The Possibilities of the Conductivity Method as Applied to Studies of Bacterial Metabolism

Parsons, L. B.; Sturges, W. S.

doi:10.1128/jb.11.3.177-188.1926

Cited by 10 publications

(9 citation statements)

References 2 publications

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“…of ammonia formed and A C to the increase in specific conductivity of the medium. A similar proportionality was reported by Parsons and Sturges (1926a, b) in their work on conductivity and ammonia production by certain putrefactive anerobes. Their proportionality constant was, however, somewhat greater, which means that an increase in conductivity represented the synthesis of more ammonia in their media inoculated with putrefactive anerobes than in the skim milk medium inoculated with P. fluorescens.…”

Section: Experiments On Proteolysissupporting

confidence: 87%

“…on October 9, 2020 by guest http://jb.asm.org/ Downloaded from than that described for P. fluorescens, the proportionality constant is higher. Parsons and Sturges (1926a, b) also found that the increase in conductivity was proportional to the increase in the nitrogen calculated from a formol titration; in other words to the increase in ammonia plus the increase in amino nitrogen. The data obtained on P. fluorescens shown in figure 3 illustrate such a proportionality.…”

Section: LImentioning

confidence: 87%

“…Several difficult problems are therefore presented in attempting to evaluate conductivity measurements in relation to bacterial metabolism. Parsons and Sturges (1926a, b) have reported a definite correlation between conductivity and the amount of ammonia and amino nitrogen produced by putrefactive anaerobes. This is the only direct relationship between conductivity and some product of metabolism which has been established so far.…”

mentioning

confidence: 95%

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The Method of Electrical Conductivity in Studies on Bacterial Metabolism

1938

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Section: Experiments On Proteolysissupporting

confidence: 87%

Section: LImentioning

confidence: 87%

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confidence: 95%

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The Method of Electrical Conductivity in Studies on Bacterial Metabolism

1938

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“…It was first mentioned at a meeting of the British Medical Association at Edinburgh in July 1898 where Stewart presented a paper later to be published in The Journal of Experimenta 1 Medicine entitled 'The changes produced by the growth of bacteria in the molecular concentration and electrical conductivity of culture media' (Stewart 1899). The electrical response curves presented followed the putrefaction of blood and serum and were very similar to those obtained from currently available impedance systems, the significant difference being that today impedance can be considered as a rapid microbiological method whereas Stewart was measuring changes in impedance over periods in excess of 30 d. Further work followed on from Stewart's initial findings (Oker-Blom 1912 ;Parsons and Sturges 1926;Parsons et al 1929;Allison et al 1938;McPhillips and Snow 1958). However, it was not until the mid seventies that the technique began to receive the attention it merited.…”

Section: Introductionmentioning

confidence: 54%

Impedance microbiology—a rapid change for microbiologists

Silley

Forsythe

1996

Journal of Applied Bacteriology

179

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“…The bacteria culture is a heterogeneous and complex one that is constantly changing as seen from the pH variation. There is a direct correlation between conductivity measurements in relation to bacteria metabolism products; (Parsons and Sturges 1926). Sierakowski and Leczyeka (1983) have reported that there is always some relationship between electrical conductivity and metabolic processes even though the direct cause for the alteration in electrical field strength may be obscure.…”

Section: Discussionmentioning

confidence: 99%

Microbial Enhanced Oil Recovery

2022

Green Energy and Technology

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This Ph.D. dissertation dealt with microbial enhanced oil recovery focusing on adaptation of bacteria to some of subsurface conditions and the mechanisms of enhanced oil recovery through the use of microbes and their metabolic products.The core of this thesis consists of eight articles mostly dedicated to combination of different chemical and physical laboratory methods for experimentation, analysis and interpretation. These include flow injection, fermentation process, chromatographic assay, spectroscopy and image analysis to provide new, simple and robust solutions to understanding of processes during microbial enhanced oil recovery. A review of microbial enhanced oil recovery was presented to better comprehend the problem. The importance of mathematical models used in predicting the structural and morphological of bacteria cells during adaptation stages has been qualitatively discussed. The roles of biogenic acids and gases in carbonate rock dissolution and re-pressurization during microbial fluid rock interactions were also highlighted. The adapted bacteria strain were tested in different in formation waters from the North Sea and also, evaluated for improvement in oil recovery from packed columns by injection of bacteria solution to mimic in-situ oil recovery.Paper I and II presents models that explain the relationships between environmental parameters of pH, electrical conductivity, salinity and gas dissolution based on simple empirical models. This is valuable for understanding some of the interactions in the subsurface during the enhance oil recovery. The measured salinity is similar to those found in oil reservoirs.Paper III gives an overview of the adapted strain of Clostridium tyrobutyricum. The main objective of this study was to investigate the growth and metabolic products capability of this adapted strain and the potential to enhance oil recovery at elevated salinity. It was elaborated that quantities of some of the metabolites, gas, acids and biofilms have direct relationships with salinity of the medium and recovery of 38 % from sandstone and 25 % from chalk was achievable.Paper IV-V highlighted the microbial fluid rock interactions. It was found that porosity increase observed in all the rock samples was mainly due to significant dissolution of carbonate by the organic acids produced during microbial metabolism. The patterns of dissolution lead to reduction in the bulk volume of the chalk samples. The pore volumes were slightly reduced or generally remain the same and the release of Ca 2+ ions into microbial medium.First of all, I wish to give glory to the Almighty, who has given me good health, strength and wisdom in the past decades and the grace to complete this project. During the course of this study, a number of people have provided help and support that have added value in no small amount to the completion of this work, to which I am very grateful. First of all, I would like to thank the Aalborg University for providing me the opportunity to carry out this Ph.D. research. It has been a wo...

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The Possibilities of the Conductivity Method as Applied to Studies of Bacterial Metabolism

Cited by 10 publications

References 2 publications

The Method of Electrical Conductivity in Studies on Bacterial Metabolism

The Method of Electrical Conductivity in Studies on Bacterial Metabolism

Impedance microbiology—a rapid change for microbiologists

Microbial Enhanced Oil Recovery

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