The potential of acetylsalicylic acid and vitamin E in modulating inflammatory cascades in chickens under lipopolysaccharide-induced inflammation

Abstract

Distinct enzymes, including cyclooxygenase 1 and 2 (COX-1 and COX-2), lipoxygenase (LOXs), and cytochrome P450 monooxygenase (CYP450), produce different stress mediators and mediate inflammation in birds. Bioactive agents such as acetylsalicylic acid (ASA) and vitamin E (vE) may affect enzyme activities and could be used in poultry production to control the magnitude of acute phase inflammation. Here, we characterized COX, LOX, and CYP450 mRNA expression levels in chicken immune tissues in response to Escherichia coli lipopolysaccharide (LPS) challenge and investigated whether ASA and vE could alter gene expression. Additionally, for the first time in chickens, we evaluated oxygen consumption by platelet mitochondria as a biomarker of mitochondria function in response to ASA- and vE. LPS challenge compromised bird growth rates, but neither dietary ASA nor vE significantly ameliorated this effect; however, gradually increasing dietary vE levels were more effective than basal levels. ASA regulated arachidonic acid metabolism, providing an eicosanoid synthesis substrate, whereas gradually increasing vE levels evoked aspirin resistance during challenge. Gene expression in immune tissues was highly variable, indicating a complex regulatory network controlling inflammatory pathways. However, unlike COX-1, COX-2 and CYP450 exhibited increased mRNA expression in some cases, suggesting an initiation of novel anti-inflammatory and pro-resolving signals during challenge. Measuring oxygen consumption rate, we revealed that neither the ASA nor vE levels applied here exerted toxic effects on platelet mitochondria.

Cannabis-derived cannabidiol and nanoselenium improve gut barrier function and affect bacterial enzyme activity in chickens subjected to C. perfringens challenge

“…Gut samples collected from 23-day-old chickens were additionally analysed by RT-PCR to measure the mRNA expression levels of GLP2, HSP70, TLR4, JAM2, ZO-1, and TFF2. RNA was isolated from tissue according to the protocol described by Konieczka et al [ 24 ] A GeneMATRIX Universal RNA Purification Kit (EURX Ltd., Gdańsk, Poland) was used to isolate total RNA.…”

Anti-Apoptotic Effect of Apelin in Human Placenta: Studies on BeWo Cells and Villous Explants from Third-Trimester Human Pregnancy

“…The rate of change in fluorescence signal per minute was calculated for each 10 min interval, and an average signal change per minute during the whole 90 min period was calculated for each cell line. Traces of fluorescence were transformed into oxygen concentration profiles based on the work of Konieczka et al [57], using the following Equation (1):…”

The effect of different dietary ratios of lysine and arginine in diets with high or low methionine levels on oxidative and epigenetic DNA damage, the gene expression of tight junction proteins and selected metabolic parameters in Clostridium perfringens-challenged turkeys

“…RNA isolation was performed in accordance with previously developed procedures with some modifications [25]. Briefly; total RNA from the ilium was isolated using the GeneMATRIX Universal RNA Purification Kit (EURX Ltd., Gdańsk, Poland) according to the manufacturer's protocol.…”