The potential role of <i>in vivo</i> reflectance confocal microscopy for evaluating oral cavity lesions: a systematic review

Lucchese, Alberta; Gentile, Enrica; Romano, Antonio; Maio, Claudio Di; Laino, Luigi; Serpico, Rosario

doi:10.1111/jop.12454

Cited by 34 publications

(32 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, even though the included case-control studies 14,15,18,21 were classified as high quality assessments, this study design along with the cross sectional design of the other four articles 16,17,19,20 demand careful interpretation of their results. 32 Caution should also be taken in the interpretation of the findings presented herein, as although the study focused on reliable and specific biomarkers to detect Treg and Th17 other biomarkers are known to be involved in the Treg/Th17 imbalance. 14,15 Suggestions for future research…”

Section: Limitationsmentioning

confidence: 99%

Treg and Th17 cells in inflammatory periapical disease: a systematic review

et al. 2017

View full text Add to dashboard Cite

The process involved in periapical lesions, which occur as an outcome of pulpal necrosis, is regulated by the immune system including regulatory T cells (Treg) and T helper 17 cell (Th17) responses. The objective of this study was to conduct a frequency systematic review to determine the presence of Treg/Th17 responses and the influence of these cells in the progression of chronic inflammatory periapical lesions in humans. A systematic computerized search was carried out in Pubmed, Medline, Web of Science and Scopus electronic databases from their date of inception through the first week of May 2017. In addition, the reference lists of the included articles and the grey literature were hand-searched. Articles that evaluated the presence and influence of Treg/Th17 in the progression of human periapical lesions were included. Study selection and the quality assessment of the included articles (using the Newcastle-Ottawa scale) were carried out by two authors. Fifty-seven titles/abstracts were screened and eight studies met the eligibility criteria and were included in this systematic review. The included studies showed large variation in the type of periapical lesion assessed, mean age, age range, type of experiment and findings regarding the participation of Th17 and Treg in the status of inflammatory periapical lesions. The studies showed the involvement of Treg in the modulation of the inflammatory response in radicular cysts and periapical granulomas. This systematic review highlights the relationship between Treg and Th17 acting in a subtle balance inhibiting or promoting the progression of human periapical lesions.

show abstract

Section: Limitationsmentioning

confidence: 99%

Treg and Th17 cells in inflammatory periapical disease: a systematic review

et al. 2017

View full text Add to dashboard Cite

show abstract

“…We present example RCM images and video-mosaics, obtained in healthy volunteer subjects and patients from both clinical and surgical settings (Figs. [3][4][5][6][7][8][9]). The feasibility of video-mosaicking is demonstrated at two key clinically relevant depths.…”

Section: Resultsmentioning

confidence: 99%

“…Figures [3][4][5][6][7] show example cases of RCM imaging and video-mosaicking in normal mucosa on volunteers in the clinical setting. In the epithelium and at the epithelial junction, we observe honeycomb pattern of cells with cobblestone pattern of nuclei.…”

Section: Normal Tissuementioning

confidence: 99%

“…To address this unmet need, a number of optical imaging and spectroscopic techniques are being developed for noninvasive detection of oral premalignancies and malignancies, which may serve as an adjunct for conventional oral examination [4][5][6][7][8][9][10][11]. The pace of development has been remarkably rapid; we present only a quick summary while referring the reader to the vast literature for the current state of translation and clinical testing.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Feasibility of a Video‐Mosaicking Approach to Extend the Field‐of‐View For Reflectance Confocal Microscopy in the Oral Cavity In Vivo

et al. 2019

View full text Add to dashboard Cite

Background Reflectance confocal microscopy (RCM) is a developing approach for noninvasive detection of oral lesions with label‐free contrast and cellular‐level resolution. For access into the oral cavity, confocal microscopes are being configured with small‐diameter telescopic probes and small objective lenses. However, a small probe and objective lens allows for a rather small field‐of‐view relative to the large areas of tissue that must be examined for diagnosis. To extend the field‐of‐view for intraoral RCM imaging, we are investigating a video‐mosaicking approach. Methods A relay telescope and objective lens were adapted to an existing confocal microscope for access into the oral cavity. Imaging was performed using metal three‐dimensional‐printed objective lens front‐end caps with coverslip windows to contact and stabilize the tissue and set depth. Four healthy volunteers (normal oral mucosa), one patient (with an amalgam tattoo) in a clinical setting, and 20 anesthetized patients (with oral squamous cell carcinoma [OSCC]) in a surgical setting were imaged. Instead of the usual still RCM images, videos were recorded and then processed into video‐mosaics. Thirty video‐mosaics were read and qualitatively assessed by an expert reader of RCM images of the oral mucosa. Results Whereas the objective lens’ native field‐of‐view is 0.75 mm × 0.75 mm, the video‐mosaics display larger areas, ranging from 2 mm × 2 mm to 4 mm × 2 mm, with resolution, morphologic detail, and image quality that is preserved relative to that observed in the original videos (individual images). Video‐mosaics in healthy volunteers’ and the patients’ images showed cellular morphologic patterns in the lower epithelium and at the epithelial junction, and connective tissue along with capillary loops and blood flow in the deeper lamina propria. In OSCC, tumor nests could be observed along with normal looking mucosa in margin areas. Conclusions Video‐mosaicking is a reasonably quick and efficient approach for extending the field‐of‐view of RCM imaging, which can, to some extent, overcome the inherent limitation of an intraoral probe's small field‐of‐view. Reading video‐mosaics can mimic the procedure for examining pathology: initial visualization of the spatial cellular and morphologic patterns of the tumor and the spread of tumor margins over larger areas of the lesion, followed by digitally zooming (magnifying) for closer inspection of suspicious areas. However, faster processing of videos into video‐mosaics will be necessary, to allow examination of video‐mosaics in real‐time at the bedside. Lasers Surg. Med. 51:439–451, 2019. © 2019 Wiley Periodicals, Inc.

show abstract

“…But due to the large rigid probes, some areas are difficult to visualize and access to all parts of the oral cavity are limited. Moreover, motion artefacts are seen due to the poor stability of soft tissue intra orally [51][52][53] .…”

Section: Diagnosis Of Potentially Malignant Oral Lesionsmentioning

confidence: 99%

Potansiyel Malign Oral Lezyonların Belirlenmesi

Kamath¹,

Chatra²

2017

Arşiv Kaynak Tarama Dergisi

View full text Add to dashboard Cite

Oral cancer has reached its magnificent height with newer victims being added everyday into its registry. Conditions that predispose to cancer must be identified at its earliest. Diagnostic aids that are currently employed, identify lesions with morphological alteration at a stage where frank malignancy has already set in. There seems to be a great urgency to identify novel biomarkers to diagnose the malignant potential in these habit-associated lesions and prevent the ever-increasing number of oral cancer cases. The following review addresses the current diagnostic aids used, with its shortcomings and future trends in proteomics, which should become a reality.

show abstract

The potential role of in vivo reflectance confocal microscopy for evaluating oral cavity lesions: a systematic review

Cited by 34 publications

References 23 publications

Treg and Th17 cells in inflammatory periapical disease: a systematic review

Treg and Th17 cells in inflammatory periapical disease: a systematic review

Feasibility of a Video‐Mosaicking Approach to Extend the Field‐of‐View For Reflectance Confocal Microscopy in the Oral Cavity In Vivo

Potansiyel Malign Oral Lezyonların Belirlenmesi

Contact Info

Product

Resources

About