The possible involvement of protein synthesis in the injection of PL-1 phage genome into its host, Lactobacillus casei

Watanabe, Kenji; Shirabe, Mitsuhiro; Nakashima, Yukihiko; Kakita, Yukari

doi:10.1099/00221287-137-11-2601

Cited by 15 publications

(9 citation statements)

References 16 publications

(10 reference statements)

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“…In this regard, the energy requirement to carry out some stages of the lytic cycle (DNA injection) was previously studied in lactic acid bacteria. Specifically, the presence of intracellular high-energy compounds was demonstrated to be indispensable for penetration of phage PL-1 genome into the bacterial host cell ( L. casei ) [ 14 , 15 , 21 , 25 ]. In addition, phage PL-1 adsorbed on starved cells but the DNA injection into the host cell was inhibited due to the absence of an active cell metabolism (decrease of intracellular ATP content, inhibition of protein synthesis).…”

Section: Resultsmentioning

confidence: 99%

Phage adsorption and lytic propagation in Lactobacillus plantarum: Could host cell starvation affect them?

2015

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BackgroundBacteriophages constitute a great threat to the activity of lactic acid bacteria used in industrial processes. Several factors can influence the infection cycle of bacteriophages. That is the case of the physiological state of host cells, which could produce inhibition or delay of the phage infection process. In the present work, the influence of Lactobacillus plantarum host cell starvation on phage B1 adsorption and propagation was investigated.ResultFirst, cell growth kinetics of L. plantarum ATCC 8014 were determined in MRS, limiting carbon (S-N), limiting nitrogen (S-C) and limiting carbon/nitrogen (S) broth. L. plantarum ATCC 8014 strain showed reduced growth rate under starvation conditions in comparison to the one obtained in MRS broth. Adsorption efficiencies of > 99 % were observed on the starved L. plantarum ATCC 8014 cells. Finally, the influence of cell starvation conditions in phage propagation was investigated through one-step growth curves. In this regard, production of phage progeny was studied when phage infection began before or after cell starvation. When bacterial cells were starved after phage infection, phage B1 was able to propagate in L. plantarum ATCC 8014 strain in a medium devoid of carbon source (S-N) but not when nitrogen (S-C broth) or nitrogen/carbon (S broth) sources were removed. However, addition of nitrogen and carbon/nitrogen compounds to starved infected cells caused the restoration of phage production. When bacterial cells were starved before phage infection, phage B1 propagated in either nitrogen or nitrogen/carbon starved cells only when the favorable conditions of culture (MRS) were used as a propagation medium. Regarding carbon starved cells, phage propagation in either MRS or S-N broth was evidenced.ConclusionsThese results demonstrated that phage B1 could propagate in host cells even in unfavorable culture conditions, becoming a hazardous source of phages that could disseminate to industrial environments.

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Section: Resultsmentioning

confidence: 99%

Phage adsorption and lytic propagation in Lactobacillus plantarum: Could host cell starvation affect them?

2015

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“…In phage ATCC 15807‐B1, it is possible that the process requiring Ca 2+ is the penetration of phage DNA inside the bacterial cell, as has been shown for the phages PL‐1 ( Lact. casei ) (Watanabe et al . 1991) and 0448 ( Lact.…”

Section: Discussionmentioning

confidence: 99%

“…With regard to phage receptors in lactobacilli, the most studied has been that of strain Lact. casei S1 for phages PL‐1 and J‐1 (Watanabe et al . 1991, 1993; Klaenhammer & Fitzgerald 1994).…”

Section: Discussionmentioning

confidence: 99%

Physicochemical characterization of phage adsorption toLactobacillus helveticusATCC 15807 cells

Quiberoni

Reinheimer

1998

Journal of Applied Microbiology

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A . Q UI B ER ON I AN D J . A. RE I NH EI M ER . 1998. Characterization of the adsorption process by the phages hv and ATCC 15807-B1 to Lactobacillus helveticus ATCC 15807 was carried out. For this purpose, the influence of Ca 2+ ions, temperature and physiological cell state were studied. The ability of several saccharides and related compounds to inactivate the phages hv and ATCC 15807-B1 was determined to investigate their potential role as phage receptors. Furthermore, several chemical treatments on the sensitive strain cells were carried out to study their influence on phage adsorption. Cell lysis and plaque formation were independent of Ca 2+ ions for phage hv, but the cation was indispensable for completion of the lytic cycle of phage ATCC 15807-B1. However, for this phage, Ca 2+ was not necessary for the adsorption process. The adsorption rates were almost normal for both phages within the temperature range examined (0 -50°C) and the adsorption kinetics were practically identical on viable and non-viable cells. The saccharides and related compounds used did not produce inactivation of the phages, suggesting that they were not essential components of phage receptor structures. Lactobacillus helveticus ATCC 15807 cells treated with SDS 1%, SDS 0·5% -EDTA 50 mmol l −1 or NaOH 50 mmol l −1 exhibited reduced adsorption of the phages, indicating possible damage or extraction of receptors from the cell wall. Phage adsorption presents an extremely attractive target for interfering in the lytic cycle of phages.

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“…For Lb. Watanabe et al (1991) have examined injection of Lb. While adsorption of phage PL-l occurred normally in the absence of Ca++, this cation was shown to be essential for phage DNA penetration as was A TP (Watanabe and Takesue, 1972;Watanabe et al, 1979).…”

Section: 2i Interaction With Hostsmentioning

confidence: 99%

Genetics and Biotechnology of Lactic Acid Bacteria

Gasson¹,

Vos²

1994

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The possible involvement of protein synthesis in the injection of PL-1 phage genome into its host, Lactobacillus casei

Cited by 15 publications

References 16 publications

Phage adsorption and lytic propagation in Lactobacillus plantarum: Could host cell starvation affect them?

Phage adsorption and lytic propagation in Lactobacillus plantarum: Could host cell starvation affect them?

Physicochemical characterization of phage adsorption toLactobacillus helveticusATCC 15807 cells

Genetics and Biotechnology of Lactic Acid Bacteria

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