The POTENTIAL OF BUTTERFLY PEA FLOWER METHANOL EXTRACT AS AN ANTIOXIDANT BY IN SILICO

Abstract

Butterfly pea flower (Clitoria ternatea L.) is a flowering plant from the Fabecea family that can grow vines. Butterfly pea flower are known to have chemical components that can act as antioxidants. This study aims to predict the potential of active compounds from methanol extract of butterfly pea flower in inhibiting reactive oxygen species (ROS) based on bond affinity (∆G), the value of Root Mean Square Deviation (RMSD) and their interactions. The method used was a computational method with in silico technique. The software used was Autodock Vina with visualization using the Biovia Discovery Studio Visualizer 2020. The enzyme receptor was NADPH Oxidase (NOX) obtained from Protein Data Bank and the test ligands were a chemical compound from methanol extract of butterfly pea flower. The results of the in silico study showed that the NO had a innate ligand, namely the GTP ligand which has a ∆G value of -7.3 kcal/mol, an RMSD value of 3.1111 Å and the interaction with the receptor that involves the presence of hydrogen bonds. Based on the results of the analysis of 11 test ligands, the chemical component of caffeine was predicted to have the most potential in inhibiting ROS compounds with a value of ∆G -5.4, RMSD value of 1.328 Å and had the same amino acid residue in hydrogen bonding, namely ASP118, and GLY15. The test ligand had the ability to inhibit ROS compounds with a lower level of stability than the innate ligand.
 Keywords: antioxidant; butterfly pea flower; in silico; NADPH oxidase, ROS

Antioxidant Activity of TEMON (Clitoria ternatea and Citrus sp.) as an Infused Herbal Tea

“…The assay was done triplicate for each treatment. protect canine erythrocytes from hemolysis and oxidative damage induced by 2,2′-azobis-2methyl-propanimidamide dihydrochloride (AAPH) (Fitrilia et al, 2020). Methanol extract of the butterfly pea flower compound as an antioxidant in inhibiting ROS and the most potential to inhibit ROS, was caffeine (Fitrilia et al, 2020).…”

“…protect canine erythrocytes from hemolysis and oxidative damage induced by 2,2′-azobis-2methyl-propanimidamide dihydrochloride (AAPH) (Fitrilia et al, 2020). Methanol extract of the butterfly pea flower compound as an antioxidant in inhibiting ROS and the most potential to inhibit ROS, was caffeine (Fitrilia et al, 2020). DPPH scavenging activity methanol extract was more active than ethanol extracts (IC50 values were 1 mg/mL and 4 mg/mL, respectively) (Kamkaen, and Wilkinson, 2009).…”

“…Butterfly pea flowers are used as ornamental plants because of their attractive colors. Telang have various name, depending on the region such as blue flowers, teleng flowers for Java, talang flowers for Sulawesi and bisi for Maluku, kelenit flowers and telang flowers for Sumatra (Fitrilia et al, 2020). One of the unique characteristics of butterfly pea flower is bluishpurple at a low acidic condition, The combination of exotic color and health benefits will promote telang as functional drink (Marpaung et al, 2020).…”

Study of nonlinear optical responses of phytochemicals of Clitoria ternatea by quantum mechanical approach and investigation of their anti-Alzheimer activity with in silico approach

“…A review of the neuropharmacological potentiality of Clitorin is explained in detail in the cited paper [23,24]. It is also used as an anti-oxidant [25]. It is also used as a base supplement for eye drops [26].…”

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“…The grid box was constructed based on the dimensions mentioned above, and molecular docking was carried out using Perl. The results obtained were analyzed to determine the binding energy and the interacting atoms between protein and the ligand and were visualized using bio via discovery studio software 17 .…”