The Potential Role of miRNAs as Predictive Biomarkers in Neurodevelopmental Disorders

Juvale, Iman Imtiyaz Ahmed; Has, Ahmad Tarmizi Che

doi:10.1007/s12031-021-01825-7

Cited by 9 publications

(4 citation statements)

References 251 publications

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“…Human ADHD is characterized by a dysfunction in activity, attention, and impulse control. Mouse models of ADHD are heterogeneous and diverse, thereby substantiating evidence towards the polygenic nature of ADHD epidemiology [ 62 ].…”

Section: Discussionmentioning

confidence: 99%

The Exon Junction Complex Factor RBM8A in Glial Fibrillary Acid Protein-Expressing Astrocytes Modulates Locomotion Behaviors

Asthana,

Mott,

Tong

et al. 2024

Cells

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The role of RNA Binding Motif Protein 8a (RBM8A), an exon junction complex (EJC) component, in neurodevelopmental disorders has been increasingly studied for its crucial role in regulating multiple levels of gene expression. It regulates mRNA splicing, translation, and mRNA degradation and influences embryonic development. RBM8A protein is expressed in both neurons and astrocytes, but little is known about RBM8A’s specific role in glial fibrillary acid protein (GFAP)-positive astrocytes. To address the role of RBM8A in astrocytes, we generated a conditional heterozygous knockout (KO) mouse line of Rbm8a in astrocytes using a GFAP-cre line. We confirmed a decreased expression of RBM8A in astrocytes of heterozygous conditional KO mice via RT-PCR and Sanger sequencing, as well as qRT-PCR, immunohistochemistry, and Western blot. Interestingly, these mice exhibit significantly increased movement and mobility, alongside sex-specific altered anxiety in the open field test (OFT) and elevated plus maze (OPM) tests. These tests, along with the rotarod test, suggest that these mice have normal motor coordination but hyperactive phenotypes. In addition, the haploinsufficiency of Rbm8a in astrocytes leads to a sex-specific change in astrocyte density in the dentate gyrus. This study further reveals the contribution of Rbm8a deletion to CNS pathology, generating more insights via the glial lens of an Rbm8a model of neurodevelopmental disorder.

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Section: Discussionmentioning

confidence: 99%

The Exon Junction Complex Factor RBM8A in Glial Fibrillary Acid Protein-Expressing Astrocytes Modulates Locomotion Behaviors

Asthana,

Mott,

Tong

et al. 2024

Cells

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“…As zebrafish gains increasing momentum in the field of aging, several different zebrafish models and experiments will be performed in these areas to validate and further expand the animal model with different strains and high-output platforms. Other possible biomarkers for cancer, disorders of neurological development, hepatotoxicity, and other conditions also have been identified in zebrafish, providing novel insights into the identification of molecular pathways and the early detection of these pathologies [44][45][46].…”

Section: Pathogenesis Of Sarcopeniamentioning

confidence: 99%

Zebrafish as an Emerging Model for Sarcopenia: Considerations, Current Insights, and Future Directions

Callegari,

Mirzaei,

Agbaria

et al. 2023

IJMS

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Sarcopenia poses a significant challenge to public health and can severely impact the quality of life of aging populations. Despite extensive efforts to study muscle degeneration using traditional animal models, there is still a lack of effective diagnostic tools, precise biomarkers, and treatments for sarcopenia. Zebrafish models have emerged as powerful tools in biomedical research, providing unique insights into age-related muscle disorders like sarcopenia. The advantages of using zebrafish models include their rapid growth outside of the embryo, optical transparency during early developmental stages, high reproductive potential, ease of husbandry, compact size, and genetic tractability. By deepening our understanding of the molecular processes underlying sarcopenia, we may develop novel diagnostic tools and effective treatments that can improve the lives of aging individuals affected by this condition. This review aims to explore the unique advantages of zebrafish as a model for sarcopenia research, highlight recent breakthroughs, outline potential avenues for future investigations, and emphasize the distinctive contributions that zebrafish models offer. Our research endeavors to contribute significantly to address the urgent need for practical solutions to reduce the impact of sarcopenia on aging populations, ultimately striving to enhance the quality of life for individuals affected by this condition.

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“…MiRNAs have gained significant recognition for their roles in neuropsychiatric disorders, with extensive research in recent decades focusing on their impacts on neurodevelopmental conditions like ADHD, autism, and Alzheimer's disease. These studies primarily explore the influences of miRNAs on cognitive, behavioral, memory, and learning deficits [11][12][13]. Notably, a recent study indicated that the miRNA expression profile, including miR-4516, miR-6090, miR-4763-3p, miR-4281, and miR-4466, has great diagnostic accuracy and specificity in assessing ADHD [14].…”

Section: Introductionmentioning

confidence: 99%

Unraveling the Role of miR-200b-3p in Attention-Deficit/Hyperactivity Disorder (ADHD) and Its Therapeutic Potential in Spontaneously Hypertensive Rats (SHR)

Chang,

Lin,

Tzang

et al. 2024

Biomedicines

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Attention-deficit/hyperactivity disorder (ADHD) is a prevalent neurodevelopmental disorder in children with unknown etiology. Impaired learning ability was commonly reported in ADHD patients and has been associated with dopamine uptake in the striatum of an animal model. Another evidence also indicated that micro-RNA (miR)-200b-3p is associated with learning ability in various animal models. However, the association between miR-200b-3p and ADHD–related symptoms remains unclear. Therefore, the current study investigated the role of miR-200b-3p in ADHD-related symptoms such as inattention and striatal inflammatory cytokines. To verify the influence of miR-200b-3p in ADHD-related symptoms, striatal stereotaxic injection of miR-200b-3p antagomir (AT) was performed on spontaneously hypertensive rats (SHR). The antioxidant activity and expressions of miR-200b-3p, slit guidance ligand 2 (Slit2), and inflammatory cytokines in the striatum of SHR were measured using quantitative real-time polymerase chain reaction (RT-qPCR), immunohistochemistry (IHC), immunoblotting, and enzyme-linked immunosorbent assay (ELISA). The spontaneous alternation of SHR was tested using a three-arm Y-shaped maze. The administration of miR-200b-3p AT or taurine significantly decreased striatal tumor necrosis factor (TNF)-α, interleukin (IL)-1β, and IL-6 in SHR, along with increased super-oxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities and significantly higher spontaneous alternation. In this paper, we show that miR-200b-3p AT and taurine alleviates ADHD-related symptoms in SHR. These findings provide insights into ADHD’s molecular basis and suggest miR-200b-3p as a potential therapeutic target. Concurrently, this study also suggests broad implications for treating neurodevelopmental disorders affecting learning activity such as ADHD.

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The Potential Role of miRNAs as Predictive Biomarkers in Neurodevelopmental Disorders

Cited by 9 publications

References 251 publications

The Exon Junction Complex Factor RBM8A in Glial Fibrillary Acid Protein-Expressing Astrocytes Modulates Locomotion Behaviors

The Exon Junction Complex Factor RBM8A in Glial Fibrillary Acid Protein-Expressing Astrocytes Modulates Locomotion Behaviors

Zebrafish as an Emerging Model for Sarcopenia: Considerations, Current Insights, and Future Directions

Unraveling the Role of miR-200b-3p in Attention-Deficit/Hyperactivity Disorder (ADHD) and Its Therapeutic Potential in Spontaneously Hypertensive Rats (SHR)

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