The posterior neural plate in axolotl gives rise to neural tube or turns anteriorly to form somites of the tail and posterior trunk

Taniguchi, Yoshiaki; Kurth, Thomas; Weiche, Susanne; Reichelt, Saskia; Kato, Masaya; Perike, Srikanth; Kappert, Verena; Epperlein, Hans‐Henning

doi:10.1016/j.ydbio.2016.12.023

Cited by 19 publications

(38 citation statements)

References 54 publications

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“…in 32]. A number of studies suggest that neuromesodermal precursors (NMPs) are present in the vertebrate embryo posterior that will give rise to somite or spinal cord tissues [31,[33][34][35][36]. The co-expression of Bra/Sox proteins may be a transition state in these NMPs on their way to forming spinal cord.…”

Section: Bmp Can Activate Spinal Cord Markers Independently Of Mesodementioning

confidence: 99%

New roles for Wnt and BMP signaling in neural anteroposterior patterning

et al. 2019

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During amphibian development, neural patterning occurs via a two‐step process. Spemann's organizer secretes BMP antagonists that induce anterior neural tissue. A subsequent caudalizing step re‐specifies anterior fated cells to posterior fates such as hindbrain and spinal cord. The neural patterning paradigm suggests that a canonical Wnt‐signaling gradient acts along the anteroposterior axis to pattern the nervous system. Wnt activity is highest in the posterior, inducing spinal cord, at intermediate levels in the trunk, inducing hindbrain, and is lowest in anterior fated forebrain, while BMP‐antagonist levels are constant along the axis. Our results in Xenopus laevis challenge this paradigm. We find that inhibition of canonical Wnt signaling or its downstream transcription factors eliminates hindbrain, but not spinal cord fates, an observation not compatible with a simple high‐to‐low Wnt gradient specifying all fates along the neural anteroposterior axis. Additionally, we find that BMP activity promotes posterior spinal cord cell fate formation in an FGF‐dependent manner, while inhibiting hindbrain fates. These results suggest a need to re‐evaluate the paradigms of neural anteroposterior pattern formation during vertebrate development.

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Section: Bmp Can Activate Spinal Cord Markers Independently Of Mesodementioning

confidence: 99%

New roles for Wnt and BMP signaling in neural anteroposterior patterning

et al. 2019

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“…The lack of cell type-specific markers makes it difficult to study NMPs. Yet, the discovery of cells expressing both T/Brachyury (here referred to as T) and Sox2 in the region where NMPs are located has led many researchers to conclude that these are the NMPs 6,[9][10][11] . Indeed, co-expression of master regulators of mesoderm (T) and neural (Sox2) development fits well with the proposed potency of the dual-fated progenitors.…”

Section: Introductionmentioning

confidence: 99%

“…Indeed, co-expression of master regulators of mesoderm (T) and neural (Sox2) development fits well with the proposed potency of the dual-fated progenitors. Based on this assumption, T/Sox2 double-positive cells have been studied extensively in vitro [12][13][14][15] and in vivo 6,9,11,[16][17][18][19][20] . Despite the increasing interest in NMPs, their precise role in embryonic development still remains to be elucidated, though it is hypothesized that they are essential for body axis elongation 2,9,12,16,[20][21][22] .…”

Section: Introductionmentioning

confidence: 99%

Genetic approaches in mice demonstrate that neuro-mesodermal progenitors express T/Brachyury but not Sox2

Mugele

Moulding

Savery

et al. 2018

Preprint

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Neural tube and somites have long been thought to derive from separate germ layers: the ectoderm and mesoderm. This concept was challenged by the discovery of neuromesodermal progenitors, a bi-potent cell population that gives rise to both spinal neural tube and somites. In line with their proposed potency, these cells are considered to co-express the neural marker Sox2 and the mesodermal marker T/Brachyury. We performed genetic lineage tracing in mouse embryos and confirmed that T-expressing cells give rise to both neural tube and mesoderm. Surprisingly, however, Sox2-expressing cell derivatives colonise only the neural tube after embryonic day 8.5. Deletion of Sox2 in T-expressing cells was compatible with an otherwise normal neural tube and paraxial mesoderm. Moreover, Sox2 expression is absent from the chordoneural hinge, where neuro-mesodermal progenitors are located. Our findings demonstrate that neuro-mesodermal progenitors express T but notSox2, suggesting the need for re-evaluation of the neuro-mesodermal progenitor hypothesis.The initial aim of this study was to define the role of NMPs in neural tube formation, since it is not clear to what extent the progenitors contribute to this tissue. We traced cells either from the NSB or CLE into the closing neural tube, and found that the caudal end of the embryo harbours two distinct populations, both of which fulfil the criteria of NMPs, yet give rise to different domains within the closed neural tube. NMP function was addressed using laser ablation and a genetic approach, based on the proposed co-expression of T and Sox2.

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“…Since then, many different studies have used the grafting approach to provide a further detailed understanding of evolution, development, and tissue regeneration in axolotl. 30,[44][45][46][47][48]…”

Section: Ubiquitous Fluorescent Reportersmentioning

confidence: 99%

The use of transgenics in the laboratory axolotl

Tilley

Papadopoulos

Pende

et al. 2021

Developmental Dynamics

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The ability to generate transgenic animals sparked a wave of research committed to implementing such technology in a wide variety of model organisms.Building a solid base of ubiquitous and tissue-specific reporter lines has set the stage for later interrogations of individual cells or genetic elements. Compared to other widely used model organisms such as mice, zebrafish and fruit flies, there are only a few transgenic lines available in the laboratory axolotl (Ambystoma mexicanum), although their number is steadily expanding. In this review, we discuss a brief history of the transgenic methodologies in axolotl and their advantages and disadvantages. Next, we discuss available transgenic lines and insights we have been able to glean from them. Finally, we list challenges when developing transgenic axolotl, and where further work is needed in order to improve their standing as both a developmental and regenerative model.

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The posterior neural plate in axolotl gives rise to neural tube or turns anteriorly to form somites of the tail and posterior trunk

Cited by 19 publications

References 54 publications

New roles for Wnt and BMP signaling in neural anteroposterior patterning

New roles for Wnt and BMP signaling in neural anteroposterior patterning

Genetic approaches in mice demonstrate that neuro-mesodermal progenitors express T/Brachyury but not Sox2

The use of transgenics in the laboratory axolotl

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