The potential of multielement stable isotope analysis for regional origin assignment of butter

et al. 2008

Pesq. agropec. bras.

Resumo -O objetivo deste trabalho foi rastrear a inclussão de farinhas de origem animal em rações para frango de corte com ou sem levedura de cana-de-açúcar e farelo de trigo, por meio da análise do músculo peitoral das aves pelas técnicas dos isótopos estáveis de carbono e nitrogênio. Foram utilizados 210 pintos machos (Cobb), com um dia de idade, distribuídos aleatoriamente em sete tratamentos de 30 aves cada, tendo sido um tratamento controle (dieta vegetal) e seis com inclusão de farinha de carne e ossos bovina ou farinha de vísceras de aves na dieta, com ou sem levedura de cana-de-açúcar e farelo de trigo. Aos 42 dias de idade, foram abatidas quatro aves, por tratamento, escolhidas ao acaso, cujo músculo peitoral foi retirado para análise da razão isotópica. Os resultados obtidos foram submetidos à análise multivariada. Os tratamentos experimentais diferiram do tratamento controle, e foi identificada a inclusão de farinha de origem animal, pelas técnicas dos isótopos estáveis, mesmo com inclusão de levedura ou farelo de trigo na dieta.Termos para indexação: Gallus gallus, carbono-13, certificação, farinha de carne e ossos, farinha de vísceras, nitrogênio-15. Traceability of animal byproducts in diets containing yeast and wheat meal for broilersAbstract -The aim of this paper was to trace the presence of meals from animal origin, in diets for broilers with or without yeast and wheat meal, through the analysis of breast muscle, by using carbon and nitrogen stable isotope techniques. Two hundred ten male chicks (Cobb) one-day old were randomly distributed to seven treatments (30 birds each), with a control diet (exclusively vegetal), and six diets containing meat and bone meal or poultry offal meal, with or without yeast and wheat meal in their compositions. At 42 days of age, four broilers randomly chosen, by treatment were slaughtered, and their breast muscles were collected for isotopic ratio analysis. The isotopic results were analyzed by multivariate statistical analysis of variance. The treatments differed from the control and the inclusion of meals of animal origin was identified by stable isotope techniques, even when there was yeast or wheat meal included in the diet.

Section: Introductionunclassified

Rastreabilidade de subprodutos de origem animal em dietas com levedura e trigo para frangos

Gottmann

Pezzato

et al. 2008

Pesq. agropec. bras.

“…However, none of these methods is used to test the final product, i.e., meat, eggs, milk, etc., leaving room for fraud. Mass spectrometry, on the other hand, has been successfully used to test the authenticity and the quality of several products, such as orange juice (Bricout & Koziet, 1987;Koziet et al, 1993), wine (Martin et al, 1988), honey (Brookes et al, 1991;White et al, 1998) and dairy products (Rossmann et al, 2000;Manca et al, 2001). Moreover, that technique has been used to characterize and differentiate the dietary regime of Iberian pigs, allowing their classification according to diet type during finishing (González-Martin et al, 1999) and for the certification of origin and quality of animal products (Hargin, 1996;Monin, 1998;González-Martin et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

The traceability of animal meals in layer diets as detected by stable carbon and nitrogen isotope analyses of eggs

Denadai¹,

Rev. Bras. Cienc. Avic.

Sartori

et al. 2008

The aim of this study was to trace the inclusion of animal meals in layer diets by analyzing eggs and their fractions (yolk and albumen) using the technique of carbon and nitrogen isotopes. Two-hundred and eighty-eight (288) 73-week-old Shaver White layers, never fed animal ingredients, were randomly distributed in six treatments with six replicates each. The treatments were: control -corn and soybean meal based diet and five other experimental diets including bovine meat and bone meal (MBM); poultry offal meal (POM); feather meal (FM); feather meal and poultry offal meal (OFM), and poultry offal meal, feather meal, and meat and bone meal (MBOFM). The isotopic results were submitted to multivariate analysis of variance. Ellipses were determined through an error matrix (95% confidence) to identify differences between treatments and the control group. In the albumen and yolk of all experimental treatments were significantly different from the control diet (p < 0.05). In summary, the stable isotope technique is able to trace the animal meals included in layer feeds in the final product under these experimental conditions.

“…The isotopic ratio of the chemical element carbon has been successfully used to test the authenticity, quality, and geographical origin of several products, such as fruit juice (Bricout & Koziet, 1987;Koziet et al, 1993), wine (Martin et al, 1988), honey (Brookes et al, 1991;Reniero et al, 1997;White et al, 1998), dairy products Rossmann et al, 2000;Manca et al, 2001), and vegetable oils (Kelly et al, 1997), as well as to characterize and to differentiate Iberian pork products, allowing to classify animals according to the type of feeding offered during the finishing period (Gonzáles- Martin et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Traceability of animal byproducts in quail (Coturnix coturnix japonica) tissues using carbon (13C/12C) and nitrogen (15N/14N) stable isotopes

Móri

Garcia

Rev. Bras. Cienc. Avic.

et al. 2007

Consistent information on meat products consumed by the public is essential. The technique of stable isotopes is a powerful tool to recover consumers' confidence, as it allows the detection of animal byproduct residues in poultry meat, particularly in quail meat. This study aimed at checking the presence of poultry byproduct mixtures in quail diets by applying the technique of carbon ( 13 C/ 12 C) and nitrogen ( 15 N/ 14 N) stable isotopes in quail breast muscle, keel, and tibia. Sixty four one-day-old male quails were obtained from a commercial farm. Birds were housed in an experimental house from one to 42 days of age, and were randomly distributed into 8 experimental treatments, and fed diets containing poultry offal meal (POM), bovine meat and bone meal (MBM) or poultry feather meal (PFM), or their mixtures. Four birds per treatment were slaughtered at 42 days of age, and breast (Pectoralis major), keel, and tibia were collected for analyses. The inclusion of animal byproducts in quail diets was detected by 13 C e 15 N analyses in the tissues of the birds; however, it was not possible to specify which byproducts were used. It was concluded that quail meat can be certified by the technique of stable isotopes.