The potential for bio-optical imaging of biomaterial-associated infection in vivo

Sjollema, Jelmer; Sharma, Prashant K.; Dijkstra, René J. B.; Dam, Gooitzen M. van; Mei, Henny C. van der; Engelsman, Anton F.; Busscher, Henk J.

doi:10.1016/j.biomaterials.2009.11.068

Cited by 53 publications

(47 citation statements)

References 70 publications

(118 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genetically engineered bioluminescent and fluorescent bacteria are promising for monitoring infection in animal studies but are limited exclusively to research, and the signal intensity depends upon many factors including cell count, metabolic rate, absorption and scattering in the tissue, and collection/emission optics. [5] In addition, optical scattering severely limits the image resolution. [6] …”

Section: Introductionmentioning

confidence: 99%

X‐Ray Excited Luminescence Chemical Imaging of Bacterial Growth on Surfaces Implanted in Tissue

Wang

Raval

Tzeng

et al. 2015

Adv Healthcare Materials

View full text Add to dashboard Cite

We develop a pH sensor film that can be coated on an implant surface and imaged using a combination of X-ray excitation and visible spectroscopy to monitor bacterial infection and treatment of implanted medical devices (IMDs) through tissue. X-ray scintillators in the pH sensor film generate light when an X-ray beam irradiates them. This light first passes through a layer containing pH indicator that alters the spectrum according to pH, then passes through tissue where it is detected by a spectrometer. A reference region on the film is used to account for spectral distortion from wavelength-dependent absorption and scattering in the tissue. pH images are acquired by moving the sample relative to the X-ray beam and collecting a spectrum at each location, with a spatial resolution limited by the X-ray beam width. Using this X-ray excited luminescence chemical imaging (XELCI) to map pH through porcine tissue, we detected a pH drop during normal bacterial growth on the sensor surface, and a restoration of the pH to the bulk value during antibiotic treatment over the course of hours with millimeter resolution. Overall, XELCI provides a novel approach to noninvasively image surface pH for studying implant infections and treatments.

show abstract

Section: Introductionmentioning

confidence: 99%

X‐Ray Excited Luminescence Chemical Imaging of Bacterial Growth on Surfaces Implanted in Tissue

Wang

Raval

Tzeng

et al. 2015

Adv Healthcare Materials

View full text Add to dashboard Cite

show abstract

“…These multimodality imaging technologies are becoming increasingly accessible as commercially-available imaging systems have been developed to observe and quantify the spatial and temporal distribution of optical signals from a living animal in 3D [6], [26]. In addition, this multimodality optical and anatomical imaging may be particularly effective in studying biological processes that involve dynamic changes in bone [26]–[28]. Therefore, in this study, we attempted to use multimodality optical and anatomical imaging to better evaluate noninvasively and longitudinally the bacterial burden and neutrophilic inflammation in the context of the pathologic changes that occur in bone in our mouse model of orthopaedic implant infection.…”

Section: Introductionmentioning

confidence: 99%

Monitoring Bacterial Burden, Inflammation and Bone Damage Longitudinally Using Optical and μCT Imaging in an Orthopaedic Implant Infection in Mice

et al. 2012

View full text Add to dashboard Cite

BackgroundRecent advances in non-invasive optical, radiographic and μCT imaging provide an opportunity to monitor biological processes longitudinally in an anatomical context. One particularly relevant application for combining these modalities is to study orthopaedic implant infections. These infections are characterized by the formation of persistent bacterial biofilms on the implanted materials, causing inflammation, periprosthetic osteolysis, osteomyelitis, and bone damage, resulting in implant loosening and failure.Methodology/Principal FindingsAn orthopaedic implant infection model was used in which a titanium Kirshner-wire was surgically placed in femurs of LysEGFP mice, which possess EGFP-fluorescent neutrophils, and a bioluminescent S. aureus strain (Xen29; 1×103 CFUs) was inoculated in the knee joint before closure. In vivo bioluminescent, fluorescent, X-ray and μCT imaging were performed on various postoperative days. The bacterial bioluminescent signals of the S. aureus-infected mice peaked on day 19, before decreasing to a basal level of light, which remained measurable for the entire 48 day experiment. Neutrophil EGFP-fluorescent signals of the S. aureus-infected mice were statistically greater than uninfected mice on days 2 and 5, but afterwards the signals for both groups approached background levels of detection. To visualize the three-dimensional location of the bacterial infection and neutrophil infiltration, a diffuse optical tomography reconstruction algorithm was used to co-register the bioluminescent and fluorescent signals with μCT images. To quantify the anatomical bone changes on the μCT images, the outer bone volume of the distal femurs were measured using a semi-automated contour based segmentation process. The outer bone volume increased through day 48, indicating that bone damage continued during the implant infection.Conclusions/SignificanceBioluminescent and fluorescent optical imaging was combined with X-ray and μCT imaging to provide noninvasive and longitudinal measurements of the dynamic changes in bacterial burden, neutrophil recruitment and bone damage in a mouse orthopaedic implant infection model.

show abstract

“…The advent of in vivo imaging systems has significantly improved the analysis of biomaterial-associated infections 87 . Genetic engineering of bioluminescence genes into clinically relevant bacterial strains allows for in vivo monitoring of infection.…”

Section: Animal Models To Assess Infection and Bone Repairmentioning

confidence: 99%

Scaffold-based Anti-infection Strategies in Bone Repair

2014

View full text Add to dashboard Cite

Bone fractures and non-union defects often require surgical intervention where biomaterials are used to correct the defect, and approximately 10% of these procedures are compromised by bacterial infection. Currently, treatment options are limited to sustained, high doses of antibiotics and surgical debridement of affected tissue, leaving a significant, unmet need for the development of therapies to combat device-associated biofilm and infections. Engineering implants to prevent infection is a desirable material characteristic. Tissue engineered scaffolds for bone repair provide a means to both regenerate bone and serve as a base for adding antimicrobial agents. Incorporating anti-infection properties into regenerative medicine therapies could improve clinical outcomes and reduce the morbidity and mortality associated with biomaterial implant-associated infections. This review focuses on current animal models and technologies available to assess bone repair in the context of infection, antimicrobial agents to fight infection, the current state of antimicrobial scaffolds, and future directions in the field.

show abstract

The potential for bio-optical imaging of biomaterial-associated infection in vivo

Cited by 53 publications

References 70 publications

X‐Ray Excited Luminescence Chemical Imaging of Bacterial Growth on Surfaces Implanted in Tissue

X‐Ray Excited Luminescence Chemical Imaging of Bacterial Growth on Surfaces Implanted in Tissue

Monitoring Bacterial Burden, Inflammation and Bone Damage Longitudinally Using Optical and μCT Imaging in an Orthopaedic Implant Infection in Mice

Scaffold-based Anti-infection Strategies in Bone Repair

Contact Info

Product

Resources

About