The Potential of High Voltage Discharges for Green Solvent Extraction of Bioactive Compounds and Aromas from Rosemary (Rosmarinus officinalis L.)—Computational Simulation and Experimental Methods

Abstract

Rosemary (Rosmarinus officinalis L.) is a Mediterranean medicinal and aromatic plant widely used due to valuable bioactive compounds (BACs) and aromas. The aim of the study was to evaluate the extraction of intracellular compounds from rosemary combining experimental procedure by means of high voltage electrical discharge (HVED), with a theoretical approach using two computational simulation methods: conductor-like screening model for real solvents and Hansen solubility parameters. The optimal HVED parameters were as follows: frequency 100 Hz, pulse width 400 ns, gap between electrodes 15 mm, liquid to solid ratio 50 mL/g, voltage 15 and 20 kV for argon, and 20 and 25 kV for nitrogen gas. Green solvents were used, water and ethanol (25% and 50%). The comparison was done with modified conventional extraction (CE) extracted by magnetic stirring and physicochemical analyses of obtained extracts were done. Results showed that HVED extracts in average 2.13-times higher total phenol content compared to CE. Furthermore, nitrogen, longer treatment time and higher voltage enhanced higher yields in HVED extraction. HVED was confirmed to have a high potential for extraction of BACs from rosemary. The computational stimulation methods were confirmed by experimental study, ethanol had higher potential of solubility of BACs and aromas from rosemary compared to water.

Recent innovations of ultrasound green technology in herbal phytochemistry: A review

“…While the formation of free-radicals during US processing is considered a main disadvantage of the technology as it affects the bioactivity of components such as phenols [ 83 ], chemical modification such as increasing the extent of hydroxylation of flavonoids [ 84 ] can enhance the antioxidant activity or at least counteract some of the negative effects of free radicals. This led to the fact that optimum US processing conditions where maximum yield and bioactivity could be optimized for various materials.…”

“…Furthermore, the inhibitory effect of α-amylase of the combined technique was increased up to 37% compared to US and HPP with 34% and 29% [ 67 ]. The better outcome achieved by the combined technologies can be explained by the more efficient disruption of plant cell walls by US and the improved diffusion caused by HPP that enhances the release of bioactive compounds compared to their individual potentials [ [83] , [84] , [116] ]. A method combined US with MW confirmed that they could enhance the oil extraction from tea ( Camelia sinensis ) seeds.…”

Ultrasound-Assisted Extraction Optimization of Proanthocyanidins from Kiwi (Actinidia chinensis) Leaves and Evaluation of Its Antioxidant Activity

“…For most plant leaves, the US acoustic cavitation can facilitate the flow of solvents into the plant cells and enhance the desorption of the bioactives from the matrix of solid samples. In addition, the formation of radicals during the optimum conditions of US process can increase the antioxidant activity of the extracted PAs through extension in the hydroxylation process [ 19 ]. This led to the fact that the optimum US conditions for various plant materials are maximizing the extracted yields and their bioactivities.…”

Comparison of GC and NIR spectra as a rapid tool for food fraud detection: Case of butter adulteration with different fat types

“…The PLSR model was applied to determine the potential of predicting the quantitative amount of certain fatty acids. The following parameters were used in the evaluation of the model efficiency: Coefficient of Multiple Determination (R 2 ), Root Mean Square Error of Prediction (RMSEP), Standard Error of Prediction (SEP), and Ratio of Standard Error of Performance to Standard Deviation (RPD) (Nutrizio et al, 2020). Two types of software were used in the data analyses, namely Unscrambler X and XLSTAT.…”