The possible macrophage role in seminal fluid

Solís, Eloy; Bouvet, Beatriz Reina; Brufman, A.S.; Feldman, Rodolfo Néstor; Gatti, Vanda Nora

doi:10.1016/s0210-4806(03)72903-9

Cited by 11 publications

(10 citation statements)

References 5 publications

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“…A close relationship is present between the neopterin released from macrophages and their capacity for producing ROS ( 3 ). Activated macrophages have two ways to damage the sperm: first, by producing high levels of reactive oxygen and nitrogen species that will lead to sperm membrane lipid peroxidation and oxidative DNA damage; secondly by releasing lysosomal enzymes and cytotoxic peptides ( 33 , 34 ). Neopterin has been observed in various biological samples such as serum, cerebrospinal fluid, synovial fluid, ascitic fluid, urine, saliva, and pancreatic secretions ( 5 , 35 ).…”

Section: Discussionmentioning

confidence: 99%

Association between seminal plasma neopterin and oxidative stress in male infertility: A case-control study

Ghiasvand

Goodarzi

Shafiee

et al. 2018

IJRM

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Background: Neopterin is a significant and sensitive marker in estimating the activity of cellular immune system. Oxidative stress plays a role in the etiology of male infertility. Increased reactive oxygen species is accompanied with increase in neopterin level. Hence neopterin may be involved in male infertility. Objective: The objective of this case-control study was to determine neopterin level in idiopathic infertile and normospermic men; furthermore, to identify its relationship with oxidative stress markers including total oxidant, malondialdehyde, sperm DNA fragmentation, and total antioxidant capacity of seminal plasma. Materials and Methods: Forty seven infertile and forty three normospermic males were selected according to WHO criteria. Their semen and blood samples were taken; subsequently, the levels of neopterin, total oxidant, total antioxidant, malondialdehyde, and sperm DNA fragmentation were measured. Results: The levels of neopterin, total oxidant, and malondialdehyde in seminal plasma of infertile males were significantly higher than those of normospermic group (p=0.038, 0.018, and 0.028, respectively). Furthermore, sperm DNA fragmentation in infertile men was higher than that of control group (p<0.001). Moreover, total antioxidant capacity of seminal plasma in infertile males was significantly lower than that of normospermic subjects (p=0.002). No significant difference was observed in serum neopterin, total oxidant, and malondialdehyde between the infertile and normospermic groups. Conclusion: The significant inverse correlation between seminal plasma neopterin and total antioxidant in the infertile males supports a possible role of neopterin in male infertility. Neopterin can be suggested as a marker in monitoring and diagnosis of idiopathic male infertility.

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Section: Discussionmentioning

confidence: 99%

Association between seminal plasma neopterin and oxidative stress in male infertility: A case-control study

Ghiasvand

Goodarzi

Shafiee

et al. 2018

IJRM

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“…Activated macrophages are capable of harming cells such as sperm through three principal mechanisms (Anderson, 1990;Solis et al, 2003). Firstly, they are capable of producing large amounts of reactive oxygen and nitrogen species that will lead to sperm membrane LPO and oxidative DNA damage.…”

Section: Discussionmentioning

confidence: 99%

Macrophage activity in semen is significantly correlated with sperm quality in infertile men

Tremellen

Tunc

2010

Int J of Andrology

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The presence of leucocytes within semen has the potential to impair sperm function. Neutrophils and macrophages make up 95% of seminal leucocytes, with both having the ability to damage sperm via the generation of reactive oxygen species, proteases and the induction of apoptosis. Existing cytological techniques for quantifying leucocyte activity within semen (peroxidase, CD45) are less than ideal as they merely count the number of leucocytes, rather than assess their activity. Seminal plasma elastase effectively determines neutrophil activity, yet gives no insight into macrophage activity. Neopterin, a molecule released from activated macrophages, may be a useful marker for macrophage activity in the male reproductive tract. To examine this possibility a total of 63 asymptomatic subjects with male factor infertility and 11 fertile controls provided semen samples for measurement of various inflammatory markers. We were able to confirm for the first time that seminal plasma does indeed contain neopterin and that the levels of this macrophage activity marker are threefold higher in infertile than fertile men. Furthermore, seminal plasma neopterin concentration was significantly correlated with sperm oxidative stress, DNA fragmentation (TUNEL) and apoptosis (Annexin V), making it a useful marker of sperm quality. By contrast, seminal plasma elastase showed no correlation with any marker of sperm quality.

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“…Also, it is possible that maternal APC may be prone to death or dysfunctional in migration since the primary cells involved in this decidual reaction are phagocytes that are thought to ensure the clearance of microorganisms and seminal debris remaining in the tract after insemination (72). Direct presentation of male MHC Ags may also have been mediated by seminal or testicular macrophages or immature DC that entered the semen upon ejaculation (73)(74)(75), since the paternal APC in uterine flushes only activated effector CD8 ϩ T cells. However, neither direct nor cross-presenting APC stimulated responses of either naive or effector/memory CD8 ϩ T cells in vivo.…”

Section: Discussionmentioning

confidence: 99%

Paternal Antigen-Bearing Cells Transferred during Insemination Do Not Stimulate Anti-Paternal CD8+ T Cells: Role of Estradiol in Locally Inhibiting CD8+ T Cell Responses

Seavey

Mosmann

2006

The Journal of Immunology

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Maternal immunological tolerance of the semiallogeneic fetus involves several overlapping mechanisms to balance maternal immunity and fetal development. Anti-paternal CD8+ T cells are suppressed during pregnancy in some but not all mouse models. Since semen has been shown to mediate immune modulation, we tested whether exposure to paternal Ag during insemination activated or tolerized anti-paternal CD8+ T cells. The uterine lumen of mated female mice contained male MHC I+ cells that stimulated effector, but not naive, CD8+ T cells ex vivo. Maternal MHC class I+ myeloid cells fluxed into the uterine lumen in response to mating and cross-presented male H-Y Ag to effector, but not naive, CD8+ T cells ex vivo. However, neither unprimed nor previously primed TCR-transgenic CD8+ T cells specific for either paternal MHC I or H-Y Ag proliferated in vivo after mating. These T cells subsequently responded normally to i.p. challenge, implicating ignorance rather than anergy as the main reason for the lack of response. CD8+ T cells responded to either peptide Ag or male cells delivered intravaginally in ovariectomized mice, but this response was inhibited by systemic estradiol (inducing an estrus-like state). Subcutaneous Ag induced responses in both cases. Allogeneic dendritic cells did not induce responses intravaginally even in ovariectomized mice in the absence of estradiol. These results suggest that inhibition of antiallogeneic responses is restricted both locally to the reproductive tract and temporally to the estrous phase of the menstrual cycle, potentially decreasing the risk of maternal immunization against paternal Ags during insemination.

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The possible macrophage role in seminal fluid

Cited by 11 publications

References 5 publications

Association between seminal plasma neopterin and oxidative stress in male infertility: A case-control study

Association between seminal plasma neopterin and oxidative stress in male infertility: A case-control study

Macrophage activity in semen is significantly correlated with sperm quality in infertile men

Paternal Antigen-Bearing Cells Transferred during Insemination Do Not Stimulate Anti-Paternal CD8+ T Cells: Role of Estradiol in Locally Inhibiting CD8+ T Cell Responses

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