The possible macrophage role in seminal fluid

Solís, Eloy; Bouvet, Beatriz Reina; Brufman, A.S.; Feldman, Robert G.; Gatti, Vanda Nora

doi:10.4321/s0210-48062003000300003

Cited by 5 publications

(6 citation statements)

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“…This immunoglobulin is de ned as an antisperm antibody. The results from this study show that there were fewer AsAb-positive samples in the lower concentration group of macrophages (2.9% vs 9.1%), but there was no signi cant statistical difference (P > 0.05), which is different from that of related studies [7]. This was mainly due to the fact that we excluded the high-risk population of AsAb in the initial screening of the sample.…”

Section: Discussioncontrasting

confidence: 89%

“…There are a small number of macrophages in semen which play important roles in maintaining sperm quality [7]. They originate from the interstitial tissue of the testes and epididymis, accounting for 25% of the total testicular stromal cells [10].…”

Section: Discussionmentioning

confidence: 99%

“…Next we used the software to make a dot plot of all the collected cells through CD14-FITC, and we used the FITC positive area as gate P1. According to the Solis [7], the samples were divided into a low concentration group (Mφ<6×10 5 /ml) and a high concentration group (Mφ>6×10 5 /ml).…”

Section: Analysis Of Macrophagesmentioning

confidence: 99%

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Effect of Macrophages in Semen on Sperm Quality

Chen

Zheng

2020

Preprint

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Background: This was a cross-sectional study in China, we analyzed the levels of macrophages(Mφ) in semen. The study evaluated the influence of the levels of Mφ in semen on sperm quality.Methods: The subjects were 78 males between 25 and 35 years old. The samples were divided into a low group(Mφ<6×105/ml) and a high group (Mφ>6×105/ml). Evaluation included consideration of the influencing factors of male semen quality, macrophage concentration, sperm motility, morphology, membrane integrity DNA fragmentation index (DFI), anti-sperm antibodies (AsAb), IL-10, and IL-12 in semen.Results: There was no difference in the physical or chemical indices of the semen, sperm concentration, AsAb, IL-10, or IL-12 between the two groups (P>0.05). The percentage of sperm forward motility (PR%), the rate of normal sperm shape, and the integrity of cell membranes in the low group were higher than those in the high group (P<0.05), while the percentage of sperm inactivity (IM%), the rate of sperm head deformity, the rate of deformity in the neck and middle segment, the sperm malformation index (SDI), the abnormal sperm index (TZI), and the sperm DFI in the low group were lower than those in the high group (P<0.05). The concentration of Mφ in the semen was linearly correlated with sperm concentration, sperm PR%, IM%, sperm normal shape rate, head deformity rate, neck and middle deformity rate, SDI, TZI, sperm DFI, and sperm cell membrane integrity (P<0.05), but there was no linear correlation with IL-10 or IL-12 (P>0.05).Conclusions: The concentration of Mφ in semen had no significant correlation with semen volume or sperm concentration, but it did have a significant negative correlation with sperm motility, sperm morphology, cell membrane integrity, and DNA breakage rate. There was no significant correlation with the concentration of IL-10 or IL-12.

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Section: Discussioncontrasting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

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Effect of Macrophages in Semen on Sperm Quality

Chen

Zheng

2020

Preprint

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“…Other authors have observed a direct relationship between the presence of macrophages and the number of sperm exhibiting denatured DNA. 25 High-load endurance training promotes the appearance of muscle injury markers along with an increased white cell count in blood. 26 During inflammation, additional circulating macrophages help testes-residing macrophages by arriving and invading the site of inflammation.…”

Section: Discussionmentioning

confidence: 99%

Findings on sperm alterations and DNA fragmentation, nutritional, hormonal and antioxidant status in an elite triathlete. Case report

Vaamonde

Grigoletto

Fernández

et al. 2014

Revista Andaluza de Medicina del Deporte

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a b s t r a c tObjective: The present case study analyzes semen quality, nutritional patterns, and hormonal and oxidative status of an international high-level triathlete with a low-volume, high-intensity training load. Method: The athlete was 26 years old, having participated in competitions since he was 13 years old, and practiced professional triathlon for the last five years. The qualitative sperm parameters analyzed were volume, sperm count, motility, morphology, and DNA fragmentation (additional testing performed as needed). Salivary hormones measured were T, C, and T/C. Seminal plasma total antioxidant capacity was measured. Maximum oxygen consumption and training characteristics were assessed. To determine habitual food intake and its possible repercussion on oxidative status, a quali-quantitative frequency questionnaire of 136 different foods was used and subsequently analyzed using specific software. Results: Hormonal and physical semen parameters were within normal ranges. Sperm morphology and DNA fragmentation showed abnormal values (3.9% and 38.33%). Round cells in semen were higher than normal (2.3 million/ml), with presence of macrophages. Apoptotic and necrotic events were observed. Total antioxidant capacity, although not compromised, was low. Dietetic intake was characterized by excess protein and appropriate overall antioxidant intake (with slight deficit and excess of some specific nutrients) according to recommended values. Conclusions: In this high-intensity endurance athlete, sperm parameters, mainly sperm morphology and DNA fragmentation, are altered. Further knowledge is needed with regards nutritional antioxidant intake and other dietetic strategies oriented toward avoiding oxidative damage in semen of high-performance triathletes. Moreover, adequate nutritional strategies must be found and nutritional advice given to athletes so as to palliate or dampen the effects of exercise on semen quality. Hallazgo de alteraciones en semen, nutricionales, hormonales, del estado antioxidante y fragmentación de ADN en un triatleta de élite. Caso clínicoPalabras clave: Entrenamiento de resistencia Triatlón Semen Hormonas Estado oxidativo Nutrición Fragmentación ADN r e s u m e n Objetivo: El presente estudio de caso analiza la calidad de semen, patrones nutricionales y status hormonal y oxidativo de un triatleta internacional de alto nivel con carga de entrenamiento de bajo volumen y alta intensidad. Método: Atleta de 26 años de edad, con una práctica deportiva competitiva desde los 13 años, y cinco años de triatlón profesional. Los parámetros cualitativos analizados en semen fueron: volumen, contaje espermático, motilidad, morfología, y fragmentación de ADN, con pruebas adicionales según necesidad. Las hormonas salivares cuantificadas fueron: T, C, y T/C. La capacidad antioxidante total fue medida en plasma seminal. También se analizó el consumo máximo de oxígeno y características de entrenamiento. Para determinar la ingesta alimentaria habitual y su posible repercusión sobre el estado oxidativo, se ...

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“…Activated macrophages are capable of harming cells such as sperm through three principal mechanisms (Anderson, 1990; Solis et al. , 2003).…”

Section: Discussionmentioning

confidence: 99%

Macrophage activity in semen is significantly correlated with sperm quality in infertile men

Tremellen

Tunc

2010

Int J of Andrology

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The presence of leucocytes within semen has the potential to impair sperm function. Neutrophils and macrophages make up 95% of seminal leucocytes, with both having the ability to damage sperm via the generation of reactive oxygen species, proteases and the induction of apoptosis. Existing cytological techniques for quantifying leucocyte activity within semen (peroxidase, CD45) are less than ideal as they merely count the number of leucocytes, rather than assess their activity. Seminal plasma elastase effectively determines neutrophil activity, yet gives no insight into macrophage activity. Neopterin, a molecule released from activated macrophages, may be a useful marker for macrophage activity in the male reproductive tract. To examine this possibility a total of 63 asymptomatic subjects with male factor infertility and 11 fertile controls provided semen samples for measurement of various inflammatory markers. We were able to confirm for the first time that seminal plasma does indeed contain neopterin and that the levels of this macrophage activity marker are threefold higher in infertile than fertile men. Furthermore, seminal plasma neopterin concentration was significantly correlated with sperm oxidative stress, DNA fragmentation (TUNEL) and apoptosis (Annexin V), making it a useful marker of sperm quality. By contrast, seminal plasma elastase showed no correlation with any marker of sperm quality.

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The possible macrophage role in seminal fluid

Cited by 5 publications

References 1 publication

Effect of Macrophages in Semen on Sperm Quality

Effect of Macrophages in Semen on Sperm Quality

Findings on sperm alterations and DNA fragmentation, nutritional, hormonal and antioxidant status in an elite triathlete. Case report

Macrophage activity in semen is significantly correlated with sperm quality in infertile men

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