The Potential of Algal Biotechnology to Produce Antiviral Compounds and Biopharmaceuticals

Rosales‐Mendoza, Sergio; García-Silva, Ileana; González‐Ortega, Omar; Sandoval-Vargas, José M.; Malla, Ashwini; Vimolmangkang, Sornkanok

doi:10.3390/molecules25184049

Cited by 53 publications

(44 citation statements)

References 213 publications

(219 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The demand and use of recombinant proteins have become powerful tools globally due to their wide significance and important application in industry, diagnosis, and therapeutics ( Rosales-Mendoza et al, 2020a ). Hence, there is an immense need for the development and production of recombinant proteins in large quantities using various eukaryotic expression systems to provide several solutions, such as cost reduction and bioavailability of these biological substances in low- and middle-income countries in a safer and efficient way, by expanding the potential biosystems ( Rosales-Mendoza et al, 2020b ).…”

Section: Discussionmentioning

confidence: 99%

Efficient Transient Expression of Recombinant Proteins Using DNA Viral Vectors in Freshwater Microalgal Species

et al. 2021

Self Cite

View full text Add to dashboard Cite

The increase in the world population, the advent of new infections and health issues, and the scarcity of natural biological products have spotlighted the importance of recombinant protein technology and its large-scale production in a cost-effective manner. Microalgae have become a significant promising platform with the potential to meet the increasing demand for recombinant proteins and other biologicals. Microalgae are safe organisms that can grow rapidly and are easily cultivated with basic nutrient requirements. Although continuous efforts have led to considerable progress in the algae genetic engineering field, there are still many hurdles to overcome before these microorganisms emerge as a mature expression system. Hence, there is a need to develop efficient expression approaches to exploit microalgae for the production of recombinant proteins at convenient yields. This study aimed to test the ability of the DNA geminiviral vector with Rep-mediated replication to transiently express recombinant proteins in the freshwater microalgal species Chlamydomonas reinhardtii and Chlorella vulgaris using Agrobacterium-mediated transformation. The SARS-CoV-2 receptor binding domain (RBD) and basic fibroblast growth factor (bFGF) are representative antigen proteins and growth factor proteins, respectively, that were subcloned in a geminiviral vector and were used for nuclear transformation to transiently express these proteins in C. reinhardtii and C. vulgaris. The results showed that the geminiviral vector allowed the expression of both recombinant proteins in both algal species, with yields at 48 h posttransformation of up to 1.14 μg/g RBD and 1.61 ng/g FGF in C. vulgaris and 1.61 μg/g RBD and 1.025 ng/g FGF in C. reinhardtii. Thus, this study provides a proof of concept for the use of DNA viral vectors for the simple, rapid, and efficient production of recombinant proteins that repress the difficulties faced in the genetic transformation of these unicellular green microalgae. This concept opens an avenue to explore and optimize green microalgae as an ideal economically valuable platform for the production of therapeutic and industrially relevant recombinant proteins in shorter time periods with significant yields.

show abstract

Section: Discussionmentioning

confidence: 99%

Efficient Transient Expression of Recombinant Proteins Using DNA Viral Vectors in Freshwater Microalgal Species

et al. 2021

Self Cite

View full text Add to dashboard Cite

show abstract

“…Microalgae of the genus Chlorella find a plethora of current and potential uses. Many hopes are lain in their ability to convert sewage into useful materials ( Liu and Chen, 2016 ; Markou et al, 2018 ), to constitute a source of renewable fuel ( Wang et al, 2020 ) or—at lower volumes but higher value—of a nonsynthetic source of chemicals, not at least food or feed additives such as polyunsaturated fatty acids ( Haslam et al, 2020 ; Madhubalaji et al, 2020 ), carotenoids ( Sun et al, 2019 ; Zhang et al, 2020 ), or many other pharmaceutically valuable compounds ( Rosales-Mendoza et al, 2020 ). Chlorella is regarded as a potential future source of protein for direct consumption, as well as use in livestock farming ( Amorim et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

The Structural Difference of Isobaric N-Glycans of Two Microalgae Samples Reveals Taxonomic Distance

et al. 2021

View full text Add to dashboard Cite

Microalgae of the Chlorella clade are extensively investigated as an environmentally friendly source of renewable biofuels and high-value nutrients. In addition, essentially unprocessed Chlorella serves as wholesome food additive. A recent study on 80 commercial Chlorella preparations revealed an unexpected variety of protein-linked N-glycan patterns with unprecedented structural features, such as the occurrence of arabinose. Two groups of products exhibited a characteristic major N-glycan isobaric to the Man2GlcNAc2XylFuc N-glycan known from pineapple stem bromelain, but tandem mass spectrometry (MS/MS) analysis pointed at two types of N-glycan different from the bromelain structure, as well as from each other. Here we report the exact structures of these two novel N-glycan structures, elucidated by nuclear magnetic resonance spectroscopy and MS/MS, as well as on their phylogenetic context. Despite their humble size, these two N-glycans exhibited a very different design with structural features unrelated to those recently described for other Chlorella-clade strains. The major glycans of this study presented several novel structural features such as substitution by arabinose or xylose of the internal N-acetylglucosamine, as well as methylated sugars. ITS1-5.8S-ITS2 rDNA barcode analyses revealed that the xylose-containing structure derived from a product primarily comprising Scenedesmus species, and the arabinose-containing glycan type related to Chlorella species (SAG211-34 and FACHB-31) and to Auxenochlorella. This is another example where characteristic N-glycan structures distinguish phylogenetically different groups of microalgae.

show abstract

“…A comparison of different algae-derived compounds[9,18].Significantly inhibit the acute infection of MT4 cells and the chronic infection of H9 cells with HIV-1 • Sulfated form of alginate i.e. sulfated polymannuroguluronate (SPMG) inhibits HIV-1 infection Galactans, from red algae Agardhiella tenera, Schizymenia binderi, Brazilian marine alga Cryptonemia crenulata and marine alga Callophyllis variegate • Pigments Phycobilins from cyanobacteria like Oscillatoria sp., Nostoc sp., Anabaena sp.…”

mentioning

confidence: 99%

Can algae contribute to the war with Covid-19?

et al. 2021

View full text Add to dashboard Cite

The world at large is facing a new threat with the emergence of the Coronavirus Disease 2019 (COVID-19) pandemic. Though imperceptible by the naked eye, the medical, sociological and economical implications caused by this newly discovered virus have been and will continue to be a great impediment to our lives. This health threat has already caused over two million deaths worldwide in the span of a year and its mortality rate is projected to continue rising. In this review, the potential of algae in combating the spread of COVID-19 is investigated since algal compounds have been tested against viruses and algal anti-inflammatory compounds have the potential to treat the severe symptoms of COVID-19. The possible utilization of algae in producing valueadded products such as serological test kits, vaccines, and supplements that would either mitigate or hinder the continued health risks caused by the virus is prominent. Many of the characteristics in algae can provide insights on the development of microalgae to fight against SARS-CoV-2 or other viruses and contribute in manufacturing various green and high-value products.

show abstract

The Potential of Algal Biotechnology to Produce Antiviral Compounds and Biopharmaceuticals

Cited by 53 publications

References 213 publications

Efficient Transient Expression of Recombinant Proteins Using DNA Viral Vectors in Freshwater Microalgal Species

Efficient Transient Expression of Recombinant Proteins Using DNA Viral Vectors in Freshwater Microalgal Species

The Structural Difference of Isobaric N-Glycans of Two Microalgae Samples Reveals Taxonomic Distance

Can algae contribute to the war with Covid-19?

Contact Info

Product

Resources

About