The potential of aquatic bloodfeeding and nonbloodfeeding leeches as a tool for iDNA characterisation

Lynggaard, Christina; Oceguera‐Figueroa, Alejandro; Kvist, Sebastian; Gilbert, M. Thomas P.; Bohmann, Kristine

doi:10.1111/1755-0998.13486

Cited by 15 publications

(11 citation statements)

References 102 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While eDNA-based approaches may allow for relatively rapid sampling, it often requires weeks or months of laboratory work, and species identifications can be limited by primer biases and DNA reference databases. Moreover, only freshwater eDNA (Ushio et al, 2018) and iDNA from bulk arthropod samples and leeches (Ji et al, 2022;Lynggaard et al, 2019;Lynggaard, Oceguera-Figueroa, et al, 2022) have been found applicable for bird detection.…”

Section: Vertebrate Taxa Detected Using Airborne Ednamentioning

confidence: 99%

Airborne environmental DNA captures terrestrial vertebrate diversity in nature

Lynggaard

Frøslev

Johnson

et al. 2023

Molecular Ecology Resources

Self Cite

View full text Add to dashboard Cite

The current biodiversity and climate crises highlight the need for efficient tools to monitor terrestrial ecosystems. Here, we provide evidence for the use of airborne eDNA analyses as a novel method for detecting terrestrial vertebrate communities in nature. Metabarcoding of 143 airborne eDNA samples collected during 3 days in a mixed forest in Denmark yielded 64 bird, mammal, fish and amphibian taxa, of which the detected 57 ‘wild’ taxa represent over a quarter of the around 210 terrestrial vertebrates that occur in the overall area. We provide evidence for the spatial movement and temporal patterns of airborne eDNA and for the influence of weather conditions on vertebrate detections. This study demonstrates airborne eDNA for high‐resolution biomonitoring of vertebrates in terrestrial systems and elucidates its potential to guide global nature management and conservation efforts in the ongoing biodiversity crisis.

show abstract

Section: Vertebrate Taxa Detected Using Airborne Ednamentioning

confidence: 99%

Airborne environmental DNA captures terrestrial vertebrate diversity in nature

Lynggaard

Frøslev

Johnson

et al. 2023

Molecular Ecology Resources

Self Cite

View full text Add to dashboard Cite

show abstract

“…According to their diet, leeches are divided into 3 groups: carnivorous, blood-sucking and liquid-sucking groups (Sawyer, 1986;Lynggaard et al, 2022). The leeches we studied also belong to the above groups.…”

Section: Feeding Of Leeches and Gastropodsmentioning

confidence: 99%

Ecology of Leeches and Gastropods of the Lower Ak-Buura River, Fergana Valley, Uzbekistan

Solijonov

Umarov

2022

Bull. Iraq nat. Hist. Mus.

View full text Add to dashboard Cite

This study was conducted on species composition, morphology, ecological characteristics, biotope distribution, ecological groups, biodiversity indicators and zoogeography of leeches and gastropods distributed in the lower Ak-Buura River. According to the results, it was found that 7 species of leeches belonging to 4 families and 6 genera and 10 species of fresh-water gastropods belonging to 3 families and 6 genera live in the lower Ak-Buura River. In the river, it was observed that leeches are mainly distributed in muddy biotopes, and gastropods are widespread in muddy, stony and sandy biotopes with a lot of plants. Biodiversity indices of leeches and gastropods in the Ak-Buura River were analyzed using the Shannon index. As a result, it was determined that the biodiversity index of the Ak-Buura river is lower than other rivers in the Fergana valley (H' = 0.81-1.17). This is mainly due to the eutrophication of some parts of the river and the instability of the water hydro-regime. Most of the leeches distributed in the river are carnivores, and gastropods are phytophages. According to the zoogeographical analysis, it was found that leeches are Holarctic, Palearctic, Western Palearctic, and gastropods are composed of European-Siberian, Palearctic and Central Asian species.

show abstract

“…Like eDNA, invertebrate-derived DNA (iDNA) often allows for the detection of vertebrate mtDNA. Leeches (e.g., Drinkwater, Jucker, et al, 2021;Lynggaard, Oceguera-Figueroa, et al, 2022;Schnell et al, 2012Schnell et al, , 2015Schnell et al, , 2018, carrion flies (e.g., Calvignac-Spencer, Merkel, et al, 2013;Gogarten et al, 2020;Hoffmann et al, 2018;Rodgers et al, 2017), sandflies (Kocher et al, 2017;Massey et al, 2022), mosquitos (e.g., Kocher et al, 2017;Massey et al, 2022), and dung beetles (Drinkwater, Williamson, et al, 2021) have all been successfully used in metabarcoding studies targeting mtDNA.…”

Section: Introductionmentioning

confidence: 99%

Mammal mitogenomics from invertebrate‐derived DNA

Danabalan,

Merkel,

Bærholm Schnell

et al. 2023

Environmental DNA

View full text Add to dashboard Cite

The metabarcoding of vertebrate DNA found in invertebrate‐derived DNA (iDNA) has proven a powerful tool for monitoring biodiversity. To date, iDNA has primarily been used to detect the presence/absence of particular taxa using metabarcoding, though recent efforts demonstrated the potential utility of these data for estimating relative animal abundance. Here, we test whether iDNA can also be used to reconstruct complete mammalian mitogenomes and therefore bring the field closer to population‐level analyses. Specifically, we used mitogenomic hybridization capture coupled with high‐throughput sequencing to analyze individual (N = 7) or pooled (N = 5) fly‐derived DNA extracts, and individual (N = 7) or pooled (N = 1) leech‐derived DNA extracts, which were known a priori to contain primate DNA. All sources of iDNA showed their ability to generate large amounts of mammalian mitogenomic information and deeper sequencing of libraries is predicted to allow for even more complete recovery of primate mitogenomes from most samples (90%). Sixty percent of these iDNA extracts allowed for the recovery of (near) complete mammalian mitochondrial genomes (hereafter mitogenomes) that proved useable for phylogenomic analyses. These findings contribute to paving the way for iDNA‐based population mitogenomic studies of terrestrial mammals.

show abstract

The potential of aquatic bloodfeeding and nonbloodfeeding leeches as a tool for iDNA characterisation

Cited by 15 publications

References 102 publications

Airborne environmental DNA captures terrestrial vertebrate diversity in nature

Airborne environmental DNA captures terrestrial vertebrate diversity in nature

Ecology of Leeches and Gastropods of the Lower Ak-Buura River, Fergana Valley, Uzbekistan

Mammal mitogenomics from invertebrate‐derived DNA

Contact Info

Product

Resources

About