The possible bottleneck effect of polyamines' catabolic enzymes in efficient adventitious rooting of two stone fruit rootstocks

Tsafouros, Athanasios; Roussos, Peter A.

doi:10.1016/j.jplph.2019.152999

Cited by 9 publications

(3 citation statements)

References 47 publications

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“…Due to the high number of processes in which polyamines seem to take part, it is difficult to clearly establish their role in AR. Besides, the activity of their catabolic enzymes and the concomitant generation of reactive oxygen species add another layer of complexity to their putative role in this developmental process [ 58 ].…”

Section: Biochemical Profiles During Armentioning

confidence: 99%

Recent Advances in Adventitious Root Formation in Chestnut

Vielba

Vidal

José

et al. 2020

Plants

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The genus Castanea includes several tree species that are relevant because of their geographical extension and their multipurpose character, that includes nut and timber production. However, commercial exploitation of the trees is hindered by several factors, particularly by their limited regeneration ability. Regardless of recent advances, there exists a serious limitation for the propagation of elite genotypes of chestnut due to decline of rooting ability as the tree ages. In the present review, we summarize the research developed in this genus during the last three decades concerning the formation of adventitious roots (ARs). Focusing on cuttings and in vitro microshoots, we gather the information available on several species, particularly C. sativa, C. dentata and the hybrid C.sativa × C. crenata, and analyze the influence of several factors on the achievements of the applied protocols, including genotype, auxin treatment, light regime and rooting media. We also pay attention to the acclimation phase, as well as compile the information available about biochemical and molecular related aspects. Furthermore, we considerate promising biotechnological approaches that might enable the improvement of the current protocols.

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Section: Biochemical Profiles During Armentioning

confidence: 99%

Recent Advances in Adventitious Root Formation in Chestnut

Vielba

Vidal

José

et al. 2020

Plants

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“…shoots in vitro [30] and was related to in vitro rooting of walnut [31], suggesting a consistent favorable effect for several species. Nevertheless, 5-hydroxy-1,4-naphthoquinone is a known allelopathetic factor that can induce the production of reactive oxygen species (ROS), such as H 2 O 2 [32], the application of which can increase rooting in Prunus cuttings [33], whereas its scavenging can inhibit rooting [34]. Thus, the effect of the quinones on ARF may rely on direct and/or indirect action, through the production of rooting promoting molecules, such as H 2 O 2 , or the regulation of hormonal levels, such as auxin.…”

Section: Discussionmentioning

confidence: 99%

Dopamine, Chlorogenic Acid, and Quinones as Possible Cofactors of Increasing Adventitious Rooting Potential of In Vitro Krymsk 5 Cherry Rootstock Explants

Tsafouros

Roussos

2022

Agronomy

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In the present study, the effect of some not commonly used phenolic compounds was evaluated during the in vitro rooting stage of the cherry rootstock ‘Krymsk 5′ (P. fruticosa × P. lannesiana), in the absence or presence of auxin. Two sets of experiments were conducted. In the first set, the following substances were tested: the o-diphenol chlorogenic acid, in five concentrations (0 μΜ, 0.5 μΜ, 1 μΜ, 5 μΜ, and 50 μΜ) in the presence of a suboptimal indolebutyric acid (IBA) concentration (5 μΜ), the catecholamine dopamine in five concentrations (0 μΜ, 0.5 μΜ, 1 μΜ, 5 μΜ, and 50 μΜ), and the quinone 5-hydroxy-1,4-naphthoquinone in four concentrations (0 μΜ, 0.25 μΜ, 1 μΜ, and 5 μΜ) in the absence or presence of 5 μΜ IBA. In the second experiment, the quinones p-benzoquinone; 1,4-napthoquinone; and 2-hydroxy-1,4-naphthoquinone were tested in four concentrations (0 μΜ, 5 μΜ, 50 μΜ, and 100 μΜ) in the presence of 5 μΜ IBA. An application of 5 μΜ of 5-hydroxy-1,4-naphthoquinone in the auxin-free medium increased rooting potential almost 1.7 times. Rooting percentage was also enhanced up to 4.2 times by dopamine; chlorogenic acid; 5-hydroxy-1,4-naphthoquinone; p-benzoquinone; and 1,4 napthoquinone in the presence of IBA. The present results indicate a possible promotive role of quinones and dopamine during in vitro rooting, at least for Prunus species, and their potential use as rooting cofactors. Moreover, a possible mode of action of the compounds studied related to IAA-oxidase is discussed.

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“…Indeed, lowering the apoplastic pH levels may cause cell wall loosening, expediting the morphogenic changes [54,55]. In accordance, treatments with H 2 O 2 that provoke cell wall creep have been used to induce responses, such as adventitious rooting [56].…”

Section: Effects Of Ph In "Krymsk 5 ® " In Vitro Propagationmentioning

confidence: 99%

In Vitro Propagation of Commercially Used Krymsk 5® (Prunus fruticosa × Prunus lannesiana) Cherry Rootstock: Impact of Sugar Types and pH Levels

Tsafouros,

Roussos

2024

Agriculture

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In the present study, the effects of different types of sugars and cultivation medium pH levels on the micropropagation of Krymsk 5® cherry rootstock were investigated. During the proliferation stage, the effects of four sugars (sucrose, fructose, glucose, and sorbitol) both separately and in two combinations were studied, along with the effects of pre-adjusted pH (4.5, 5.0, 5.2, 5.5, 5.8, 6.0, 6.2, or 6.5) on shoot proliferation parameters, growth medium’s post-autoclaving and post-cultivation pH, and their relations. Similarly, during the rooting stage, the effects of four sugars (sucrose, glucose, fructose, or sorbitol) at three concentrations (1% w/w, 2% w/w, or 3% w/w) without any auxin inclusion were studied as well as the effects of two sugars (sucrose or fructose) at six pre-adjusted pH levels (4.8, 5.2, 5.8, 6.2, or 6.5), also in the absence of auxin, on rooting parameters. Explants cultivated in fructose-supplemented growth mediums exhibited superior proliferation performance, characterized by the highest values of shoots per explant, shoot length, and nodes per explant. Generally, the medium’s pH decreased after autoclaving, and proliferation performance was favored by low pH values (either pre-adjusted or post-autoclaving). As far as rooting is concerned, fructose inclusion induced a higher rooting percentage (88%) compared to sucrose. The highest rooting was obtained in fructose-supplemented rooting mediums at concentrations of 2% or 3% w/w (95% rooting in both cases), in the absence of auxins. Post-autoclaving pH in fructose-supplemented rooting mediums was lower and buffered in low pH levels than in sucrose-supplemented ones, and the rooting of explants in all pH combinations with fructose exceeded 75%. In addition, rooting was negatively correlated with the post-autoclaving pH. These findings underscore the significance of both the sugar type and the post-autoclaving pH of the medium in both proliferation and rooting stages, highlighting their possible physiological, biochemical, or hormonal effects. Additionally, rooting without the use of auxin, but with the correct choice of sugar, emerges with both financial and environmental benefits, whereas fructose could be potentially used as a buffering agent.

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The possible bottleneck effect of polyamines' catabolic enzymes in efficient adventitious rooting of two stone fruit rootstocks

Cited by 9 publications

References 47 publications

Recent Advances in Adventitious Root Formation in Chestnut

Recent Advances in Adventitious Root Formation in Chestnut

Dopamine, Chlorogenic Acid, and Quinones as Possible Cofactors of Increasing Adventitious Rooting Potential of In Vitro Krymsk 5 Cherry Rootstock Explants

In Vitro Propagation of Commercially Used Krymsk 5® (Prunus fruticosa × Prunus lannesiana) Cherry Rootstock: Impact of Sugar Types and pH Levels

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