The potato protease inhibitor gene, St-Inh, plays roles in the cold-induced sweetening of potato tubers by modulating invertase activity

Liu, Xun; Cheng, Shanhan; Liu, Jun; Ou, Yongbin; Song, Botao; Zhang, Chi; Lin, Yang; Li, Xiuqing; Xie, Conghua

doi:10.1016/j.postharvbio.2013.07.001

Cited by 34 publications

(23 citation statements)

References 27 publications

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“…Our results reveal that StvacINV1 activity is directly inhibited by StInvInh2B (Table I), which confirms our previous finding that these two proteins specifically interact in potato tubers (Liu et al, 2013b). Our findings are also in accordance with other reports that overexpression of an invertase inhibitor in potato tubers reduces acid invertase activity (McKenzie et al, 2013), suggesting posttranslational regulation of invertase activity (Liu et al, 2013a). In fact, invertase inhibitors coexist with invertases widely in plant cells (Liu et al, 2010; therefore, it is worth investigating how invertase activity can be released in the competition with its inhibitor for developmental and stress responses.…”

Section: Stvacinv1 Activity Is Subtly Regulated By Stinvinh2b and Sbssupporting

confidence: 93%

“…However, this negative relationship between invertase activity and StInvInh2 expression seems to be regulated by environmental factors, as we found no detectable variations of invertase activity in the potato tubers stored at 20°C (Liu et al, 2013a(Liu et al, , 2013b. Other reports also propose that the inhibitor expression may be regulated by both environmental and developmental signals and to be genotype dependent (Johnson and Ryan, 1990;Turrà et al, 2009).…”

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confidence: 50%

“…Overexpression of a tobacco PMEI-RP gene, NtInvInh2, strongly reduced RS accumulation in potato tubers (Greiner et al, 1999). Similarly but much more weakly, the expression of a potato KPI gene, StInh, showed less effect on acid invertase activity and RS accumulation than NtInvInh2 (Liu et al, 2013a), implying that the PMEI-RPs may be more efficient than KPIs for repressing the acid invertase. There are two potato PMEI-RP invertase inhibitor genes, StInvInh2A and StInvInh2B, identified so far (Liu et al, 2010).…”

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confidence: 95%

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Subtle Regulation of Potato Acid Invertase Activity by a Protein Complex of Invertase, Invertase Inhibitor, and SUCROSE NONFERMENTING1-RELATED PROTEIN KINASE

Lin

Liu

et al. 2015

Plant Physiol.

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Slowing down cold-induced sweetening (CIS) of potato (Solanum tuberosum) tubers is of economic importance for the potato industry to ensure high-quality products. The conversion of sucrose to reducing sugars by the acid invertase StvacINV1 is thought to be critical for CIS. Identification of the specific StvacINV1 inhibitor StInvInh2B and the a-and b-subunits of the interacting protein SUCROSE NONFERMENTING1-RELATED PROTEIN KINASE from the wild potato species Solanum berthaultii (SbSnRK1) has led to speculation that invertase activity may be regulated via a posttranslational mechanism that remains to be elucidated. Using bimolecular fluorescence complementation assays, this study confirmed the protein complex by pairwise interactions. In vitro kinase assays and protein phosphorylation analysis revealed that phosphorylation of SbSnRK1a is causal for StvacINV1 activity and that its active form blocks the inhibition of StInvInh2B by SbSnRK1b, whereas its inactive form restores the function of SbSnRK1b that prevents StInvInh2B from repressing StvacINV1. Overexpression of SbSnRK1a in CIS-sensitive potato confirmed that SbSnRK1a has significant effects on acid invertase-associated sucrose degradation. A higher level of SbSnRK1a expression was accompanied by elevated SbSnRK1a phosphorylation, reduced acid invertase activity, a higher sucrose-hexose ratio, and improved chip color. Our results lend new insights into a subtle regulatory mode of invertase activity and provide a novel approach for potato CIS improvement.

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Section: Stvacinv1 Activity Is Subtly Regulated By Stinvinh2b and Sbssupporting

confidence: 93%

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confidence: 50%

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confidence: 95%

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Subtle Regulation of Potato Acid Invertase Activity by a Protein Complex of Invertase, Invertase Inhibitor, and SUCROSE NONFERMENTING1-RELATED PROTEIN KINASE

Lin

Liu

et al. 2015

Plant Physiol.

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“…In recent years, substantial molecular evidences have revealed carbohydrate metabolism involved in potato CIS. The most in-depth study is sucrose pathway in which the vacuolar acid invertase StvacINV1 and its specific inhibitor StInvInh2B play crucial roles in sucrose conversion to RSs ( Liu et al, 2010 , 2011 , 2013a , b ). Subsequent experiments have suggested that the inhibition of StvacINV1 by StInvInh2B is blocked by SbSnRK1β and restored by phosphorylated form of SbSnRK1α ( Lin et al, 2013 , 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Genetic Loci Conferring Reducing Sugar Accumulation and Conversion of Cold-Stored Potato Tubers Revealed by QTL Analysis in a Diploid Population

et al. 2018

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Cold-induced sweetening (CIS) caused by reducing sugar (RS) accumulation during storage in low temperature in potato tubers is a critical factor influencing the quality of fried potato products. The reconditioning (REC) by arising storage temperature is a common measure to lower down RS. However, both CIS and REC are genotype-dependent and the genetic basis remains uncertain. In the present study, with a diploid potato population with broad genetic background, four reproducible QTL of CIS and two of REC were resolved on chromosomes 5, 6, and 7 of the CIS-sensitive parent and chromosomes 5 and 11 of the CIS-resistant parent, respectively, implying that these two traits may be genetically independent. This hypothesis was also supported by the colocalization of two functional genes, a starch synthesis gene AGPS2 mapped in QTL CIS_E_07-1 and a starch hydrolysis gene GWD colocated with QTL REC_B_05-1. The cumulative effects of identified QTL were proved to contribute largely and stably to CIS and REC and confirmed with a natural population composed of a range of cultivars and breeding lines. The present research identified reproducible QTL for CIS and REC of potato in diverse conditions and elucidated for the first time their cumulative genetic effects, which provides theoretical bases and applicable means for tuber quality improvement.

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“…The search of new allelic variants of gene Pain-1 determining commercially significant attributes of potato and the assessment of the occurrence frequency of such variants is deemed important for optimization of the selection process [21][22][23].…”

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confidence: 99%

ALLELE DIVERSITY FOR ACID VACUOLAR INVERTASE GENE Pain-1 FRAGMENT IN POTATO (Solanum tuberosum L.) VARIETIES AND LINES

Слугина¹,

Shmelkova²,

Meleshin³

et al. 2018

S-h. biol.

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A b s t r a c tThe economic efficiency of potato production depends not only on the yield quantity and quality, but also on the tuber storage conditions. The tuber nutritional and technical value is determined by the starch content. Under the cold stress, the starch degrades into reducing sugars (coldsweetening), which significantly reduces the tuber quality. Plant invertases catalyze irreversible hydrolysis of sucrose into glucose and fructose. Nowadays it is definitely known that vacuolar acid invertase (Pain-1) plays a major role in the cold induced sweetening of potato tubers. In the present work genetic diversity and allelic polymorphism of Pain-1 gene associated with important agronomical traits of potato tubers is characterized. Gene fragment (exon 5-exon 7) polymorphism was analyzed in 69 cultivars and lines of Russian and foreign breeding origin. In the Pain-1 nucleotide sequences, 66 SNPs were identified, were described for the first time. The studied fragment contains a significant replacement for SNP1544 (C/A) which correlates with an increased starch content in the tubers and is homozygous in the Kazakhstani varieties Ulan and Astana. In the exons, 27 out of 42 SNPs led to amino acid substitutions. Most accessions had single amino acid substitutions. The maximum substitution number (seven to eight) characterized the Zhukovskii ranii variety and the lines 165 and 162. No substitutions were observed in the Frittella variety and the line 84. Therefore, the common level of gene fragment polymorphism in the analyzed potato accessions was shown to be rather high. Among the analyzed sequences, 78 allelic variants were described, including 64 specific variants and 14 variants common for several accessions. The obtained data may be helpful in potato breeding for an increase in starch content.

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The potato protease inhibitor gene, St-Inh, plays roles in the cold-induced sweetening of potato tubers by modulating invertase activity

Cited by 34 publications

References 27 publications

Subtle Regulation of Potato Acid Invertase Activity by a Protein Complex of Invertase, Invertase Inhibitor, and SUCROSE NONFERMENTING1-RELATED PROTEIN KINASE

Subtle Regulation of Potato Acid Invertase Activity by a Protein Complex of Invertase, Invertase Inhibitor, and SUCROSE NONFERMENTING1-RELATED PROTEIN KINASE

Genetic Loci Conferring Reducing Sugar Accumulation and Conversion of Cold-Stored Potato Tubers Revealed by QTL Analysis in a Diploid Population

ALLELE DIVERSITY FOR ACID VACUOLAR INVERTASE GENE Pain-1 FRAGMENT IN POTATO (Solanum tuberosum L.) VARIETIES AND LINES

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