The Potency of Endophytic Fungal Isolates Collected from Local Aromatic Rice as Indole Acetic Acid (IAA) Producer

Syamsia, Syamsia; Kuswinanti, Tutik; Syam’un, Elkawakib; Masniawati, Andi

doi:10.1016/j.profoo.2015.01.009

Cited by 33 publications

(19 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The bacteria did not show the ability to produce IAA. Although IAA-production ability is widespread among rhizobacteria but its production depends on several factors e.g., the potential of isolates to utilize tryptophan, sampling location, pH, oxygen availability, carbon conditions 39 , and the incubation time 40 . Longer incubation time causes decrease in nutrients found in growth media and possibly the amount of IAA produced consumed again by bacterial cells for their own growth.…”

Section: Discussionmentioning

confidence: 99%

First report of diazotrophic Brevundimonas spp. as growth enhancer and root colonizer of potato

Naqqash

Imran

Hameed

et al. 2020

Sci Rep

View full text Add to dashboard Cite

Rhizobacteria contain various plant-beneficial traits and their inoculation can sustainably increase crop yield and productivity. The present study describes the growth-promoting potential of Brevundimonas spp. isolated from rhizospheric soil of potato from Sahiwal, Pakistan. Four different putative strains TN37, TN39, TN40, and TN44 were isolated by enrichment on nitrogen-free malate medium and identified as Brevundimonas spp. based on their morphology, 16S rRNA gene sequence, and phylogenetic analyses. All strains contained nif H gene except TN39 and exhibited nitrogen fixation potential through acetylene reduction assay (ARA) except TN40. Among all, the Brevundimonas sp. TN37 showed maximum ARA and phosphate solubilization potential but none of them exhibited the ability to produce indole acetic acid. Root colonization studies using transmission electron microscopy and confocal laser scanning microscopy showed that Brevundimonas sp. TN37 was resident over the root surface of potato; forming sheets in the grooves in the rhizoplane. TN37, being the best among all was further evaluated in pot experiment using potato cultivar Kuroda in sterilized sand. Results showed that Brevundimonas sp. TN37 increased growth parameters and nitrogen uptake as compared to non-inoculated controls. Based on the results obtained in this study, it can be suggested that Brevundimonas spp. (especially TN37) possess the potential to improve potato growth and stimulate nitrogen uptake. This study is the first report of Brevundimonas spp. as an effective PGPR in potato.

show abstract

Section: Discussionmentioning

confidence: 99%

First report of diazotrophic Brevundimonas spp. as growth enhancer and root colonizer of potato

Naqqash

Imran

Hameed

et al. 2020

Sci Rep

View full text Add to dashboard Cite

show abstract

“…and Tulasnella sp.) could produce IAA after being cultured in liquid medium supplemented with L-Trp [ 7 , 10 – 13 , 16 , 17 , 19 , 49 ]. However, the present study provides the first report of IAA produced by C .…”

Section: Discussionmentioning

confidence: 99%

Biosynthetic pathway and optimal conditions for the production of indole-3-acetic acid by an endophytic fungus, Colletotrichum fructicola CMU-A109

et al. 2018

View full text Add to dashboard Cite

Endophytic fungi are known to produce indole-3-acetic acid (IAA), which can stimulate plant growth. Twenty-seven isolates of endophytic fungi were isolated from Coffea arabica in northern Thailand. Only one isolate (CMU-A109) produced IAA in vitro. This isolate was identified as Colletotrichum fructicola based on morphological characteristics and molecular phylogenetic analysis of a combined five loci (internal transcribed spacer of ribosomal DNA, actin, β-tubulin 2, chitin synthase and glyceraldehyde-3-phosphate dehydrogenase genes). Identification of a fungal IAA production obtained from indole 3-acetamide (IAM) and tryptophan 2-monooxygenase activity is suggestive of IAM routed IAA biosynthesis. The highest IAA yield (1205.58±151.89 μg/mL) was obtained after 26 days of cultivation in liquid medium supplemented with 8 mg/mL L-tryptophan at 30°C. Moreover, the crude fungal IAA could stimulate coleoptile elongation of maize, rice and rye. This is the first report of IAA production by C. fructicola and its ability to produce IAA was highest when compared with previous reports on IAA produced by fungi.

show abstract

“…The selected isolate PF5 was inoculated into 10 ml of Czapek dox medium (30 g sucrose, 2 g sodium nitrate, 1 g di‐potassium phosphate, 0.5 g magnesium sulfate 0.5 g potassium chloride, 0.01 g ferrous sulfate, and 1000 ml distilled water) supplemented with 0.2% (v/v) of L‐tryptophan and incubated for 10 days at 28°C. After incubation, the culture was centrifuged at 3000 rpm for 20 min and the supernatant was screened for presence of Indole acetic acid (Syamsia et al ). For this, 1 ml of culture supernatant was mixed with 2 ml of Salkowski reagent (2% of 0.5M FeCl 3 in 35% HClO 4 solution) and the tubes were incubated in the dark for 30 min.…”

Section: Methodsmentioning

confidence: 99%

“…Inoculation of PF5 to rice plant was done as in the previously described method (Syamsia et al ). Here rice seeds were soaked in 1% NaOCl for 1 min to remove surface contaminants.…”

Section: Methodsmentioning

confidence: 99%

Endophytic Phomopsis sp. colonization in Oryza sativa was found to result in plant growth promotion and piperine production

Chithra

Jasim

Mathew

et al. 2017

Physiologia Plantarum

View full text Add to dashboard Cite

Endophytic fungi have been reported to have the acquired ability to synthesize host plant specific medicinal natural products. Many fungi with such properties have been characterized and optimized for the conditions which favor maximal production of desired products. However, the inherent plant colonization property of promising endophytic fungi is least studied. Exploiting the transgenome functioning of these fungi have immense applications to add beneficial features to nonhost plants. In the present study, the endophytic fungus Phomopsis sp. isolated from Piper nigrum was confirmed for piperine production by HPLC and LCMS/MS. Further, the fungal isolate was studied for its colonization ability in Oryza sativa. Interestingly, the fungi treated plants were found to have significant plant growth enhancement when compared to the control. Further screening of extract from treated plants by HPLC and LCMS/MS resulted in the confirmation of presence of piperine. The observed result is extremely significant as it opens up novel applications of endophytic fungal colonization in taxonomically diverse plants.

show abstract

The Potency of Endophytic Fungal Isolates Collected from Local Aromatic Rice as Indole Acetic Acid (IAA) Producer

Cited by 33 publications

References 13 publications

First report of diazotrophic Brevundimonas spp. as growth enhancer and root colonizer of potato

First report of diazotrophic Brevundimonas spp. as growth enhancer and root colonizer of potato

Biosynthetic pathway and optimal conditions for the production of indole-3-acetic acid by an endophytic fungus, Colletotrichum fructicola CMU-A109

Endophytic Phomopsis sp. colonization in Oryza sativa was found to result in plant growth promotion and piperine production

Contact Info

Product

Resources

About