The possible ameliorative effect of vitamin D3 and/or omega-3 fatty acids in a rat model of type I early diabetic nephropathy: A physiological and histological study

Abstract

Diabetic nephropathy (DN) is one of the most common causes of chronic kidney diseases (CKDs) that have been associated with high morbidity and mortality.Recently, several studies have highlighted the protective role of vitamin D3 (VD3) and omega-3 fatty acids (O3-FAs) in CKDs. However, their effect on DN is still unclear.Aims: This study aimed to evaluate the effects of both VD3 and / or O3-FAs in slowing the progression of DN through their impact on indices of renal function, glycemic control, oxidative stress and markers of podocyte injury. Materials and methods: type I diabetes was induced in albino rats by single intraperitoneal injection of streptozotocin (65mg/kg). Rats received daily oral administration of VD3 and O3-FAs separately and in combination for 6 weeks. Results: VD3 and O3-FAs therapy improved significantly hyperglycemia, renal function tests, with concomitant decrease in total urinary protein content, urinary nephrin, a marker of podocyte injury and renal oxidative stress. However, the combined therapy was superior in its effect over VD3 and O3-FAs separately. Such results were confirmed by renal cortical tissue assessment using light microscopic examination of H&E and PAS stains in addition to transmission-electron microscopy. Conclusions: VD3 and O3-FAs have a potential beneficial effect on amelioration of structural changes in pedicels and glomerular basement membrane that was reflected as evident renal functional restoration.

Capability of Autologous Platelet Rich Plasma to Alleviate Experimentally Induced Diabetic Nephropathy in Adult Male Albino Rat (Light and Electron Microscopic Study)

“…Similar changes in the structure of the renal glomeruli with degenerative changes in the renal tubules were reported by Ebrahim et al (23) and El-Sokkary et al (24) in untreated diabetic rats.…”

“…Nephrin and Podocin proteins are integral proteins of the slit diaphragms which are essential for the construction of the filtration barrier. Defective production of these proteins occurs in diabetic nephropathy leading to abnormal thickening of glomerular basement membrane and alteration of the filtration process (25)(26) .…”