The potential protective effect of Ferulic acid against gamma irradiation induced ovarian failure in rats

Abstract

T hIs sTuDy aims to evaluate the radioprotective effect of Ferulic acid (FA) against ovarian damage in premature female rats. Four groups of female rats were used (ten rats in each group). Group (1): Control, Group (2): Irradiation (IRR) (rats were exposed to 4Gy of whole body gamma irradiation as a single dose), Group (3): FA (rats received 50mg/kg b.wt of Ferulic acid orally for two weeks) and Group (4): FA+IRR (rats received Ferulic acid as group 3 then exposed to 4Gy as group2). The rats were sacrificed after 24h of irradiation. The level of FSH, LH, and E 2 were estimated in the serum. The level of H 2 O 2 and GPx activity and the gene expression of cytochrome C, P53 and caspase3 were evaluated in ovarian tissue. Histopathological examination of the ovary and uterus tissues was performed. The results revealed that gamma irradiation caused an elevation in FSH, LH, H 2 O 2 , cytochrome C, P53 and caspase3 and a decline in E 2 and GPx. The administration of FA prior gamma irradiation led to an improvement in female hormones, H 2 O 2 , GPx and apoptotic markers and matched the histopathological examination results. It could be concluded that Ferulic acid may exert a radioprotective effect against irradiation induced apoptosis in the ovary of rats and alteration in the level of serum female hormones.

The effect of temperature and storage time on DNA integrity after freeze-drying sperm from individuals with normozoospermia

“…Furthermore, the freeze-dried groups had significantly lower sperm vitality than the fresh and freeze groups. Damage caused by freeze-drying to motility and vitality has been reported in previous studies [ 8 , 13 ]. The fact that sperm become immotile after the lyophilization process suggests that they had lost their motility (and vitality) due to stresses associated with the drying or hydration processes.…”

“…One month after freezing, the sample was thawed. After thawing, to remove the cryoprotectant, the suspension was washed in T6 medium [ 13 ].…”

“…Then the cryovial was transferred to a programmable freeze-dry machine (Pishtaz Equipment Company) near the condenser and subjected to vacuum treatment overnight at 220×10 −3 mbar pressure. Immediately before evaluation, the vials of freeze-dried sperm were unsealed, and the sperm were hydrated with 500 μL sterile distilled water [ 13 ].…”

“…At least 200 sperm were evaluated via light microscopy at ×1,000 magnification. Next, the percentages of normal and abnormal sperm were reported based on Kruger's classification [ 13 ].…”