The Potency of some Natural Products on Dimethyl Benz ( A ) antheracene( DMBA ) Induced Hepatotoxicity in Rats

Abstract

Aim of the work:the present study was carried out to investigate the possible ameliorating effects of three herbs: hops(H) 1.5g/kg diet , rosemary(R) 5g/kg diet and cat's claw (C C) 1.7g/kg diet on hepatic toxicity induced by 7,12-Dimethyl benz(A)antheracene , a hydrocarbon involves various negative consequence for human health and ecosystems conversation .Results:in this work,48 female rats at 50 day of age were divided into 8 groups; control ,hops(H), rosemary(R), cat ' s claw(CC),DMBA, DMBA+H,DMBA+R and DMBA+CC groups.Results:the results indicated that a single intraperitonial (i.p) dose of DMBA (30mg/Kg b.W) caused significant decrease in the percentage of body weight gain, but an increase in the hepatosomatic index. In addition , the results illustrated an increase in the liver malondialdehyde (MDA) contents and hydrogen peroxide levels (H2O2) accompanied by significant decrease in reduced glutathione (GSH) content and superoxide dismutase (SOD) and glutathione-S-transferase (GST) activities.The results, also reported significant decrease in serum total proteins, total albumin , globulin and liver total protein but serum total bilirubin was significantly elevated in the DMBA intoxicated group. Furthermore, aspartate aminotransaminase (ASAT), alanine aminotransaminase (ALAT) , γ-glutamyltransferase (GGT) and alkaline phosphatase (ALP) activities were significantly increased in serum but significantly decreased in the liver. On the other hand, intake of hops, rosemary and cat ' s claw minimize the disturbances observed in most of the tested parameter's resulted from DMBA administration and improve the liver functions mostly in the following order, rosemary ˃ hops ˃ cat's claw. Conclusion: it can be concluded that intake of such herbs (hops , rosemary, cat ' s claw)may be effective in reducing DMBA toxicity.

Protective and Curative Effects of the Sea CucumberHolothuria atraExtract against DMBA-Induced Hepatorenal Diseases in Rats

“…It is well known that GSH is involved in the protection of normal cell structure and function in maintaining of the redox homeostasis and quenching of free radicals and by participating in detoxification reactions [ 55 ]. The present study confirmed the finding of Parmar et al [ 53 ] and El Kholy et al [ 45 ] who suggested that the enhancement of lipid peroxidation is a consequence of depletion of reduced glutathione (GSH) to certain critical levels. The depletion of GSH promotes generation of reactive oxygen species and oxidative stress with a cascade of effects, thereby affecting functional as well as the structural integrity of cell and organelle membranes [ 56 , 57 ].…”

“…The present study revealed that DMBA increased malondialdehyde concentration (MDA) in the liver tissue. These results are similar to the data reported by El Kholy et al [ 45 ] and Ahmed et al [ 46 ] who indicated that DMBA intake produced oxidative stress in liver of rats. The increased MDA level suggests enhanced lipid peroxidation leading to tissue damage and failure of antioxidant defense mechanisms to prevent formation of excessive free radicals.…”

“…The major cause of metabolic dysfunction during pathogenesis is oxidative damage of some of the susceptible amino acids of proteins [ 49 ]. In accord with the studies of Sharma et al [ 50 ] and El Kholy et al [ 45 ], the present study showed that DMBA intoxication decreased the serum total protein content. It was reported that decline in total protein can be deemed as a useful index of the severity of cellular dysfunction in chronic liver diseases as manifested by the severe histopathological alterations of the liver tissue following DMBA treatment.…”

“…The enhancement in CAT and SOD activities may be to prevent the accumulation of excessive free radicals and protect liver from DMBA intoxication. In agreement with the report of El Kholy et al [ 45 ] and Kumar et al [ 5 ], the liver cells have innate ability to arouse and maintain defense against oxidant by secreting more antioxidants. HaE may overpower DMBA onslaught by suppressing the formation of ROS and protecting the antioxidant machinery.…”

“…Superoxide dismutase (SOD) and catalase (CAT) act as mutually supportive antioxidative enzymes, which provide protective defense against reactive oxygen species [ 63 ]. Viewed in conjunction with the report of Parmar et al [ 54 ], El Kholy et al [ 45 ], and Kumar et al [ 5 ], the inhibition of CAT and SOD activities following DMBA intoxication in the present study may be due to the enhancement of the peroxidation end product MDA, which is known to inhibit protein synthesis and the activities of certain enzymes. Pre- or postadministration of HaE at the tested dose enhanced the activities of CAT and SOD in DMBA-induced liver damage.…”