The potential of silk sericin protein as a serum substitute or an additive in cell culture and cryopreservation

Abstract

Cell culture and cryopreservation are necessary for clinical therapy and cells storage. The addition of 10% (v/v) foetal bovine serum (FBS) to basal culture media has been common practice and is one of the most widely used methods. FBS media added with 10% DMSO (dimethyl sulfoxide) have also been used for cryopreservation cells. Ideally, FBS should be avoided because of high cost and bio-safety. Silk sericin has been used as a serum substitute and an additive due to its good hydrophilicity and biological safety. This article summarizes a few details about the processing of sericin and its application as a serum substitute or an additive for cell culture and cryopreservation media. Sericin can be a potential novel serum substitute or an additive for cell culture and cryopreservation media.

Stable and Biocompatible Carbon Nanotube Ink Mediated by Silk Protein for Printed Electronics

“…The influence of sericin and CNTs on the growth of cells are consistent with previous reports. [25,39] Experiments with A549 cells and HUVEC cells also gave similar results ( Figures S9 and S10, Supporting Information). The density of live U87 cells in SSCNT group was comparable with that in the blank group after 3 days of incubation, suggesting the long-term cytocompatibility of sericin-CNT hybrid (Figure 4d; S11, Supporting Information).…”

“…Sericin is one of the two major proteins present in silk cocoons, where it serves as an adhesive "glue" to bond the fibroin-based fibers together. [36,37] Sericin is a promising bio-material with excellent properties, such as good biocompatibility, [38,39] adjustable gelation properties [40] and enhanced cell attachment effects. [41] Besides, fibroin, which is the other main protein in natural silk fibers, is also a well-known biomaterial for its good biocompatibility.…”

Sensitive protein detection and visualization using protein‐binding peptides

“…Silk sericin (SS), as an important silk protein, has been found to be useful in many biomedical fields (Bari, Perteghella, Farago, & Torre, 2018;Bari, Perteghella, Marrubini, et al, 2018;Chen et al, 2018;Gilotra, Chouhan, Bhardwaj, Nandi, & Mandal, 2018), such as wound healing (Bari et al, 2017;Napavichayanun, Yamdech, & Aramwit, 2018;Yang et al, 2017), regenerative medicine (Bari, Perteghella, Farago, et al, 2018;Bari, Perteghella, Marrubini, et al, 2018;Rao et al, 2017;Xie et al, 2015), cell culture matrix (Cao & Zhang, 2017;Terada, Sasaki, Yanagihara, & Yamada, 2005;Verdanova, Pytlik, & Kalbacova, 2014), and drug delivery carriers (Lamboni, Gauthier, Yang, & Wang, 2015;Srisuwan, Baimark, & Srihanam, 2017). For example, Napavichayanun et al (2018) added SS, polyhexamethylene biguanide, and glycerin to bacterial cellulose to improve its performance in wound healing because SS could increase the formation of collagen in wound sites.…”

Advanced Biotechnology for Cell Cryopreservation

“…1 a Schematic diagram for two types of cryoinjuries occurring during cryopreservation of cells; b schematic diagram for various biotechnology techniques used in cryopreservation and their method of protection extracellular cryopreservation. However, FBS is derived from animals and has risk of inducing viral infections and immunogenicity inpatients [20,21].…”