The Potential of Molecular Indicators of Plant Virus Infection: Are Plants Able to Tell Us They Are Infected?

Valmonte-Cortes, Gardette R.; Lilly, Sonia; Pearson, M. N.; Higgins, Colleen M.; MacDiarmid, Robin M.

doi:10.3390/plants11020188

Cited by 3 publications

(3 citation statements)

References 73 publications

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“…It would be interesting to explore whether the reduction of CPK3 diffusion observed upon PlAMV infection is specific to this virus or if it can be extended to other viral species, genera and even pathogens. Indeed, it was recently shown that CPK3 transcription enhanced upon infection by viruses from different genera 54 . Moreover, CPK3 regulates herbivore responses by phosphorylating transcription factors 20 , is activated by flg22 in protoplasts 19 and is proposed to be the target of a bacterial effector to disrupt immune response 21 .…”

Section: Discussionmentioning

confidence: 99%

Interdependence of plasma membrane nanoscale dynamics of a kinase and its cognate substrate underliesArabidopsisresponse to viral infection

Jolivet,

Deroubaix,

Boudsocq

et al. 2023

Preprint

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Plant viruses represent a risk to agricultural production and as only few treatments exist, it is urgent to identify resistance mechanisms and factors. In plant immunity, plasma membrane (PM)-localized proteins are playing an essential role in sensing the extracellular threat presented by bacteria, fungi or herbivores. Viruses being intracellular pathogens, the role of the plant PM in detection and resistance against viruses is often overlooked. We investigated the role of the partially PM-bound Calcium-dependent protein kinase 3 (CPK3) in viral infection and we discovered that it displayed a specific ability to hamper viral propagation over CPK isoforms that are involved in immune response to extracellular pathogens. More and more evidence support that the lateral organization of PM proteins and lipids underlies signal transduction in plants. We showed here that CPK3 diffusion in the PM is reduced upon activation as well as upon viral infection and that such immobilization depended on its substrate, Remorin (REM1.2), a scaffold protein. Furthermore, we discovered that the viral infection induced a CPK3-dependent increase of REM1.2 PM diffusion. Such interdependence was also observable regarding viral propagation. This study unveils a complex relationship between a kinase and its substrate that contrasts with the commonly described co-stabilisation upon activation while it proposes a PM-based mechanism involved in decreased sensitivity to viral infection in plants.

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Section: Discussionmentioning

confidence: 99%

Interdependence of plasma membrane nanoscale dynamics of a kinase and its cognate substrate underliesArabidopsisresponse to viral infection

Jolivet,

Deroubaix,

Boudsocq

et al. 2023

Preprint

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“…But the sensitivities of these methods cannot meet the need during the latent period of EB (Leiminger, 2009;Latorse et al, 2010;Khan et al, 2018). Because of a lack of proper transcript information that is stably expressed at a high level under a wide range of conditions, it is difficult to ensure the specificity and sensitivity of RNA-based assays (Di Gaspero et al, 2022;Valmonte-Cortes et al, 2022). So, RNA-based methods have not been adopted in past studies.…”

Section: Discussionmentioning

confidence: 99%

Detection of Alternaria solani with high accuracy and sensitivity during the latent period of potato early blight

et al. 2022

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Early blight (EB) disease, caused mainly by Alternaria solani, is an economic threat to potato and tomato production worldwide. Thus, accurate and sensitive detection of the fungal pathogen of this disease in plants at the early infection stage is important for forecasting EB epidemics. In this study, we developed an RNA-based method that enables highly accurate and sensitive A. solani detection in a whole potato leaf at a single spore level based on quantitative real-time polymerase chain reaction (qPCR). We discovered jg1677, a highly expressed gene whose full-length coding sequence is very specific for A. solani, by analyzing A. solani transcripts isolated from enhanced high throughput transcriptome of infected potato leaves by A. solani and using the National Center for Biotechnology Information’s basic local alignment search tool. The specificity of the primers derived from jg1677 was determined using 22 isolates of common potato pathogens, including seven Alternaria isolates. Detecting jg1677 transcripts with qPCR is 1,295 times more sensitive than detecting genomic DNA. In addition, the expression pattern of jg1677 at different infection stages was determined by qPCR. What is more, jg1677 was expressed relatively stable between 15 and 35°C in infected leaves, and its expression was virtually unaffected in isolated leaves left at room temperature for 24 h. Our work provides a much more sensitive and accurate method compared to conditional DNA-based ones, permitting a very early diagnosis of EB and lowering the risk of EB epidemics.

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“…Similarly, the same researchers show further that the infection of Arabidopsis with plantago asiatica mosaic virus induced CPK3-dependent increase of REM1.2 diffusion in the PM, which affected the spread of infection ( Jolivet et al , 2023 ). Notably, the accumulation of AtCPK3 transcripts is significantly increased in response to various viral infections ( Valmonte-Cortes et al , 2022 ), further suggesting an important role of CPK3 in antiviral response regulation. Remorin-related regulation of cell-to-cell transport has also been shown for TuMV.…”

Section: Organelle-specific Regulation Of Ca 2+ Si...mentioning

confidence: 99%

Calcium signaling: an emerging player in plant antiviral defense

Zvereva,

Klingenbrunner,

Teige

2023

Journal of Experimental Botany

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Calcium is a universal messenger in different kingdoms of living organisms and regulates most physiological processes, including defense against pathogens. The threat of viral infections in humans has become very clear in recent years, and this has triggered detailed research into all aspects of host–virus interactions, including the suppression of calcium signaling in infected cells. At the same time, however, the threat of plant viral infections is underestimated in society, and research in the field of calcium signaling during plant viral infections is scarce. Here we highlight an emerging role of calcium signaling for antiviral protection in plants, in parallel with the known evidence from studies of animal cells. Obtaining more knowledge in this domain might open up new perspectives for future crop protection and the improvement of food security.

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The Potential of Molecular Indicators of Plant Virus Infection: Are Plants Able to Tell Us They Are Infected?

Cited by 3 publications

References 73 publications

Interdependence of plasma membrane nanoscale dynamics of a kinase and its cognate substrate underliesArabidopsisresponse to viral infection

Interdependence of plasma membrane nanoscale dynamics of a kinase and its cognate substrate underliesArabidopsisresponse to viral infection

Detection of Alternaria solani with high accuracy and sensitivity during the latent period of potato early blight

Calcium signaling: an emerging player in plant antiviral defense

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