The potential of mixed-species biofilms to address remaining challenges for economically-feasible microalgal biorefineries: A review

Wicker, Rebecca J.; Kwon, Eilhann E.; Khan, Eakalak; Kumar, Vinod; Bhatnagar, Amit

doi:10.1016/j.cej.2022.138481

Cited by 30 publications

(6 citation statements)

References 106 publications

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“…Notably, single-species biofilms seem to have a looser, less-dense appearance (evidenced, for example, by the presence of taller and irregular biofilm structures and streamers; Figure ) as compared to multispecies biofilms. Distinct structural and morphological characteristics in the multispecies biofilms (e.g., dark and regular biofilm flocs in Figure ) could be due to the symbiotic mutualism between various species, even under constrained nutrition and aeration. , Similarly, biofilms grown under long-term and chlorinated conditions are different in structure than short-term multispecies biofilms (Figures and ). This indicates the implications of bacterial species and growth conditions on the structure, composition, and strength of the biofilms.…”

Section: Discussionmentioning

confidence: 99%

Beyond Microbial Inactivation: Unveiling the Potential of Detachment-Promoting Agents in Water Distribution System Biofilm Control

Kabir,

Hasan,

Aggarwal

2024

ACS EST Water

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Biofilms growing on the surfaces of water distribution systems (WDS) pose a significant problem for maintaining the quality of distributed drinking water. Current approaches to biofilm control by inactivating microbial cells (e.g., residual chlorine) involve high concentrations of antimicrobials, which can cause human health hazards, produce harmful byproducts, and still allow appreciable biofilm proliferation on WDS pipes. An alternate paradigm is to instead target the biofilm extracellular polymeric substances (EPS) through "detachment promoting agents" (DPAs) to cause EPS weakening, biofilm dispersal, and eventual detachment. Four potential DPAs (sodium triphosphate [STP], ethylene diamine tetraacetate [EDTA], citrate, and urea) were tested for biofilm detachment at concentrations ranging from 1 to 100 mM for their efficacy in disrupting single-species Staphylococcus epidermidis biofilms and multispecies (microbial consortium derived from WDS) biofilms grown on well plates and in glass capillary flow cells. The protein and polysaccharide contents of detached biofilms in flow cell effluents were also measured. The well-plate study on 24 h-old singlespecies and multispecies biofilms showed significant removal (P-value < 0.002) performance with citrate and STP. Capillary flow cell experiments were conducted with a 100 mM concentration of DPAs on 72 h-old single-species biofilms, 72 h-old multispecies biofilms, and 4-month-old chlorinated multispecies biofilms. From flow cell image analyses, the maximum dispersal for S. epidermidis biofilms (mean ± S.E.) was observed with EDTA (45 ± 13%), while 22−28% mean biofilm removal was observed with citrate, STP, and urea treatment. Multispecies biofilms (both 72 h and 4 months) were more resistant to DPA treatments than single-species S. epidermidis biofilms. The maximum EPS protein in detached biofilm clusters was observed with EDTA treatment, supporting the hypothesis of biofilm dispersal due to EPS weakening. Overall, the results show the effectiveness and early promise of DPAs for biofilm dispersal, offering an additional tool in the biofilm management arsenal.

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Section: Discussionmentioning

confidence: 99%

Beyond Microbial Inactivation: Unveiling the Potential of Detachment-Promoting Agents in Water Distribution System Biofilm Control

Kabir,

Hasan,

Aggarwal

2024

ACS EST Water

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“…C molecules = C 0,molecules − C released molecules (2) where The primary objective of kinetic modeling is to establish a quantitative relationship between the concentration of molecules released into the supernatant and the duration of US (treatment time). This relationship is derived from the solution of Equation (1) as follows:…”

Section: Ultrasonication (Us)mentioning

confidence: 99%

“…Microalgae are a prospective source of valuable nutrients, including pigments, proteins, carbohydrates, and lipid molecules [1]. They use carbon dioxide (CO 2 ) at rates 10-50 times higher than those of terrestrial plants, produce biomass faster than land plants, and have the capacity to remove nitrogen, phosphorus, and heavy metals from water [2]. Despite ongoing research efforts to overcome issues potentially hindering the release of microalgal molecules, efficient downstream processing remains a major challenge for commercial-scale implementation [3].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Tetraselmis suecica Cell Disruption Techniques: Kinetic Study and Extraction of Hydrosoluble Compounds

Rida,

Peydecastaing,

Takache

et al. 2024

Processes

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The optimization of cell disruption is a critical step in microalgal biorefineries. We used the same batch of Tetraselmis suecica culture to compare two mechanical cell disruption techniques, focusing on the extraction yield of water-soluble molecules. The conditions for high-pressure homogenization (HPH) studied were two passes at a moderate pressure of 300 bars. For ultrasound (US) treatment, we used an amplitude of 20% (equivalent to 100 W) for 25 min. These conditions were chosen on the basis of a preliminary screen of extraction conditions. HPH extracted proteins and pigments more efficiently than US, whereas US was superior for uronic acid extraction. Interestingly, the two methods had similar extraction yields for carbohydrates under the studied conditions. We also analyzed the kinetics of molecule release by considering the centrifugation time lag for HPH and applying a first-order kinetic model for US. HPH outperformed US in terms of the immediate extraction and release of molecules.

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“…However, recent studies highlight the importance (and complications) of mimicking a natural ecosystem in such a setup, suggesting the need to arrange and optimise the presence of multiple species together in biological consortia (Natrah et al, 2014;Yao et al, 2019;Mu et al, 2020;Wicker et al, 2023). Reasoning for this includes enhanced metabolism resulting from interactions between different species, growth patterns, and the reproductivity of microalgae.…”

Section: Introductionmentioning

confidence: 99%

Communicating Algae Polycultures: Photobioreactors, the Phycosphere and Its Living Waters

Hendlin,

Weggelaar,

Derossi

et al. 2024

Being Algae

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The potential of mixed-species biofilms to address remaining challenges for economically-feasible microalgal biorefineries: A review

Cited by 30 publications

References 106 publications

Beyond Microbial Inactivation: Unveiling the Potential of Detachment-Promoting Agents in Water Distribution System Biofilm Control

Beyond Microbial Inactivation: Unveiling the Potential of Detachment-Promoting Agents in Water Distribution System Biofilm Control

Comparison of Tetraselmis suecica Cell Disruption Techniques: Kinetic Study and Extraction of Hydrosoluble Compounds

Communicating Algae Polycultures: Photobioreactors, the Phycosphere and Its Living Waters

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