The post-analytical phase of histopathology practice: Storage, retention and use of human tissue specimens

Kapila, Supriya Nikita; Boaz, Karen; Natarajan, Srikant

doi:10.4103/2229-516x.173982

Cited by 12 publications

(5 citation statements)

References 17 publications

(61 reference statements)

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“…In our experience, up to ten-year-old FFEP blocks have been used. Provided that storage is accurately done and the pre-analytical parameters indicated in this chapter are met, blocks can be preserved up to this time [17]. Since FFPE tissue is currently used for genetic analysis, results should always be carefully interpreted.…”

Section: Tissue Fixationmentioning

confidence: 99%

Molecular Pathology in the New Age of Personalized Medicine

Denninghoff¹

2021

Pathology - From Classics to Innovations

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Personalized medicine is a new approach that allows the identification of patients that can benefit from targeted therapies because of the molecular characteristics of the tumors they present. The molecular profile of the tumor can be studied at the genomic (DNA), transcriptomic (RNA) or protein (protein) level. The next generation sequencing is a useful tool for the study of molecular profile from DNA/RNA. This tool requires molecular pathologists highly trained in pre-analytic processes, tumor area microdissection for tumor cell enrichment, methodology analysis and results. The in-depth study of molecular alterations in patients allows optimizing molecular diagnosis and selecting candidates for receive novel treatments against specific molecular targets. These patients are expected to benefit from multidisciplinary approach and learning. The aim of this chapter is to show the implications of molecular pathology in personalized medicine with an actual approach from the methodological limitations of formalin-fixed paraffin embedded (FFPE) tissues and their pre-analytical conditions.

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Section: Tissue Fixationmentioning

confidence: 99%

Molecular Pathology in the New Age of Personalized Medicine

Denninghoff¹

2021

Pathology - From Classics to Innovations

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“…[139][140][141] Pathologists have taken on the key role of stewards of human tissue specimens for diagnosis and research into the future. 165 Applications of health informatics technology are making increasingly prevalent telecommunications between physicians and scientists and between professionals and patients and subject participants. 166 Telepathology is being facilitated by advances in telecommunications coupled with advances in digital and quantitative morphology.…”

Section: Impact Of Scientific Advances On Pathologymentioning

confidence: 99%

“…166 Telepathology is being facilitated by advances in telecommunications coupled with advances in digital and quantitative morphology. [164][165][166][167] Digital microscopy also is being coupled with artificial intelligence and deep computer learning. 168,169 The latter is better termed augmented intelligence, representing a powerful adjunct for the human operator.…”

Section: Impact Of Scientific Advances On Pathologymentioning

confidence: 99%

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The Texas Society of Pathologists: molded by the legacy of pathology and focused on excellence in medicine for 100 years and beyond

Buja

2020

Baylor University Medical Center Proceedings

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In 1921, 16 Texas pathologists gathered in Dallas, Texas, to found the Texas Society of Pathologists (TSP). The TSP is now the oldest state pathology society in the USA with continuity traced back to its founding 100 years ago. This article aims to both commemorate the TSP centennial and to provide context for the remarkable success of the society. The article takes a look back and a look forward from 1921. The look back focuses on the development of the field of pathology and the maturation of medicine and pathology in the USA and Texas. The look forward encompasses developments in science, technology, American health care policy, and medicine that have impacted Texas pathologists and influenced proactive initiatives of the TSP. The review of the life and times of the TSP highlights the importance of leaders and leadership in shaping outcomes. Complexities and uncertainties of the contemporary health care scene point to the need for continued strong leadership. The successful past century and hopeful future of the TSP are inextricably linked to the guiding principle of the TSP, which is a focus on continual striving for excellence in medicine. KEYWORDS Health care policy; history; Texas society of pathologists "I have great respect for the past. If you don't know where you've come from, you don't know where you are going."-Maya Angelou (1928-2014) "Of necessity an historical account must be largely biographical. Men and their books have built pathology. Yet without a point of view which takes account of the major social movements of general history, no real conception of the historical development of any subject is possible."-Esmond R. Long, MD (1890-1979) "If I have seen further than others, it is by standing upon the shoulders of giants."-Isaac Newton (1642-1726)

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“…Finally, in a clinical scenario, a complete audit-trail of any diagnostic analyses must be retained for quality and risk management purposes. Currently, medical accreditation organizations require every laboratory in the United States to store biopsy slides for a minimum of 10 years 7 ; similarly, future medicolegal concerns could require long-term storage of millions of sub-images (or the means to reproduce them).…”

Section: Introductionmentioning

confidence: 99%

PathFlowAI: A High-Throughput Workflow for Preprocessing, Deep Learning and Interpretation in Digital Pathology

et al. 2019

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The diagnosis of disease often requires analysis of a biopsy. Many diagnoses depend not only on the presence of certain features but on their location within the tissue. Recently, a number of deep learning diagnostic aids have been developed to classify digitized biopsy slides. Clinical workflows often involve processing of more than 500 slides per day. But, clinical use of deep learning diagnostic aids would require a preprocessing workflow that is cost-effective, flexible, scalable, rapid, interpretable, and transparent. Here, we present such a workflow, optimized using Dask and mixed precision training via APEX, capable of handling any patch-level or slide level classification and prediction problem. The workflow uses a flexible and fast preprocessing and deep learning analytics pipeline, incorporates model interpretation and has a highly storage-efficient audit trail. We demonstrate the utility of this package on the analysis of a prototypical anatomic pathology specimen, liver biopsies for evaluation of hepatitis from a prospective cohort. The preliminary data indicate that PathFlowAI may become a cost-effective and time-efficient tool for clinical use of Artificial Intelligence (AI) algorithms.

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The post-analytical phase of histopathology practice: Storage, retention and use of human tissue specimens

Cited by 12 publications

References 17 publications

Molecular Pathology in the New Age of Personalized Medicine

Molecular Pathology in the New Age of Personalized Medicine

The Texas Society of Pathologists: molded by the legacy of pathology and focused on excellence in medicine for 100 years and beyond

PathFlowAI: A High-Throughput Workflow for Preprocessing, Deep Learning and Interpretation in Digital Pathology

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