The Potential of Nanoemulsions in Biomedicine

Mazza, Mariarosa; Alonso-Sande, M.; Jones, Marie‐Christine; Fuente, María de la

doi:10.1007/978-1-4614-9164-4_6

Cited by 6 publications

(5 citation statements)

References 200 publications

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“…Although a classical emulsion-type structure had been initially envisaged for these formulations, the formation of a nanosystem containing at least one water pocket was determined by CG-MD simulations and NMR (for the ratio 1:0.1). It is known that, due to its amphipathic structure, vitamin E and analogues can form vesicles at appropriate concentrations; however, such vesicles become easily unstable in the presence of divalent cations, acidic pH, and serum and also over time, they tend to aggregate due to the absence of surfactants that stabilize the droplets. , Considering our concentration range and other formulation conditions, vitamin E, after injection in water, self-arranged into nanovesicles with an average size of over 200 nm and a marked negative charge, which were not stable for more than 4 h (Figure S15). Nevertheless, this destabilization effect is not measurable in the CG-MD simulation time scales (500 ns).…”

Section: Discussionmentioning

confidence: 91%

In Vitro–In Silico Modeling Approach to Rationally Designed Simple and Versatile Drug Delivery Systems

et al. 2020

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Rational design and development of a nanosystem usually relies on empirical approaches as well as a fair degree of serendipity. Understanding how nanosystems behave at the molecular level is of great importance for potential biomedical applications. In this work, we describe a nanosystem composed of two natural compounds, vitamin E and sphingomyelin, prepared by spontaneous emulsification (vitamin E−sphingomyelin nanosystems (VSNs)). Extensive characterization revealed suitable physicochemical properties, very high biocompatibility in vitro and in vivo, and colloidal stability during storage and in biological media, all relevant properties for clinical translation. We have additionally pursued a computational approach to gain an improved understanding of the assembling, structure, dynamics, and drug-loading capacity of VSNs, using both small molecules and biomolecules (resveratrol, curcumin, gemcitabine, and two peptides). The spontaneous formation of compartmentalized VSNs starting from completely disassembled molecules, observed here for the first time, was accurately assessed from the computational molecular dynamics trajectories. We describe here a synergistic in silico/in vitro approach showing the predictive power of computational simulations for VSNs' structural characterization and description of internal interaction mechanisms responsible for the association of bioactive molecules, representing a paradigm shift in the rational design of nanotechnologies as drug delivery systems for advanced personalized medicine.

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Section: Discussionmentioning

confidence: 91%

In Vitro–In Silico Modeling Approach to Rationally Designed Simple and Versatile Drug Delivery Systems

et al. 2020

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“…Furthermore, emulsions can be classified depending on their droplet size as coarse emulsions or nanoemulsions, and there has in fact been some debate on nomenclature when considering nanoemulsions or microemulsions [118]. In this term, nanoemulsions are exclusively formed by spherical nanosized oil droplets; however, they are not thermodynamically stable, as microemulsions are [119]. Although microemulsions can exist in the nanosized ranges [119], we will refer to nanoemulsions here when considering only kinetically nanosized emulsions.…”

Section: Cationic Nanoemulsionsmentioning

confidence: 99%

“…In this term, nanoemulsions are exclusively formed by spherical nanosized oil droplets; however, they are not thermodynamically stable, as microemulsions are [119]. Although microemulsions can exist in the nanosized ranges [119], we will refer to nanoemulsions here when considering only kinetically nanosized emulsions.…”

Section: Cationic Nanoemulsionsmentioning

confidence: 99%

The Potential of Nanomedicine to Unlock the Limitless Applications of mRNA

Taina-González

Fuente

2022

Pharmaceutics

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The year 2020 was a turning point in the way society perceives science. Messenger RNA (mRNA) technology finally showed and shared its potential, starting a new era in medicine. However, there is no doubt that commercialization of these vaccines would not have been possible without nanotechnology, which has finally answered the long-term question of how to deliver mRNA in vivo. The aim of this review is to showcase the importance of this scientific milestone for the development of additional mRNA therapeutics. Firstly, we provide a full description of the marketed vaccine formulations and disclose LNPs’ pharmaceutical properties, including composition, structure, and manufacturing considerations Additionally, we review different types of lipid-based delivery technologies currently in preclinical and clinical development, namely lipoplexes and cationic nanoemulsions. Finally, we highlight the most promising clinical applications of mRNA in different fields such as vaccinology, immuno-oncology, gene therapy for rare genetic diseases and gene editing using CRISPR Cas9.

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“…Typically, cationic nanosystems aimed at gene therapy applications incorporate cationic lipids such as 1,2-Dioleoyloxy-3-Trimethylammoniumpropanchloride (DOTAP), N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA 3-b[N-(N0,N0-dimethylaminoethane)-carbamoyl] cholesterol (DC-Chol), stearylamine (ST) or cetyltrimethylammonium bromide (CTAB) to favor the establishment of electrostatic interactions with anionic nucleic acids [ 26 ]. Emulsions are promising delivery systems that have a broad number of applications in biomedicine [ 27 ]. In a previous study, we observed that DOTAP showed a superior miRNA-complexing capacity than ST, forming stable lipoplexes.…”

Section: Introductionmentioning

confidence: 99%

“…or cetyltrimethylammonium bromide (CTAB) to favor the establishment of electrostatic interactions with anionic nucleic acids [26]. Emulsions are promising delivery systems that have a broad number of applications in biomedicine [27]. In a previous study, we observed that DOTAP showed a superior miRNA-complexing capacity than ST, forming stable lipoplexes.…”

Section: Introductionmentioning

confidence: 99%

Modulation of Colorectal Tumor Behavior via lncRNA TP53TG1-Lipidic Nanosystem

et al. 2021

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Long non-coding RNAs (lncRNAs) are an emerging group of RNAs with a crucial role in cancer pathogenesis. In gastrointestinal cancers, TP53 target 1 (TP53TG1) is an epigenetically regulated lncRNA that represents a promising therapeutic target due to its tumor suppressor properties regulating the p53-mediated DNA damage and the intracellular localization of the oncogenic YBX1 protein. However, to translate this finding into the clinic as a gene therapy, it is important to develop effective carriers able to deliver exogenous lncRNAs to the targeted cancer cells. Here, we propose the use of biocompatible sphingomyelin nanosystems comprising DOTAP (DSNs) to carry and deliver a plasmid vector encoding for TP53TG1 (pc(TP53TG1)-DSNs) to a colorectal cancer cell line (HCT-116). DSNs presented a high association capacity and convenient physicochemical properties. In addition, pc(TP53TG1)-DSNs showed anti-tumor activities in vitro, specifically a decrease in the proliferation rate, a diminished colony-forming capacity, and hampered migration and invasiveness of the treated cancer cells. Consequently, the proposed strategy displays a high potential as a therapeutic approach for colorectal cancer.

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The Potential of Nanoemulsions in Biomedicine

Cited by 6 publications

References 200 publications

In Vitro–In Silico Modeling Approach to Rationally Designed Simple and Versatile Drug Delivery Systems

In Vitro–In Silico Modeling Approach to Rationally Designed Simple and Versatile Drug Delivery Systems

The Potential of Nanomedicine to Unlock the Limitless Applications of mRNA

Modulation of Colorectal Tumor Behavior via lncRNA TP53TG1-Lipidic Nanosystem

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