absolute shrinkage and selection operator (LASSO) Cox regression method was used to build a four-gene risk model. Then, LUAD patients in the TCGA cohort were divided into low- and high-risk groups based on their risk scores for subsequent molecular and clinical research.ResultsCompared to the low-risk group, the high-risk group had a decreased overall survival (OS) (P=0.047). The risk score and stage were independent factors for predicting the OS of LUAD (P=0.0004 and P<0.0001, respectively). Gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses based on the two groups showed that the DEGs were metabolically and hormonally related. The high-risk group showed a higher mutation rate and lesser immune cell infiltration, especially in TP53, KRAS, and MET. The expression level of PD-L1 and CTLA4 was high in the high-risk group (P<0.05). The high-risk group is more sensitive to anti-cancer therapy with lower IC50 and higher immunophenoscore (IPS).ConclusionsIn this study, we developed a novel LUAD stratification model based on m7G-related genes that successfully predicts the prognosis of LUAD patients and serves as a guide for clinically personalized treatment.

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Section: Resultsmentioning

confidence: 99%

A novel defined m7G regulator signature to investigate the association between molecular characterization and clinical significance in lung adenocarcinoma

Dong

Yao

et al. 2022

Front. Oncol.

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“…M7G modification is one of the most common forms of base modification in post-transcriptional regulation. It is widely distributed among tRNA, rRNA, and the 5′ cap of eukaryotic mRNA, and plays an important role in 'RNA processing, metabolism, stability, and nucleation, as well as translation (9)(10)(11). Previous studies have found that m7G tRNA modification enhances oncogenic mRNA translation and promotes the progression of intrahepatic cholangiocarcinoma (9).…”

Section: Introductionmentioning

confidence: 99%

“…It is widely distributed among tRNA, rRNA, and the 5′ cap of eukaryotic mRNA, and plays an important role in 'RNA processing, metabolism, stability, and nucleation, as well as translation (9)(10)(11). Previous studies have found that m7G tRNA modification enhances oncogenic mRNA translation and promotes the progression of intrahepatic cholangiocarcinoma (9). However, few studies have verified whether m7G-related genes play a key role in GC.…”

Section: Introductionmentioning

confidence: 99%

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Construction and Validation of a m7G-Related Gene-Based Prognostic Model for Gastric Cancer

Wang

Chen

et al. 2022

Front. Oncol.

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BackgroundGastric cancer (GC) is one of the most common malignant tumors of the digestive system. Chinese cases of GC account for about 40% of the global rate, with approximately 1.66 million people succumbing to the disease each year. Despite the progress made in the treatment of GC, most patients are diagnosed at an advanced stage due to the lack of obvious clinical symptoms in the early stages of GC, and their prognosis is still very poor. The m7G modification is one of the most common forms of base modification in post-transcriptional regulation, and it is widely distributed in the 5′ cap region of tRNA, rRNA, and eukaryotic mRNA.MethodsRNA sequencing data of GC were downloaded from The Cancer Genome Atlas. The differentially expressed m7G-related genes in normal and tumour tissues were determined, and the expression and prognostic value of m7G-related genes were systematically analysed. We then built models using the selected m7G-related genes with the help of machine learning methods.The model was then validated for prognostic value by combining the receiver operating characteristic curve (ROC) and forest plots. The model was then validated on an external dataset. Finally, quantitative real-time PCR (qPCR) was performed to detect gene expression levels in clinical gastric cancer and paraneoplastic tissue.ResultsThe model is able to determine the prognosis of GC samples quantitatively and accurately. The ROC analysis of model has an AUC of 0.761 and 0.714 for the 3-year overall survival (OS) in the training and validation sets, respectively. We determined a correlation between risk scores and immune cell infiltration and concluded that immune cell infiltration affects the prognosis of GC patients. NUDT10, METTL1, NUDT4, GEMIN5, EIF4E1B, and DCPS were identified as prognostic hub genes and potential therapeutic agents were identified based on these genes.ConclusionThe m7G-related gene-based prognostic model showed good prognostic discrimination. Understanding how m7G modification affect the infiltration of the tumor microenvironment (TME) cells will enable us to better understand the TME’s anti-tumor immune response, and hopefully guide more effective immunotherapy methods.

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“…m 7 G is an abundant modification found in the 5′ guanosine cap of mRNAs, as well as internally in mRNAs, tRNAs, rRNAs, and miRNAs [ 102 ]. m 7 G is critical for the complete maturation of the 5′ guanosine cap found in all RNAs transcribed by RNA pol II [ 103 ].…”

Section: The Epitranscriptome In Rnamentioning

confidence: 99%

The Epitranscriptome in miRNAs: Crosstalk, Detection, and Function in Cancer

Valle-Morales

Saviana

et al. 2022

Genes

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The epitranscriptome encompasses all post-transcriptional modifications that occur on RNAs. These modifications can alter the function and regulation of their RNA targets, which, if dysregulated, result in various diseases and cancers. As with other RNAs, miRNAs are highly modified by epitranscriptomic modifications such as m6A methylation, 2′-O-methylation, m5C methylation, m7G methylation, polyuridine, and A-to-I editing. miRNAs are a class of small non-coding RNAs that regulates gene expression at the post-transcriptional level. miRNAs have gathered high clinical interest due to their role in disease, development, and cancer progression. Epitranscriptomic modifications alter the targeting, regulation, and biogenesis of miRNAs, increasing the complexity of miRNA regulation. In addition, emerging studies have revealed crosstalk between these modifications. In this review, we will summarize the epitranscriptomic modifications—focusing on those relevant to miRNAs—examine the recent crosstalk between these modifications, and give a perspective on how this crosstalk expands the complexity of miRNA biology.

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The potential role of N7-methylguanosine (m7G) in cancer

Cited by 119 publications

References 87 publications

A novel defined m7G regulator signature to investigate the association between molecular characterization and clinical significance in lung adenocarcinoma

A novel defined m7G regulator signature to investigate the association between molecular characterization and clinical significance in lung adenocarcinoma

Construction and Validation of a m7G-Related Gene-Based Prognostic Model for Gastric Cancer

The Epitranscriptome in miRNAs: Crosstalk, Detection, and Function in Cancer

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