The potential of microdialysis to monitor organic and inorganic nitrogen compounds in soil

Inselsbacher, Erich; Öhlund, Jonas; Jämtgård, Sandra; Huss‐Danell, Kerstin; Näsholm, Torgny

doi:10.1016/j.soilbio.2011.03.003

Cited by 119 publications

(141 citation statements)

References 45 publications

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“…Although this may relate to technical difficulties of sampling amino acids within the rhizosphere (Clode et al, 2009;Inselsbacher et al, 2011), this deficiency is still somewhat surprising as they provide a readily available nutrient source for bacteria (Owens & Jones, 2001;Ryan et al, 2001;Jones et al, 2013;Moe, 2013), and there is increasing evidence of plant uptake of amino acids from the rhizosphere (Jones & Darrah, 1994;Farrar et al, 2003;Sauheitl et al, 2009). Moreover, rhizosphere concentrations of two amino acids, ACC and tryptophan, will affect the capacity of rhizobacteria to influence root ethylene and auxin status, respectively (Dodd et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Rhizobacteria that produce auxins and contain 1‐amino‐cyclopropane‐1‐carboxylic acid deaminase decrease amino acid concentrations in the rhizosphere and improve growth and yield of well‐watered and water‐limited potato (Solanum tuberosum)

Белимов

Dodd

Safronova

et al. 2015

Annals of Applied Biology

129

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Plant-growth-promoting rhizobacteria (PGPR) utilise amino acids exuded from plant root systems, but hitherto there have been no direct measurements of rhizosphere concentrations of the amino acid 1-amino-cyclopropane-1-carboxylic acid (ACC) following inoculation with PGPR containing the enzyme ACC deaminase. When introduced to the rhizosphere of two potato (Solanum tuberosum) cultivars (cv. Swift and cv. Nevsky), various ACC deaminase containing rhizobacteria (Achromobacter xylosoxidans Cm4, Pseudomonas oryzihabitans Ep4 and Variovorax paradoxus 5C-2) not only decreased rhizosphere ACC concentrations but also decreased concentrations of several proteinogenic amino acids (glutamic acid, histidine, isoleucine, leucine, phenylalanine, serine, threonine, tryptophan, tyrosine, valine). These effects were not always correlated with the ability of the bacteria to metabolise these compounds in vitro, suggesting bacterial mediation of root amino acid exudation. All rhizobacteria showed similar root colonisation following inoculation of sand cultures, thus species differences in amino acid utilisation profiles apparently did not confer any selective advantage in the potato rhizosphere. Rhizobacterial inoculation increased root biomass (by up to 50%) and tuber yield (by up to 40%) in pot trials, and tuber yield (by up to 27%) in field experiments, especially when plants were grown under water-limited conditions. Nevertheless, inoculated and control plants showed similar leaf water relations, indicating that alternative mechanisms (regulation of phytohormone balance) were responsible for growth promotion. Rhizobacteria generally increased tuber number more than individual tuber weight, suggesting that accelerated vegetative development was responsible for increased yield.

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Section: Introductionmentioning

confidence: 99%

Rhizobacteria that produce auxins and contain 1‐amino‐cyclopropane‐1‐carboxylic acid deaminase decrease amino acid concentrations in the rhizosphere and improve growth and yield of well‐watered and water‐limited potato (Solanum tuberosum)

Белимов

Dodd

Safronova

et al. 2015

Annals of Applied Biology

129

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“…Soil microdialysis showed that amino acids account for 80% of the soil N supply in undisturbed boreal forest soil, whereas conventional extraction techniques detected inorganic N as the dominant soil N form. Microdialysis and analysis of a larger range of soil organic compounds have great potential to improve knowledge of N and other nutrients (Inselsbacher et al 2011;Warren 2013), as widely used soil extraction methods were established to identify fertiliser needs in agriculture focussed on inorganic nutrients. Promising techniques include high resolution research techniques of soil, roots and soil microorganisms including confocal and nano-SIMS imaging with fluorescent and stable isotope labelled N compounds (Paungfoo- Lonhienne et al 2008;Clode et al 2009;Whiteside et al 2009;Kilburn et al 2010).…”

Section: Organic Nutrients As Sources Of Essential Nutrients For Plantsmentioning

confidence: 99%

The mixotrophic nature of photosynthetic plants

Schmidt¹,

Raven²,

Paungfoo‐Lonhienne³

2013

Functional Plant Biol.

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Abstract. Plants typically have photosynthetically competent green shoots. To complement resources derived from the atmospheric environment, plants also acquire essential elements from soil. Inorganic ions and molecules are generally considered to be the sources of soil-derived nutrients, and plants tested in this respect can grow with only inorganic nutrients and so can live as autotrophs. However, mycorrhizal symbionts are known to access nutrients from organic matter. Furthermore, specialist lineages of terrestrial photosynthetically competent plants are mixotrophic, including species that obtain organic nutrition from animal prey (carnivores), fungal partners (mycoheterotrophs) or plant hosts (hemi-parasites). Although mixotrophy is deemed the exception in terrestrial plants, it is a common mode of nutrition in aquatic algae. There is mounting evidence that non-specialist plants acquire organic compounds as sources of nutrients, taking up and metabolising a range of organic monomers, oligomers, polymers and even microbes as sources of nitrogen and phosphorus. Plasmamembrane located transporter proteins facilitate the uptake of low-molecular mass organic compounds, endo-and phagocytosis may enable the acquisition of larger compounds, although this has not been confirmed. Identifying the mechanisms involved in the acquisition of organic nutrients will provide understanding of the ecological significance of mixotrophy. Here, we discuss mixotrophy in the context of nitrogen and phosphorus nutrition drawing parallels between algae and plants.

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“…Roots of plants are known to exploit such patchiness by preferentially growing in soil macropores (Passioura 1991) and several non-mycorrhizal plant species have been found to produce short-lived root clusters which can be effective at extracting phosphorus when its available concentrations are very low (Lambers et al 2006). Variation of properties such as soil pH, moisture, nutrient content, microbial populations, and allelochemical concentrations in time and space and from point to point along a root system is likely to have profound consequences for plant-plant interactions (Blum 2011), and the importance of developing analytical tools that can sensitively measure such variation without disturbance of the soil has received increasing emphasis (Ens et al 2010;Inselsbacher et al 2011;Rudolph et al 2013).…”

Section: Introductionmentioning

confidence: 99%

Spatial and Temporal Dynamics of Root Exudation: How Important is Heterogeneity in Allelopathic Interactions?

et al. 2014

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Understanding allelopathy has been hindered by the lack of methods available to monitor the dynamics of allelochemicals in the soil. Previous work has demonstrated the feasibility of using polydimethylsiloxane (PDMS) microtubing (silicone tubing microextraction, or STME) to construct sampling devices to monitor the release of lipophilic allelochemicals from plant roots. The objective of this study was to use such sampling devices to intensively monitor thiophene fluxes beneath marigolds over several weeks to gain insight into the magnitude of temporal and spatial heterogeneity in these fluxes. Marigolds were grown in rhizoboxes (20.5 x 20.5 x 3.0 cm) with 16 individual STME samplers per box. Thiophene sampling and HPLC analysis began 45 days after planting. At the end of the study, roots around each sampler were analyzed by HPLC. Results confirmed the tremendous spatial and temporal heterogeneity in thiophene production seen in our previous studies. STME probes show that thiophene concentrations generally increase over time; however, these effects were sampling-port specific. When sampling ports were monitored at 12 h intervals, fluxes at each port ranged from 0 to 2,510 ng day −1 . Fluxes measured over daylight hr averaged 29 % higher than those measured overnight. Fluxes were less than 1 % on average of the total thiophene content of surrounding roots. While the importance of such heterogeneity, or "patchiness", in the root zone has been recognized for soil nutrients, the potential importance in allelopathic interactions has seldom been considered. The reasons for this variability are unclear, but are being investigated. Our results demonstrate that STME can be used as a tool to provide a more finely-resolved picture of allelochemical dynamics in the root zone than has previously been available.

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The potential of microdialysis to monitor organic and inorganic nitrogen compounds in soil

Cited by 119 publications

References 45 publications

Rhizobacteria that produce auxins and contain 1‐amino‐cyclopropane‐1‐carboxylic acid deaminase decrease amino acid concentrations in the rhizosphere and improve growth and yield of well‐watered and water‐limited potato (Solanum tuberosum)

Rhizobacteria that produce auxins and contain 1‐amino‐cyclopropane‐1‐carboxylic acid deaminase decrease amino acid concentrations in the rhizosphere and improve growth and yield of well‐watered and water‐limited potato (Solanum tuberosum)

The mixotrophic nature of photosynthetic plants

Spatial and Temporal Dynamics of Root Exudation: How Important is Heterogeneity in Allelopathic Interactions?

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