The Potential Effect of Botulinum Toxin Type A on Human Dermal Fibroblasts: An In Vitro Study

Oh, Sang‐Ha; Lee, Young; Seo, Young‐Joon; Lee, Jeung‐Hoon; Yang, Jung Dug; Chung, Ho Yun; Cho, Byung C.

doi:10.1111/j.1524-4725.2012.02504.x

Cited by 56 publications

(48 citation statements)

References 14 publications

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“…In the study conducted by Xiao et al [22] on hypertrophic scars, a decrease in fibroblast count in the groups applied with BoNT-A was reported. In the study conducted by Oh et al [23] on human dermal fibroblasts, BoNT-A was determined to cause a decrease in fibroblast count and collagen amount. Decreasing fibroblast count in the high-dose BoNT-A group detected in our study was determined to be in correspondence with existing literature data.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of botulinum toxin type A effectiveness in preventing postoperative intraperitoneal adhesions

Dokur

Uysal

2017

Ann Surg Treat Res

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PurposePostoperative intraperitoneal adhesions (PIAs) are one of the most important problems surgeons have to face after laparotomies. In this study, we aimed to evaluate the effectiveness of local application of botulinum toxin type A (BoNT-A) in various dosages on the prevention of intra-abdominal adhesions in rats with experimental intra-abdominal adhesions.MethodsForty Wistar Albino female rats were randomly separated into 4 groups. The 4 groups were determined as follows: Control (group 1, n = 10); Sham (group 2, n = 10); 10-µg/kg low-dose BoNT-A (group 3, n = 10) and 30-µg/kg high-dose BoNT-A (group 4, n = 10). Subserosal injuries were created on the caecum of all rats. Laparotomy was performed on the fifth day. Adhesion scores, histopathological examination, and E-cadherin expression levels were evaluated.ResultsGeneral adhesion scores for groups 1 and 2 were determined to be significantly high when compared to group 4 (P < 0.001). A significant difference was also determined between groups 3 and 4 in terms of general adhesion scores (P < 0.05). In pair comparisons, a significant decrease in high-dose BoNT-A group (group 4) when compared to groups 1 and 2 in terms of neovascularization, fibroblast density, collagen deposition and inflammatory cell count was determined (P < 0.05).ConclusionA significant decrease was observed only in postoperative PIAs in the high-dose BoNT-A group between all 4 rat-groups with experimentally created postoperative PIAs. In this study, high-dose BoNT-A is determined to be an effective agent in preventing postoperative PIAs.

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Section: Discussionmentioning

confidence: 99%

Evaluation of botulinum toxin type A effectiveness in preventing postoperative intraperitoneal adhesions

Dokur

Uysal

2017

Ann Surg Treat Res

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“…In addition, BoNT/A reduces the expression of cytokines and growth factors known to participate in scar forming (Xiao et al, 2010;Xiao et al, 2011;Xiaoxue et al, 2013). In cultured human fibroblasts, BoNT/A upregulated the collagen type I forming and decreased the expression of matrix metalloproteinases (Oh et al, 2012). Since fibroblasts do not express SNAP-25, observed BoNT/A effects might be mediated by other target molecules.…”

Section: Effects On Cell Cycle and Apoptosismentioning

confidence: 96%

Botulinum neurotoxin type A: Actions beyond SNAP-25?

Matak

Lacković

2015

Toxicology

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“…According to one study, BoNT/A may interrupt the differentiation of fibroblasts to myofibroblasts by blocking TGF‐β1 signalling . However, in another study, BoNT/A upregulated the expression of type I collagen and decreased the production of some MMPs . In Wistar rats, BoNT/A injections reduced wound and graft contraction, and in another rat model of burn wound healing, BoNT/A improved both the healing process and the cosmetic appearance of the burn scar, and was associated with faster regeneration, less inflammation and an increase in the number of fibroblasts…”

Section: Experimental Studies Of Cutaneous Effects Of Botulinum Neuromentioning

confidence: 99%

The non-neuronal and nonmuscular effects of botulinum toxin: an opportunity for a deadly molecule to treat disease in the skin and beyond

Grando

Zachary

2018

Br J Dermatol

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There is growing evidence that botulinum neurotoxins (BoNTs) exhibit biological effects on various human cell types with a host of associated clinical implications. This review aims to provide an update on the non-neuronal and nonmuscular effects of botulinum toxin. We critically analysed recent reports on the structure and function of cellular signalling systems subserving biological effects of BoNTs. The BoNT receptors and intracellular targets are not unique for neurotransmission. They have been found in both neuronal and non-neuronal cells, but there are differences in how BoNT binds to, and acts on, neuronal vs. non-neuronal cells. The non-neuronal cells that express one or more BoNT/A-binding proteins, and/or cleavage target synaptosomal-associated protein 25, include: epidermal keratinocytes; mesenchymal stem cells from subcutaneous adipose; nasal mucosal cells; urothelial cells; intestinal, prostate and alveolar epithelial cells; breast cell lines; neutrophils; and macrophages. Serotype BoNT/A can also elicit specific biological effects in dermal fibroblasts, sebocytes and vascular endothelial cells. Nontraditional applications of BoNT have been reported for the treatment of the following dermatological conditions: hyperhidrosis, Hailey-Hailey disease, Darier disease, inversed psoriasis, aquagenic palmoplantar keratoderma, pachyonychia congenita, multiple eccrine hydrocystomas, eccrine angiomatous hamartoma, eccrine sweat gland naevi, congenital eccrine naevus, Raynaud phenomenon and cutaneous leiomyomas. Experimental studies have demonstrated the ability of BoNT/A to protect skin flaps, facilitate wound healing, decrease thickness of hypertrophic scars, produce an anti-ageing effect, improve a mouse model of psoriasiform dermatitis, and have also revealed extracutaneous effects of BoNT arising from its anti-inflammatory and anticancer properties. BoNTs have a much wider range of applications than originally understood, and the individual cellular responses to the cholinergic impacts of BoNTs could provide fertile ground for future studies.

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The Potential Effect of Botulinum Toxin Type A on Human Dermal Fibroblasts: An In Vitro Study

Abstract: This study shows interesting effects of BoNT on collagen production and degradation of human dermal fibroblasts in vitro. This research provides the experimental background for using intradermal BoNT injection for remodeling of dermal tissues in aged skin.

Cited by 56 publications

References 14 publications

Evaluation of botulinum toxin type A effectiveness in preventing postoperative intraperitoneal adhesions

Evaluation of botulinum toxin type A effectiveness in preventing postoperative intraperitoneal adhesions

Botulinum neurotoxin type A: Actions beyond SNAP-25?

The non-neuronal and nonmuscular effects of botulinum toxin: an opportunity for a deadly molecule to treat disease in the skin and beyond

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