The Potential of Frozen Rumen Fluid with Dimethyl Sulfoxide And Glucose As Cryoprotectant on In Vitro Gas Production And Digestibility

Abstract

As the pressure from the animal welfare community increases, there is an urgent need to find the alternative rumen fluid (RF) source as an essential inoculum for in vitro digestibility studies. The objective of this study is to support the previous studies utilizing fresh RF from abattoir with and without cryoprotectants during the freezing process. A 2x3x3-factorial design was used to ascertain the effectiveness of dimethyl sulfoxide (DMSO) and glucose as cryoprotectants to frozen RF varying from 4, 11, and 18 days to measure the in vitro gas production (IVGP) and digestibility (IVD) of a complete feed consisted of elephant grass and commercial concentrate at 1:1 ratio. factor A being the preservation methods, factor B being the cryoprotectants, and factor C being the preservation time. IVGP shows that the use of DMSO and glucose as cryoprotectants (P1A, P1B, P2A, & P2B) only held back quality performance and made the noncryoprotected frozen RF (P0A) is the most applicable method for preservation that lasts until 18 days. IVD however resulted in the most similar frozen RF treatment with gradual freezing that uses DMSO as cryoprotectant (P1B). Thus, making DMSO and glucose influenced nothing significant to the frozen RF. Maintaining the reduction of animal welfare while simultaneously keeping the data variation low is achievable and efficient.