The potential of the wild dog rose (<i>Rosa canina</i>) to mitigate <i>in vitro</i> rumen methane production

Abstract

This study examined the potential of liquid wild dog rose (Rosa canina) seeds oil and solid seeds residue obtained after CO 2 extraction in supercritical conditions to mitigate rumen methane production in vitro. Two experiments were carried out. The substrate comprised of a mixture of meadow hay and barley meal (60:40) for the control diets (CON1 in experiment with oil and CON2 in experiment with residue). The control diets were supplemented up to 5% in dry matter of rose seeds oil (RO) and 5% of rose seeds residue (RR). The following parameters were measured: pH, ammonia, volatile fatty acids, ciliate protozoa and bacteria count, methane concentration and methanogens population. In the RO treatment a decrease in methane production and an increase in the Archea population were observed. In the RR treatment no change in methane production was reported, whereas some variations in protozoal populations were detected in relation to CON2. The potential to mitigate methane production was reported only in wild dog rose seeds oil treatment. Besides, no negative effect of wild dog rose seeds residue on rumen processes was stated, what may predispose this protein and fibre containing by-product to be utilized as ruminants dietary ingredient. 286 ROSA CANINA MITIGATE METHANE PRODUCTION IN RUMEN

Comparison of nutrient composition and anti-methanogenic properties of different <i>Rosaceae</i> species

“…In the present experiment, anti-methanogenic activity was found in the plant parts containing from 1.8% to 13.4% of CT, but this effect was not observed in the plant parts containing less than 1.8% of CT. In line with our study, Szumacher-Strabel et al (2011) determined in a ruminal batch culture system study that the seed oil from R. canina reduced methane production.…”

“…Tree forage species are also rich in secondary metabolites such as tannins, and are called tanninferous plants. Recent studies have revealed the inhibitory effect of tannins and tanninferous plants on methane emission by ruminants (Dong et al, 2010;Szumacher-Strabel et al, 2011). The phytochemicals, such as tannins and flavonoids, occurring in the structures of Rosa canina (in fruits, seeds, leaves) may reduce ruminal methane production (Woodward et al, 2002; ABSTRACT.…”

Plant components with specific activities against rumen methanogens

“…Research on the effects of tannins on the populations of methanogens has been carried out both under in vitro and in vivo conditions, however, results were not always unequivocal (Tavendale et al, 2005;Bhatta et al, 2009;Szumacher-Strabel et al, 2011;Cieslak et al, 2012). The range of mitigation of methane production by using tannins is quite broad, from 2% to 58% in comparison with analyzed control groups (Patra and Saxena, 2010a;Bodas et al, 2012), and factors responsible for the mitigation are various, for example, tannin type, plant source.…”

Development of nucleic acid based techniques and possibilities of their application to rumen microbial ecology research

“…This technique is very useful, especially in the estimation of the methanogens population in the rumen (Szumacher-Strabel et al, 2011). The Archaea, which live in the digestion system of ruminants, are very difficult to cultivate in vitro (Garcia et al, 2000).…”