The potential impact of exotic Lymnaea spp. on fascioliasis in Australasia

Boray, J. C.

doi:10.1016/0304-4017(78)90004-3

Cited by 95 publications

(78 citation statements)

References 15 publications

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“…Problems of compatibility between snail strain and F. hepcdica have already been studied by Boray (1969Boray ( , 1978 who showed that prevalence and cercaria pro duction can be modified according to the strain's ori gin. Some variation in the prevalence of F. bepalica (41 % to 51 % at day 30 of experiment) and the total number of rediae (18 to 22 per snail) has also been noted by Rondelaud and Barthe (1982) in the same colony of L. truncatula exposed to different strains of miracidia that originated from cattle from three geographical origins.…”

Section: Discussionmentioning

confidence: 99%

Fasciola hepatica: The influence of the definitive host on the characteristics of infection in the snailLymnaea truncatula

Rondelaud¹,

Dreyfuss

1995

Parasite

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Summary:Investigations were carried out in Lymnaea truncatula to determine whether redial burden and shedding of F. hepatica cercariae vary when the same population of Lymnaea truncatula is exposed to miracidia that hatched from eggs collected in different naturally infected definitive hosts (cattle, sheep, and rabbits originating from the same region). Snails measuring 4 mm in height wete each exposed to two miracidia hatched from these eggs, and were then raised at 20 °C for 30 days. The prevalence of infected snails was 35 %, 66 %, and 86 % in the groups exposed to miracidia originating from rabbits, sheep, and cattle respectively. Cercariae were shed from snails of the three groups, however, shedding varied greatly depending on the origin of the miracidia. The total number of cercariae was significantly decreased in the group infected with miracidia from rabbits than in the others (54.5 per snail vs 1 57.8 and 21 6.4). The arcadian rhythm in the shedding of cercariae was also changed in the former group, with maximal shedding between 4 a.m. and 8 a.m. No significant difference was noted in the other characteristics of snail infection between the three groups. Histological examination of infected snails revealed that the total number of rediae was significantly decreased at day 30 in the snails infected from miracidia that originated from rabbits than in the other groups (a mean of 2 1 .3 vs 38.4 and 43.7). Degenerated, free rediae were most numerous in the former group than in the two others. The definitive host species may play a role in the development of Fasciola infection in the snail by limiting redial and cercarial burdens. From this study, trematode eggs collected in cattle or sheep are more efficient for transmission of the disease than those obtained from rabbits.

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Section: Discussionmentioning

confidence: 99%

Fasciola hepatica: The influence of the definitive host on the characteristics of infection in the snailLymnaea truncatula

Rondelaud¹,

Dreyfuss

1995

Parasite

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“…natalen-sis has, in fact, been recognized by Boray (1978) as a species that has a normal parasite-host relationship. It should be remembered, however, that F. gigantica has only recently been detected into Madagascar (Daynès, I960), and that infection due to the parasite has spread over the entire island and has become a major problem to the livestock industry (Touratier, 1988).…”

Section: (1983)mentioning

confidence: 99%

Several observations concerning cercarial sheddings ofFasciola giganticafromLymnaea natalensis

Costa¹,

Dreyfuss²,

Rakotondravao³

et al. 1994

Parasite

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“…Galba truncatula is the main lymnaeid intermediate host in the Old World (Taylor, 1965;Torgerson and Claxton, 1999) but most of the other lymnaeid species of Western Europe may also sustain the larval development of the liver fluke if they are exposed to miracidia during their first days of life (Boray, 1978). For example, Radix labiata was found to act as an alternative intermediate host of F. hepatica, at least under laboratory conditions, because it harboured the intra-molluscan development and allowed the subsequent shedding of cercariae.…”

Section: Introductionmentioning

confidence: 99%

An optimized DNA extraction and multiplex PCR for the detection of Fasciola sp. in lymnaeid snails

Caron

Righi²,

Lempereur

et al. 2011

Veterinary Parasitology

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Phenol/Chloroform/Proteinase K Multiplex PCR Epidemiology a b s t r a c t This study deals with the development and validation of an original PCR protocol to assess the presence of Fasciola hepatica in Galba truncatula its main intermediate host in Western Europe. In the present study two DNA extraction techniques are compared and a new multiplex PCR is described. The Chelex ® DNA extraction technique showed to be more appropriate than the classical Phenol/Chloroform/Proteinase K based method because of the absence of toxic organic solvent, shorter duration and lower cost, and a higher reproducibility regarding DNA concentrations and wavelength ratios. The multiplex PCR was set up to amplify the lymnaeid internal transcribed spacer 2 sequence (500-600 bp) that act as an internal control and a 124 bp Fasciola sp. sequence that is repeated more than 300,000 times in fluke whole genome. Ninety six snails were pooled and 6 snails (6.25%) found positive for Fasciola sp. The limit of detection is lower than the minimal biological infestation unit (one miracidium). DNA extracts from Paramphistomum daubneyi, Dicrocoelium lanceolatum, and Fascioloides magna did not cross react.

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The potential impact of exotic Lymnaea spp. on fascioliasis in Australasia

Cited by 95 publications

References 15 publications

Fasciola hepatica: The influence of the definitive host on the characteristics of infection in the snailLymnaea truncatula

Fasciola hepatica: The influence of the definitive host on the characteristics of infection in the snailLymnaea truncatula

Several observations concerning cercarial sheddings ofFasciola giganticafromLymnaea natalensis

An optimized DNA extraction and multiplex PCR for the detection of Fasciola sp. in lymnaeid snails

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