The potential role of granulosa cells in the maturation rate of immature human oocytes and embryo development: A co-culture study

Jahromi, Bahia Namavar; Mosallanezhad, Zahra; Matloob, Najmeh; Davari, Maryam; Ghobadifar, Mohamed Amin

doi:10.5653/cerm.2015.42.3.111

Cited by 39 publications

(30 citation statements)

References 41 publications

(51 reference statements)

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“…Culture of COCs in medium containing granulosa secreted factors has been suggested to increase the proximity of in vitro culture to in vivo condition and improve the efficiency of in vitro oocyte maturation, especially in COCs derived from small follicles (2 to � 4mm) with lower developmental competence compared to follicles of � 4 to 6mm or of greater size [29,30]. Our goal is to select ovine as a model for human IVM in future studies.…”

Section: Introductionmentioning

confidence: 99%

Granulosa secreted factors improve the developmental competence of cumulus oocyte complexes from small antral follicles in sheep

et al. 2020

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Oocyte in vitro maturation can be improved by mimicking the intra-follicular environment. Oocyte, cumulus cells, granulosa cells, and circulating factors act as meiotic regulators in follicles and maintain oocyte in the meiotic phase until oocyte becomes competent and ready to be ovulated. In a randomized experimental design, an ovine model was used to optimize the standard in vitro maturation media by Granulosa secreted factors. At first, the development capacity of oocyte derived from medium (>4 to 6 mm) and small (2 to �4 mm) size follicles was determined. Differential gene expression of granulosa secreted factors and their receptors were compared between the cumulus cells of the two groups. Then, the best time and concentration for arresting oocytes at the germinal vesicle stage by natriuretic peptide type C (CNP) were determined by nuclear staining in both groups. Oocyte quality was further confirmed by calcein uptake and gene expression. The developmental competence of cumulus oocyte complexes derived from small size follicles that were cultured in the presence of CNP in combination with amphiregulin (AREG) and prostaglandin E2 (PGE2) for 24 h was determined. Finally, embryo quality was specified by assessing expressions of NANOG, SOX2, CDX2, OCT4, and TET1. The cumulus oocyte complexes derived from small size follicles had a lower capacity to form blastocyst in comparison with cumulus oocyte complexes derived from medium size follicles. Prostaglandin E receptor 2 and prostaglandin-endoperoxide synthase 2 had significantly lower expression in cumulus cells derived from small size follicles in comparison with cumulus cells derived from medium size follicles. Natriuretic peptide type C increased the percentage of cumulus oocyte complexes arresting at the germinal vesicle stage in both oocytes derived from medium and small follicles. Gap junction communication was also improved in the presence of natriuretic peptide type C. In oocytes derived from small size follicles; best blastocyst rates were achieved by sequential exposure of cumulus oocyte complexes in [TCM+CNP (6 h), then cultured in TCM+AREG+PGE2 (18h)] and [TCM+CNP (6 h), then cultured in conventional IVM supple-ments+AREG+PGE2 (18h)]. Increased SOX2 expression was observed in [TCM+CNP (6 h), then cultured in TCM+AREG+PGE2 (18h)], while decreased OCT4 expression was

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Section: Introductionmentioning

confidence: 99%

Granulosa secreted factors improve the developmental competence of cumulus oocyte complexes from small antral follicles in sheep

et al. 2020

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“…In various animal models, the coculture with somatic cells (granulosa, oviductal or embryonic fibroblasts) as companion cells or monolayer, or the culture in isolated oviducts, gave promising results in terms of nuclear and cytoplasmic oocyte maturation (Chigioni, Perego, & Luvoni, ; Lee, Quaas, Wright, Toth, & Teixeira, ; Luvoni, Chigioni, Allievi, & Macis, ). The cleavage, as well as the advanced embryo development, were also enhanced when COCs were matured with companion cells, rather than alone (Hatoya et al., ; Jahromi, Mosallanezhad, Matloob, Davari, & Ghobadifar, ).…”

Section: Introductionmentioning

confidence: 99%

The nuclear and developmental competence of cumulus–oocyte complexes is enhanced by three‐dimensional coculture with conspecific denuded oocytes during in vitro maturation in the domestic cat model

Morselli

Luvoni

Comizzoli

2016

Reprod Domestic Animals

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Contents The objective of the study was to assess the efficacy of coculture with conspecific cumulus‐denuded oocytes (CDOs) during in vitro maturation in a three‐dimensional system of barium alginate microcapsules on the in vitro embryo development of domestic cat cumulus–oocyte complexes (COCs). In Experiment I, COCs were cocultured with conspecific CDOs or cultured separately in a 3D system for 24 hr of in vitro maturation, before assessing the meiotic progression. In Experiment II, the in vitro fertilization of COCs and CDOs was carried out with chilled epididymal spermatozoa and the presumptive zygotes were cultured in vitro separately for 7 days in 3D microcapsules before assesment of embryonic development. The results showed that the viability was maintained and that meiosis was resumed in the 3D culture system. The presence of CDOs during in vitro maturation improved the meiotic competence of the COCs, since the proportions of telophase I/metaphase II were higher than that in the groups cultured separately. The enrichment of the maturation system by companion oocytes also enhanced the ability of COCs to develop into embryos, and increased the percentages of morula and blastoycst stages. The COCs cocultured with CDOs developed at higher rates than the COCs cultured separately and the CDOs themselves. The beneficial effects of coculture with conspecific CDOs were presumably due to the paracrine action of some secreted factors that enhanced many molecular patterns related to the complex of cumulus oophorous cells. Further investigations to understand how the 3D microenvironment can influence the features of oocytes and embryos are required.

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“…Regulatory molecules, glucose metabolites, nucleotides, and amino acids are known to be transferred through the oocyte-GC gap junctions of growing oocytes [31]. GCs were demonstrated to have a considerable improvement in the maturation rate and the likelihood of embryo development [21]. Our data performed the potential mechanism in GCs to provide some evidences linking obesity to poor reproductive outcomes pregnancy outcome.…”

Section: Discussionmentioning

confidence: 68%

“…Moreover, it is known that GCs can protect the oocyte against oxidative stress [20]. In vitro study demonstrated that culturing immature human oocytes with GCs improves the maturation rate and the likelihood of embryo development [21].…”

Section: Introductionmentioning

confidence: 99%

High Leptin Level Attenuates Embryo Development in Overweight/Obese Infertile Women by Inhibiting Proliferation and Promotes Apoptosis in Granule Cell

Lin

Wang

et al. 2017

Horm Metab Res

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Obesity appears to be associated with female reproductive dysfunction and infertility. Women with obesity undergoing in vitro fertilization (IVF) had poor oocyte quality, decreased embryo development, and poor pregnancy outcome. However, the mechanism linking obesity to poor reproductive outcomes is still unclear. Obesity is frequently accompanied with elevated leptin levels. Here we aimed to evaluate the effect of high leptin level in follicular fluid (FF) on the proliferation and apoptosis in granule cells and correlate these findings with poor reproductive outcomes in infertile women with overweight or obesity who underwent IVF treatment. We investigated clinical and ongoing pregnancy rates in 189 infertile women who underwent IVF. Leptin levels were quantified in peripheral blood and FF as well. In vitro cell model was used to explore the potential effect of high leptin on the proliferation and apoptosis in granulosa cells. Results showed reduced clinical and ongoing pregnancy rates in overweight/obesity women who underwent IVF compared to control with normal BMI. On the other hand, leptin levels presented significant increase in peripheral blood and FF in overweight/obese women. Leptin level in FF was negatively correlated to good quality embryo rate. Importantly, in vitro study showed that leptin inhibited cells proliferation and promoted apoptosis by upregulation of caspase-3 and downregulation of Bcl-2 in granulosa cells in a dose dependent manner. These observations suggest that leptin may acts as a local mediator to attenuate embryo development and reduce fertility in obese patients.

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The potential role of granulosa cells in the maturation rate of immature human oocytes and embryo development: A co-culture study

Cited by 39 publications

References 41 publications

Granulosa secreted factors improve the developmental competence of cumulus oocyte complexes from small antral follicles in sheep

Granulosa secreted factors improve the developmental competence of cumulus oocyte complexes from small antral follicles in sheep

The nuclear and developmental competence of cumulus–oocyte complexes is enhanced by three‐dimensional coculture with conspecific denuded oocytes during in vitro maturation in the domestic cat model

High Leptin Level Attenuates Embryo Development in Overweight/Obese Infertile Women by Inhibiting Proliferation and Promotes Apoptosis in Granule Cell

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