The possible pre -and post -UVA radiation protective effect of amaranth oil on human skin fibroblast cells

Abstract

Background:The health effects of Amaranth Oil (AO) are attributed to its specific chemical composition. That makes it an outstanding natural product for the prevention and treatment of ultraviolet (UV) irradiation-related pathologies such as sunburn, photoaging, photoimmunosuppression, and photocarcinogenesis. Most of the studies are taken on animal model, and there is a lack of research on the endogenous effect of AO on fibroblast level, where UVA takes it harmful place.Objective:The aim of this study was evaluation if AO can protect or abolish UVA exposure effect on human skin fibroblast.Materials and Methods:The 0.1% AO, 0.25% AO, and 0.5% AO concentration and irradiation for 15 min under UVA-emitting lamp were studied in various condition. In all experiments, the mean values for six assays ± standard deviations were calculated.Results:Pretreatment with various concentrations of AO was tested. The highest concentration of AO where cell survival was observed was 0.5%. Cytotoxicity assays provided evidence for pre- and post-UVA protective effect of 0.1% AO among three tested concentrations. The results also provide evidence that UVA has inhibitory effect on collagen biosynthesis in confluent skin fibroblast, but presence of 0.1% AO abolishes pre- and post-UVA effect comparing to other used AO concentration. The assessment results on DNA biosynthesis show the significant abolished post-UVA effect when 0.1% and 0.5% of AO were added.Conclusion:AO gives pre- and post-UVA protection in low concentration. This provides the evidence for using it not as a main protective factor against UV but as one of the combined components in cosmetic formulation.SUMMARY The recommended Amaranth Oil (AO) concentration in cosmetic formulation is between 0.1 and 5%Pretreatment with various concentrations of AO suggests to use the highest 0.5% concentration of AO in human skin fibroblast culturesThe 0.1% of AO in fibroblast cultures, protects and abolishes effect of ultraviolet A (UVA) exposureUVA has inhibitory effect on collagen biosynthesis in skin fibroblast, but presence of 0.1% AO abolishes pre- and post-UVA effectThe abolished post-UVA effect occurs when 0.1% and 0.5% of AO were added on DNA biosynthesis. Abbreviations used: AO: Amaranth Oil.

Protective Effect of Amaranthus cruentus L. Seed Oil on UVA-Radiation-Induced Apoptosis in Human Skin Fibroblasts

“…Due to its unique chemical composition, AmO exhibits cytoprotective and antioxidant potential, suggesting its promising application as a UV-radiation-protective substance for skin care purposes [ 15 ]. We found that the effect of AmO on cell viability varied depending on the concentration used.…”

“…Our studies indicated a cytotoxic effect of UVA radiation on human skin fibroblasts and its involvement in the generation of significant amounts of ROS. Considering that ROS are suppressors of basic cellular functions, as well as pro-apoptotic factors [17], we investigated the protective-antioxidant potential of AmO at selected concentrations in Due to its unique chemical composition, AmO exhibits cytoprotective and antioxidant potential, suggesting its promising application as a UV-radiation-protective substance for skin care purposes [15]. We found that the effect of AmO on cell viability varied depending on the concentration used.…”

Protective effect of curcumin against ultraviolet A irradiation‑induced photoaging in human dermal fibroblasts

“…Furthermore, an increasing amount of evidence indicates that the majority of basal cell carcinomas may be primarily attributed to UVA irradiation ( 4 , 5 ). To protect human dermal fibroblasts (HDFs) against the harmful effects of UVA, natural compounds are often employed, including Coenzyme Q10, vitamin C, vitamin E and Amaranth Oil ( 6 – 8 ).…”

Ultraviolet A protective potential of plant extracts and phytochemicals

“…A recent study evaluated effect of pre-and post-treatment with amaranth oil on UVAirradiated human skin fibroblasts. The amaranth oil activity was rather poor and its use in combination with other sunscreens is recommended 82 .…”