The potential of Cycloclasticus and Altererythrobacter strains for use in bioremediation of petroleum-aromatic-contaminated tropical marine environments

Teramoto, Maki; Suzuki, Masahito; Hatmanti, Ariani; Harayama, Shigeaki

doi:10.1016/j.jbiosc.2009.12.008

Cited by 45 publications

(22 citation statements)

References 32 publications

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“…2); although they shared a relatively high degree of 16S rRNA gene sequence similarity to members of AG1 (96%), the extent of sequence divergence implied two separate groups of anthracene-degrading organisms. Members of the Altererythrobacter genus have been only rarely associated with PAH degradation, with one strain isolated from Indonesian seawater shown to grow on the 3-ring PAHs phenanthrene and fluorene; growth on anthracene was not tested in that study (Teramoto et al, 2010). Members of this genus were undetectable at most time points in pyrosequence libraries of the untreated soil and bioreactor-treated soil over the first several months of operation , so their dominant presence in 13 C-enriched DNA clone libraries coupled with their low relative abundance in bioreactor slurry (estimated 0.1% of total 16S rRNA genes) may indicate a specific affinity for anthracene as a growth substrate.…”

Section: Discussionmentioning

confidence: 96%

Biostimulation Reveals Functional Redundancy of Anthracene-Degrading Bacteria in Polycyclic Aromatic Hydrocarbon-Contaminated Soil

Singleton²,

Aitken³

2013

Environmental Engineering Science

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Stable-isotope probing was previously used to identify bacterial anthracene-degraders in untreated soil from a former manufactured gas plant site. However, subsequent pyrosequence analyses of total bacterial communities and quantification of 16S rRNA genes indicated that relative abundances of the predominant anthracene-degrading bacteria (designated Anthracene Group 1) diminished as a result of biological treatment conditions in lab-scale, aerobic bioreactors. This study identified Alphaproteobacterial anthracene-degrading bacteria in bioreactor-treated soil which were dissimilar to those previously identified. The largest group of sequences was from the Alterythrobacter genus while other groups of sequences were associated with bacteria within the order Rhizobiales and the genus Bradyrhizobium. Conditions in the bioreactor enriched for organisms capable of degrading anthracene which were not the same as those identified as dominant degraders in the untreated soil. Further, these data suggest that identification of polycyclic aromatic hydrocarbon-degrading bacteria in contaminated but untreated soil may be a poor indicator of the most active degraders during biological treatment.

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Section: Discussionmentioning

confidence: 96%

Biostimulation Reveals Functional Redundancy of Anthracene-Degrading Bacteria in Polycyclic Aromatic Hydrocarbon-Contaminated Soil

Singleton²,

Aitken³

2013

Environmental Engineering Science

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“…2A75 ( = NBRC 105763; [20]) and Altererythrobacter sp. 1 l E25 ( = NBRC 105748; [18]) were obtained from NBRC (NITE Biological Resource Center), while Erythrobacter aquimaris JCM 12189 T was from RIKEN BRC through the National Bio-Resource Project of the MEXT, Japan.…”

Section: Methodsmentioning

confidence: 99%

“…2A75 [20] and an aromatic-hydrocarbon degrader Altererythrobacter sp. 1 l E25 [18]) freshly grown on a dMB plate supplemented with 0.5% (w/v) pyruvate at 20°C were collected and suspended in filter-sterilized seawater to an OD 600 of 0.5. Then 150 µl of this bacterial suspension was inoculated into 3 ml sterilized SW medium supplemented with 3 µl crude oil (heat-treated Arabian light crude oil; [20]).…”

Section: Methodsmentioning

confidence: 99%

Specialized Hydrocarbonoclastic Bacteria Prevailing in Seawater around a Port in the Strait of Malacca

2013

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Major degraders of petroleum hydrocarbons in tropical seas have been indicated only by laboratory culturing and never through observing the bacterial community structure in actual environments. To demonstrate the major degraders of petroleum hydrocarbons spilt in actual tropical seas, indigenous bacterial community in seawater at Sentosa (close to a port) and East Coast Park (far from a port) in Singapore was analyzed. Bacterial species was more diverse at Sentosa than at the Park, and the composition was different: γ-Proteobacteria (57.3%) dominated at Sentosa, while they did not at the Park. Specialized hydrocarbonoclastic bacteria (SHCB), which use limited carbon sources with a preference for petroleum hydrocarbons, were found as abundant species at Sentosa, indicating petroleum contamination. On the other hand, SHCB were not the abundant species at the Park. The abundant species of SHCB at Sentosa were Oleibacter marinus and Alcanivorax species (strain 2A75 type), which have previously been indicated by laboratory culturing as important petroleum-aliphatic-hydrocarbon degraders in tropical seas. Together with the fact that SHCB have been identified as major degraders of petroleum hydrocarbons in marine environments, these results demonstrate that the O. marinus and Alcanivorax species (strain 2A75 type) would be major degraders of petroleum aliphatic hydrocarbons spilt in actual tropical seas.

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“…Although generally described as aerobic, it has been previously identified in anaerobic oil-degrading consortia (Zhang et al 2011). Oil-degrading species of Rhodovulum were isolated recently by Teramoto et al (2010), whereas species of Brevundimonas and Stappia have also been characterized as oil-degraders (Al-Awadhi et al 2007;Chaîneau et al 1999).…”

Section: Alphaproteobacteriamentioning

confidence: 99%

Compositions of microbial communities associated with oil and water in a mesothermic oil field

Kryachko

Dong

Sensen

et al. 2011

Antonie van Leeuwenhoek

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Samples of produced water and oil obtained from the Enermark field (near Medicine Hat, Alberta, Canada) were separated into oil and aqueous phases first gravitationally and then through centrifugation at 20°C in an atmosphere of 90% N(2) and 10% CO(2). Biomass that remained associated with oil after gravitational separation (1×g) was dislodged by centrifugation at 25,000×g. DNA was isolated from the aqueous and oil-associated biomass fractions and subjected to polymerase chain reaction amplification with primers targeting bacterial and archaeal 16S rRNA genes. DNA pyrosequencing and bioinformatics tools were used to characterize the resulting 16S rRNA gene amplicons. The oil-associated microbial community was less diverse than that of the aqueous phase and had consistently higher representation of hydrogenotrophs (methanogens of the genera Methanolobus and Methanobacterium and acetogens of the genus Acetobacterium), indicating the oil phase to be a primary source of hydrogen. Many known hydrocarbon degraders were also found to be oil-attached, e.g. representatives of the gammaproteobacterial genus Thalassolituus, the actinobacterial genus Rhodococcus and the alphaproteobacterial genera Sphingomonas, Brevundimonas and Stappia. In contrast, all eight representatives of genera of the Deltaproteobacteria identified were found to be associated with the aqueous phase, likely because their preferred growth substrates are mostly water-soluble. Hence, oil attachment was seen for genera acting on substrates found primarily in the oil phase.

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The potential of Cycloclasticus and Altererythrobacter strains for use in bioremediation of petroleum-aromatic-contaminated tropical marine environments

Cited by 45 publications

References 32 publications

Biostimulation Reveals Functional Redundancy of Anthracene-Degrading Bacteria in Polycyclic Aromatic Hydrocarbon-Contaminated Soil

Biostimulation Reveals Functional Redundancy of Anthracene-Degrading Bacteria in Polycyclic Aromatic Hydrocarbon-Contaminated Soil

Specialized Hydrocarbonoclastic Bacteria Prevailing in Seawater around a Port in the Strait of Malacca

Compositions of microbial communities associated with oil and water in a mesothermic oil field

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