The potential of laser interferometry for a non-invasive assessment of biopolymer film structure and biological properties

Biopolymer Matrices Based on Chitosan and Fibroin: A Review Focused on Methods for Studying Surface Properties

“…One of the most modern, reliable and super-resolving methods that is used for applied and fundamental problems of biomedical research, which makes it possible to quickly assess the morphofunctionality of biopolymer coatings, is interference microscopy [99,100]. At the heart of the modern optical method of modulation laser interference microscopy, the local phases of the modulated object of the light wave are measured and taken into account with full control of the polarization of the region, and the obtained interferograms provided on the photodetector after mathematical processing restore the phase portrait of the portrait field of view ( Figure 5) [101,102].…”

“…Obtaining quantitative values of the morphology of living cytoobjects makes it possible to track their change in real-time by obtaining 2D and 3D images. Due to the high speed of measurements and the non-invasiveness of this method, it has taken one of the central positions in the study of the tolerance of cellular objects to the surface of biomedical devices [99,104,105].…”

“…Thus, today, such microscopic methods as atomic force microscopy (AFM), scanning electron microscopy (SEM) and fluorescence and confocal microscopy [106][107][108][109] are known and described in the study of scaffolds based on chitosan and fibroin. The use of interference microscopy [99], tensiometry [110] and ellipsometry [111] are used to study adhesion and adsorption on the surface of the scaffolds. One of the most universal parameters for characterizing the surface and cell adhesion is the value of the surface energy of the material [95].…”