2021
DOI: 10.1007/s00216-020-03066-w
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The potential of fluorescent dyes—comparative study of Nile red and three derivatives for the detection of microplastics

Abstract: During the last years, microplastics in the environment came to the fore in environmental science research. For an appropriate risk assessment, it is essential to know the levels of microplastic contamination in the environment. In the field of microplastic detection, extensive research has been carried out in recent years. While common methods such as Raman spectroscopy and pyrolysis GC-MS are time-consuming and require trained staff and expensive equipment, there is the need for a cheap and easily applicable… Show more

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Cited by 60 publications
(41 citation statements)
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“…In most cases, Nile Red, able to bind to different polymers leading to colors from yellow to red depending on the plastic’s hydrophilicity, has been exploited as a fluorescent probe for the visual inspection and categorization of different MP [ 20 , 21 , 22 , 23 , 24 ]. Nile Red derivatives [ 25 , 26 ] have been presented as possible candidates able to improve the water solubility and selectivity of Nile Red [ 27 ]. Nevertheless, there are very few examples of other fluorescent probes.…”
Section: Introductionmentioning
confidence: 99%
“…In most cases, Nile Red, able to bind to different polymers leading to colors from yellow to red depending on the plastic’s hydrophilicity, has been exploited as a fluorescent probe for the visual inspection and categorization of different MP [ 20 , 21 , 22 , 23 , 24 ]. Nile Red derivatives [ 25 , 26 ] have been presented as possible candidates able to improve the water solubility and selectivity of Nile Red [ 27 ]. Nevertheless, there are very few examples of other fluorescent probes.…”
Section: Introductionmentioning
confidence: 99%
“…For the detection of PA particles, the fluorescent dye Nile Red (Carl Roth) was chosen, which can be used to selectively stain polymers [35,36]. The polymer-specific fluorescence facilitates the differentiation between microplastic particles and organic as well as inorganic substances.…”
Section: Detection Of Microplastics In Chaoborus Spmentioning
confidence: 99%
“…Powder of PENPs was loaded on glass slides and stained with a few drops of 1 mg/ml Nile red dye in acetone. Slides were kept in the dark box for 20 mins before fluorescence microscope examination (Carl Zeiss Microscopy GmbH, Jena, Germany) using epifluorescence mode ( 40 objective lens) with Alexa Fluor 660 filter for red fluorescence color (with excitation at bandpass (BP) 600±50 nm; emission BP 685±50 nm) (Sturm et al, 2021).…”
Section: Characterization Of Pe-npsmentioning
confidence: 99%